• Research in Plant Disease
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• v.16 no.2
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• pp.129-135
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• 2010

PCR primers were developed to detect Pseudomonas savastanoi pv. phaseolicola, a causal agent of halo blight that occurs in all species of common bean (Phaseolus vulgaris L.), from the bean seeds. A primer set, Psp-JHF and Psp-JH-R, specifically amplified 513 bp fragment from Pseudomonas savastanoi pv. phaseolicola only. A nested primer set, psp-JH-F-ne and psp-JH-R-ne, designed from the $1^{st}$ PCR amplicon, amplified 169 bp fragment. The primer sets did not amplify any non-specific DNA from the seed extracts of Fabaceae including 4 beans, 2 soybeans, and 2 peas. The detection sensitivity of the nested PCR method developed in this study was much higher than that of ELISA and selective medium. PCR assays developed in this study should be useful to detect Pseudomonas savastanoi pv. phasolicola from the bean seeds.

• Journal of Science Education
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• v.41 no.1
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• pp.36-59
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• 2017

The purpose of this study was to develop a theory appropriate to the context by identifying the career motivation formation process from the perspective of academic high school students using a grounded theory approach. In this study, participants were selected among non probability sampling was used for sampling by artificially reputational case selection methods. Forty two highschool students(21 male, 21 female) were participated in this study. Research data were collected mainly collected using in-depth interview data were analyzed by applying the grounded theory method of Strauss and Corbin (1998). According to the results, 319 concepts and 56 sub-categories, and 19 categories were derived n the open coding process. Academic high school students' formation of STEM career motivation were influenced by contextual conditions of "STEM-related career think that the instruments' causal conditions and 'STEM education experience in inside and outside of school' named 'STEM career understanding and self-understanding' using the strategy of being influenced in interventional conditions of social support and obstacle 'for the central phenomenon of "STEM efforts to achieve career goals, it appeared as a result of" satisfaction for STEM careers. And it had a 5-step process over time that the formation process of STEM career motivation. This is expected to provide homes, schools, communities, and contribute to have a new insight on the education of the country, given the direction of career education and counseling intervention and the basic data used to develop and apply STEM career education.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.273-277
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• 2011

Fusarium oxysporum f. sp. fragariae (Fof), the causal agent of crown and root rot in strawberry, is the most serious soilborne disease of nursery plants in Korea. The possibility of infection by Fof through runner propagation from infected mother plants of strawberry cv. 'Kumhyang' was assessed in stolons and daughter plants hanging from raised beds. The number of daughter plants from an infected mother plant in plastic house and photosynthetic photon flux (PPF) system, 280 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ was 2.7 and 3.8 plants after 58 days, respectively. However, healthy mother plants produced 6.5 and 8.4 daughter plants, respectively. The pathogen was detected in the uppermost portion of the stolon after 58 days, but was not detected further down the stolon. After 90 days, it was detected in all portions of the stolon between mother and $1^{st}$ daughter plant and in 60% of all $1^{st}$ daughter plants. The pathogen was not detected in the corresponding portions of the non-infected controls. These results show that infected mother plants can transmit Fof to their daughter plants without passing through the soil and $1^{st}$ daughter was used as mother plant in PPF system for propagating healthy plants.

• Culinary science and hospitality research
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• v.19 no.3
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• pp.218-233
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• 2013

This study used SPSSS 18.0 on 237 university students in Busan for frequency, factorial and reliability, correlation, and regression analyses to determine the effect relationship of coffee shop service quality on perceived value and behavioral intention. Multiple regression analysis for hypothesis testing showed that among the four service quality factors, confidence in employees and primary coffee quality had a non-significant effect on esthetic value, while menu characteristics besides coffee (${\beta}$=.293, p<.001) and physical environment (${\beta}$=.293, p<.001) were analyzed to be significant, partially supporting the study hypothesis. Regarding the effect of service quality on practical value, confidence in employees (${\beta}$=.264 p<.001), primary coffee quality (${\beta}$=.463, p<.001), menu characteristics besides coffee (${\beta}$=.139, p<.05) and physical environment (${\beta}$=.110, p<.05) were all significantly analyzed, supporting the study hypothesis. Regarding the effect of service quality on behavioral intention, confidence in employees (${\beta}$=.262 p<.001), primary coffee quality (${\beta}$=.411, p<.001), menu characteristics besides coffee (${\beta}$=.157, p<.01) and physical environment (${\beta}$=.137, p<.05) were all significantly analyzed, supporting the study hypothesis. In addition, regarding the effect of perceived value on behavioral intention, esthetic value (${\beta}$=.265, p<.001) and practical value (${\beta}$=.536, p<.001) were both significantly analyzed, showing a causal relationship with behavioral intention.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.8 no.3
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• pp.1-16
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• 2013

The purpose of study is to promote the development of medium size enterprises(MSE) by analyzing the causal relationship between technology acquisition strategy and firms' performance. In this study, we use the number of intellectual property, sales growth rates, and return on sales as proxy variables of technological and financial performances. This study includes internal R&D investment, cooperative R&D with academy, foreign technologies transferred, and their interaction effects as the independent variables. The results of study are as follows. Frist, R&D Investment, Foreign Technologies Transferred and their interaction effects are positively influence on technological performance. Second, Cooperative R&D with academy and the interaction effects of R&D Investment and Cooperative R&D with academy are negative influence on technological performance. On the other hand, all of the main independent variables and the interaction effects have non-significant relationships with financial performances. These results imply that technological innovation efforts of MSEs do not effectively increase their financial performances. Furthermore, technological supports of government and academy on MSEs' innovation do not effectively increase their innovation outputs. Finally, this study discussed its theoretical and practical implications and the limitations of the study.

• The Korean Journal of Mycology
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• v.26 no.1 s.84
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• pp.31-38
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• 1998

Since 1990, destructive yellow rots on Ganoderma lucidum caused by a soilborne fungus have been occurred in major cultivation areas of Korea. Incidences of the disease were 61% in Chulwon and 94% in Kanghwa area where the mushroom has been cultivated for 10 years, whereas the disease has not been found yet in new cultivation areas such as Moonkyung and Hongsung. when severely infected, inner tissues of bed-logs showed severe yellow and fruiting bodies of the mushroom was not produced. Infected tissues of bed-logs were readily distinguished from those of healthy ones by a distinctive brown border line. When the disease progressed, mycelia of Ganoderma lucidum were totally destroyed, and abundant ascocarps of the pathogen were formed inside the tissues of bed-logs showing yellowish green. The fungus derived from a single ascospore strongly lysed mycelia of Ganoderma lucidum growing on bottle media, and non-volatile components secreted by the pathogen were also highly inhibitory to mycelial growth of the mushroom fungus. The pathogen was identified as Arthrographis cuboidea based on its distinctive cultural and morphological characters. The fungus produced arthroconidia and unbrached conidiophores. The width of fungal conidia was distinctively wide as compared with the length. Colonies of the fungi were pale yellow to yellowish green on agar media. As a causal pathogen of yellow rot of Ganoderma lucidum., this fungus has not been reported yet in Korea.

• Research in Plant Disease
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• v.21 no.2
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• pp.82-88
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• 2015

Acidovorax citrulli, the causal agent of bacterial fruit blotch, has caused an economically destructive damage in cucurbits cultivation fields worldwide. To consider more effective disease management, 33 A. citrulli isolates collected from various cucurbits in Korea were analysed by multi-locus phylogeny using five conserved loci(16S rRNA, adk, gltA, glyA, pilT). Two distinct groups (KCC1 and KCC2) in the population were identified on the base of group-specific genetic variation. Out of them, the predominant group was KCC2 and both groups included isolates from melon, cucumber and watermelon. Sixty-four percent of KCC1 isolates were recovered from non-watermelon hosts and seventy-two percent of KCC2 isolates from watermelon. This study presented that there was genetic differentiation among A. citrulli population in Korea. Also, these results will be applied as a very useful data in effective disease management.

• Journal of Internet of Things and Convergence
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• v.7 no.3
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• pp.81-90
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• 2021

In this study, the effect of user characteristics and technical characteristics of a blockchain-based financial platform on the intention to use of financial consumers was analyzed. Also, in this influence relationship, we analyzed what kind of causal relationship between relative advantage and perceived risk on intention to use. From June 1 to July 30, 2021, a non-face-to-face self-filling online survey was conducted with a sample of subjects who had experience using a financial platform grafted with blockchain technology, and the study was conducted in 187 copies. For statistical processing, frequency analysis, exploratory factor analysis, reliability analysis, correlation analysis, multiple regression analysis and 3-step mediated regression analysis were performed using SPSS 21.0 program. The significance level of the statistical value was set to less than 95%. The research results are as follows. First, it was found that innovativeness and usefulness affect the intention to use in the user characteristics. Second, in the technical characteristics, compatibility and reliability were found to affect the intention to use. Third, it was found that relative advantage and perceived risk play a partial mediating role in the relationship between user characteristics and intention to use. Fourth, it was found that relative advantage and perceived risk play a partial mediating role in the relationship between technical characteristics and intention to use. Fifth, it was found that there were differences in the ubiquity of user characteristics, compatibility of technical characteristics and intention to use according to the experience of using the certificate. The results of this study can contribute to the development of a financial platform based on the Internet of Things.

• Convergence Security Journal
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• v.18 no.1
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• pp.143-155
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• 2018

The purpose of this study is to extract the failure attributions to identify the failure factors of the security guards and to derive the risk factor factors through failure cases analysis. Failure attributions were prepared by using semi-structured questionnaires(17) and closed questionnaires(179) starting from the fully open questionnaires(8), and processed through SPSS 21.0 and AMOS 21.O statistical packages. As a result of summarizing the causal relationship between the failure attribution and the failure case (patrol failure pattern factor), In Park Jung-Hee's assassination, lack of experience(2), negative mind(1), lack of work ability(1), lack of experience(2), organizational non-system activeness(2), lack of awareness of mission(1) Failure(2), lack of consciousness(2), and 14 failure patterns were found. Aung-San National Cemetery explosion occurred in eight failure patterns including insecurity(1), negative mind(1), lack of work skills(2), lack of experience(2), individualism(1), There were eight failure patterns in the case of Mr. Yook Young-Su 's sniping, including insincerity(2), negative mind (1), lack of experience(2), lack of awareness of mission(2) and failure to share work(1).

• The Korean Society of Law and Medicine
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• v.22 no.2
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• pp.49-79
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• 2021

Due to defendant's wrongful act by implant surgery, plaintiff has been suffered serious damages to his face and teeth, and pain caused by establishing implanted teeth. Jeonju Appellate Court sentenced to pay future medical expenses and alimony to the plaintiff in compensation for breach of duty or torts. The ruling is designed to relieve the burden of proof because it is extremely difficult for non-experts to determine whether dentists violated their 'duty of care' or whether there was a causal relationship between damages to medial treatment. It was judged that if symptoms that contributed to the patient's significant outcome occurred during or after surgery, such symptoms could be presumed to have been caused by medical negligence if indirect facts were proven to be other than medical negligence. Originally, the shifting of burden of proof in Germany, has already been developed in medical malpractice case since 1940s. In order to guarantee the patients' right, §630h German Civil Code (BGB) - presumption of negligence in the realization of controllable risk- has been also legislated. BGH (Bundesgerichtshof) has been interested in ensuring that the principle of equality between patients and doctors. So, in this study, we wanted to refer to German precedent cases to analyzing Korean medical malpractice lawsuit. In particular, the decision could be significant in that it approaches closer to allows the shifting burden of proof in drastically growing dental malpractice cases. This is clearly confirmed in the judgment of the dentist's "fault" that "if indirect facts about the symptom or occurrence are proven to be cause other than medical negligence, such symptoms can be presumed to be due to medical negligence."