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http://dx.doi.org/10.5423/RPD.2010.16.2.129

Development of PCR Primers to Detect Pseudomonas savastanoi pv. phaseolicola from the Bean Seeds  

Cho, Jung-Hee (Department of Plant Medicine, Chungbuk National University)
Jeong, Min-Jung (Department of Plant Medicine, Chungbuk National University)
Song, Min-Ji (Department of Plant Medicine, Chungbuk National University)
Yim, Kyu-Ock (National Quarantine Service)
Lee, Hyok-In (National Quarantine Service)
Kim, Jung-Hee (National Quarantine Service)
Baeg, Ji-Hyun (National Quarantine Service)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Publication Information
Research in Plant Disease / v.16, no.2, 2010 , pp. 129-135 More about this Journal
Abstract
PCR primers were developed to detect Pseudomonas savastanoi pv. phaseolicola, a causal agent of halo blight that occurs in all species of common bean (Phaseolus vulgaris L.), from the bean seeds. A primer set, Psp-JHF and Psp-JH-R, specifically amplified 513 bp fragment from Pseudomonas savastanoi pv. phaseolicola only. A nested primer set, psp-JH-F-ne and psp-JH-R-ne, designed from the $1^{st}$ PCR amplicon, amplified 169 bp fragment. The primer sets did not amplify any non-specific DNA from the seed extracts of Fabaceae including 4 beans, 2 soybeans, and 2 peas. The detection sensitivity of the nested PCR method developed in this study was much higher than that of ELISA and selective medium. PCR assays developed in this study should be useful to detect Pseudomonas savastanoi pv. phasolicola from the bean seeds.
Keywords
Halo blight pathogen; PCR assays; Pseudomonas savastanoi pv. phaseolicola; Seed detection;
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Times Cited By KSCI : 5  (Citation Analysis)
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