• Journal of Applied Biological Chemistry
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• 제53권1호
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• pp.8-12
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• 2010

The antioxidant activity of hawthorn fruit (Crataegus pinnatifida Bunge var. typica Schneider) extracts was investigated by several in vitro antioxidants properties, including DPPH free radical scavenging activity, total phenolic and flavonoid contents, hydroxyl radical scavenging activity, reducing power activity, iron-chelating capacity and nitrite scavenging activity. Among the extracts in this study, the 70% EtOH extract showed higher antioxidant activity than the others. The $IC_{50}$ value of DPPH free radical scavenging activity was $99.26\;{\mu}g/mL$. Furthermore, the 70% EtOH extract also showed significantly high total phenolic and flavonoids contents and reducing power activity. However, the MeOH extract exhibited stronger effects on hydroxyl radical scavenging activity, iron-chelating capacity and nitrite scavenging activity. All the results implicated that, the hawthorn fruit may has the available potential to be utilize as a potential source of natural antioxidant.

• 생명과학회지
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• 제8권5호
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• pp.473-478
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• 1998

본 연구에서는 된장의 기능성 중에서 대두, 메주, 된장의 발효과정을 거치는 동안 항산화 효과와 아질산염 소거 효과의 변화 추이를 고찰하기 위해 DPPH radical 소거능과 아질산염 소거능을 통한 된장의 활성을 시험하였다. 대두, 메주 및 된장은 methanol extract를 계통 추출법에 의거하여 여러 가지 용매를 사용하여 분획하였으며, 각 분획에 대한 활성실험을 행하였다. MeOH ext.로 본 항산화 효과는 된장〉메주〉대두의 순으로 나타났고, 메주와 대두 간의 효과의 차는 거의 없었다. 아질산염 소거효과는 된장〉대두〉메주의 순을 보였고, 래디칼 소거능보다 아질산염 소거능이 더 효과적으로 나타났다. Ethyl acetate 분획에 있어 항산화 효과가 켰으며, 특히 아질산염의 소거 효과는 L-ascorbic acid의 효과를 상회하였다.이에 된장의 ethyl acetate 분획을 반복 정제하여 genis-tin, daidzein, genistein을 분리하였으며, 이는 된장의 항산화 및 아질산염 소거 효과를 나타내는 활성 물질들은 대두중의 페놀성물질에서 크게 벗어나지 않음을 알 수 있었다.

• 한국응용과학기술학회지
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• 제32권2호
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• pp.312-319
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• 2015

In this study, n-butanol and ethyl acetate extracts were prepared from raw yam (Dioscorea batatas DECNE.). Their antioxidative potencies were investigated employing various in vitro methods, such as ferrous ion chelating, ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging and nitrite scavenging activity. The n-butanol fraction was assayed to possess stronger antioxidant activity by ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and NO radical scavenging activity. However, ethyl acetate extract was more effective in chelating ferrous ion and scavenging nitrite. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

• 한국약용작물학회지
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• 제21권4호
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• pp.255-261
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• 2013

The purpose of this study was to examined the antioxidant activities by water and 70% ethanol extract from durian (Durio zibethinus.) seed, sarcocarp and peel. Durian extract were studied for reducing sugar content, polyphenol content, superoxide dismutase (SOD) like activity, electron donating ability, nitrite scavenging ability, flavonoid content, hydroxy radical scavenging activity. Reducing sugar content were increased peel > sarcocarp > seed. Total polyphenol, flavonoid content, DPPH radical scavenging ability and SOD like activity were increased seed > peel > sarcocarp. Total polyphenol content was relatively high as $21.90{\pm}0.50mg/g$ in the ethanol extract of the seed. DPPH radical scavenging ability was relatively high as $62.08{\pm}2.63%$ in the water extract of the seed. Nitrite scavenging ability was no significant difference. Hydroxy radical scavenging activity was increased seed > peel > sarcocarp, was relatively high as $58.27{\pm}1.13%$ in the water extract of the seed.

• Fisheries and Aquatic Sciences
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• 제4권1호
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• pp.47-49
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• 2001

Nitrite scavenging activity of a methanol extract of Symphyocladia latiuscula was studied. The methanol extract scavenged the nitrite in a dose-dependent manner. The MeOH extract was then sequentially partitioned with n-hexane, $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$. The scavenging activity of the fractions increased in order of $CH_2Cl_2$, n-hexane, EtOAc, n-BuOH, and $H_2O$. Especially, the activity of the $CH_2Cl_2$ fraction was comparable to that of L-ascorbic acid. Column chromatography of the most active $CH_2Cl_2$ fraction over silica gel yielded three active bromophenol congeners (1-3) which were identified as (2R)-2-(2,3,6-tribromo 4,5-dihydro­xybenzyl) cyclohexanone (1), 2,3,6-tribromo 4,5-dihydroxybenzyl methyl ether (2), and 2,3,6­tribromo 4,5-dihydroxybenzyl alcohol (3) respectively.

• 동의생리병리학회지
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• 제21권4호
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• pp.955-960
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• 2007

Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Acanthopanax sessiliflorus was investigated. Electron-donating ability of extract at $RC_{50}$ was 29.93 ${\mu}g/mL$. After addition of 0.96 mg/mL extract, autooxidation of pyrogallol decreased to 22.85% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 24, 48, 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased by 48.89%, 45.0%, 46.34% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed $410.25{\pm}4.74$ GAE ${\mu}g/mg$ of extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 88.3% by addition of 0.2 mg/mL. These results suggest that methanol extract from A. sessiliflorus can be used as bioactive and functional material.

• 동의생리병리학회지
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• 제21권3호
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• pp.666-670
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• 2007

Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Ricinus communis. was investigated. Electron-donating ability of extract at RC$_{50}$ was 114.02 ${\mu}g$/mL. After addition of 0.46 mg/mL extract, autoxidation of pyrogallol decreased to 32.99% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 24, 48, 96 hours at 40$^{\circ}C$, lipid peroxidation values significantly decreased by 85.50%, 87.77%, 90.95% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed 83.98 ${\pm}$ 5.66 GAE ${\mu}g$/mg of extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, decreasing to 47.24% by addition of 0.2 mg/mL. These results suggest that methanol extract from Ricinus communis. can be used as bioactive and functional material.

• 한국식품영양학회지
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• 제11권4호
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• pp.426-430
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• 1998

This study was conducted to investigate electron donating ability, antioxdative activity and nitrite-scavenging effect of solvent extracts from Gyrophora esculenta. The phenolic compounds of diethylether, butanol, petroleum ether and hexane extracts were 194.20$\mu\textrm{g}$/ml, 98.80$\mu\textrm{g}$/ml, 1.60$\mu\textrm{g}$/ml and 0.20$\mu\textrm{g}$/ml, respectively. Electron donating abilities of diethyleter and butanol extracts from Gyrophora esculaenta were 80.79% and 72.81%, respectively. Absorption of DPPH by diethyleter and butanol extracts sharply increaed at initial stage. Antioxidative activities of fiethylether and butanol extracts by peroxide values are rather high as compared to those of control, but low s compared to those of BHA. Nitrite-scavenging abilities of diethylether, butanol and petroleum ether from Gyrophora esculenta were 96.07%, 77.39% and 15.07%, respectively.

• 한국자원식물학회지
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• 제26권4호
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• pp.426-432
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• 2013

본 연구에서는 미성숙 목화다래 추출물의 항산화능을 알아보기 위해 다래를 크기별로 구분하여 총 폴리페놀 및 플라보노이드 함량과 DPPH radical의 소거능, 아질산염 소거능, ABTS radical 소거능 및 SOD 유사활성을 측정하였다. 추출물의 페놀성 화합물과 플라보노이드 함량은 목화다래의 크기가 작을수록 높게 나타났으며, DPPH radical 소거능 및 ABTS radical의 소거능 또한 작은 크기에서 상대적으로 높은 활성을 보였다. 이는 다른 선행연구들에서와 같이 페놀함량이 높은 실험군에서 DPPH radical 소거능과 ABTS radical 소거능이 높게 나타나 유의성을 확인하였다. 아질산염 소거능은 인체 위 내부환경과 같은 pH 1.2에서는 높은 활성을 보이다가 pH 4.2에서는 점차로 활성이 떨어지는 결과를 보였으며, 중성에 가까운 pH 6.0에서는 아질산염 소거에 영향을 미치지 못하는 것으로 확인되었다. 또한 SOD 유사활성은 대조군인 ascorbic acid에 비하면 낮은 활성이었지만, 기 보고된 다른 천연물들의 효소활성과 비교하였을 때 더 높거나 비슷한 결과를 나타냈다. 이와 같이 본 실험을 통해 미성숙 목화다래의 뛰어난 항산화 활성을 확인할 수 있었으며, 이는 향후 화장품이나 기능성 식품 등에 있어서 새로운 소재개발의 가능성을 시사해주는 것으로서, 그동안 주로 천연 솜의 생산을 위해 재배되어왔던 목화의 제한적 활용성을 더욱 다양하게 해줄 것으로 기대된다.

• 한국식품영양학회지
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• 제35권5호
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.