Browse > Article
http://dx.doi.org/10.7732/kjpr.2013.26.4.426

Evaluations on Antioxidant Effect of Methanol Extract from Immature Cotton Boll  

Park, Hee-Jeong (Interdisciplinary Program of Perfume and Cosmetics, Chonnam National University)
Lee, Ki-Young (Faculty of Applied Chemical Engineering, Chonnam National University)
Publication Information
Korean Journal of Plant Resources / v.26, no.4, 2013 , pp. 426-432 More about this Journal
Abstract
The results of the content of total polyphenol and flavonoid, DPPH (1-1-diphenyl-2-picryl-hydrazyl) radical scavenging activity, ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging activity, nitrite scavenging activity and SOD-like activity of methanol extracts from immature cotton boll are follows. The contents of total polyphenol and flavonoid compound were higher in small size cotton boll, and DPPH and ABTS radical scavenging activity also showed a relatively high activity in the small size. These results indicate that there is a correlation between phenol content and DPPH radical scavenging, ABTS radical scavenging. The test concentrations of immature cotton boll extract for measuring DPPH and ABTS radical scavenging activities were set as 1.25, 2.5, 5, 10 and 20 mg/ml. Immature cotton boll has high radical scavenging activity at the concentration of 1.25~20 mg/ml and the result showed a tendency to increase in a concentration-dependent. The nitrite scavenging activity showed high activity in the pH 1.2, and the result in the pH 4.2 showed progressively less active, and in the pH 6.0 near neutral was confirmed that does not affect the nitrite scavenging. In addition, SOD-like activity showed somewhat lower activity compared with ascorbic acid, but tended to be higher when compared with the results of the other natural substances. Through this experiment, we confirmed that immature cotton boll was excellent antioxidant activity. Therefore, it is demonstrated that the cotton suggest the possibility of development of new material for cosmetic product or functional food in the future, and is expected to make a greater usability.
Keywords
Polyphenol; Flavonoid; DPPH radical scavenging; ABTS radical scavenging; Nitrite scavenging activity; SOD-like activity;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Ko, K.S. 2012. A study on antioxidant effect of methanol extract from Viola mandshurica. J. Kor. Soc. Cosm. 18(5):1082-1086 (in Korean).
2 An, B.J. and J.T. Lee. 2002. Studies on biological activity from extract of Crataegi Fructus. J. Kor. Herbology 17:29-38 (in Korean).   과학기술학회마을
3 Bae, J.S. and T.H. Kim. 2009. Acetylcholinesterase inhibitory and antioxidant properties of Aster yomena extract. Kor. J. Herbology 24(4):121-126 (in Korean).   과학기술학회마을
4 Bae, K.H. 2000. The Medical Plants of Korea. Kyo-Hak publishing Co., Seoul, Korea. p. 332 (in Korean).
5 Miles, D. H., V. Chittawong, A.M. Payne, P.A. Hedin and U. Kokpol. 1990. Cotton boll weevil antifeedant activity and antifungal activity (Rhizoctonia solani and Pythium ultimum) of extracts of the stems of Wedelia biflora. J. Agric. Chem. 38(7):1591-1594.
6 Nivea, M.M.I., A.R. Sampietro and M.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethropharmacol. 71: 109-114.   DOI   ScienceOn
7 Prior, R.L., X. Wu and K. Schaich. 2005. Standardized method for the determination of antioxidant capacity and phenolics in foods and dietary supplement. J. Agric. Food Chem. 48:115-119.
8 Re, R., N. Pellegrini, A. Proteggente, M. Yang and R.E. Catherine. 1999. Antioxidant activity applying an improved ABTS radical cation decolorzation assay. Free Radical Biol. Med. 26:1231-1237.   DOI   ScienceOn
9 Rhim, T.J., M.Y. Choi and H.J. Park. 2012. Antioxidative activity of Rumex cripus L. extract. Korean J. Plant Res. 25(5):568-577 (in Korean).   DOI   ScienceOn
10 Sen, T., H.S.H. Nasralla and A.K.N. Chaudhuri. 1995. Studies on the antiinflammatory and related pharmacological activities of Psidium guajava: A preliminary report. Phytotherapy Research 9(2):118-122.   DOI   ScienceOn
11 Swain, T., W.E. Hillis and M. Ortega. 1959. Phenolic constituents of ptunus domeatica, I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10:83-88.
12 Kim, Y. H., C. E. Lee and B. S. Kim. 2011. Study on cytotoxicity test and anti-oxidant activity of herb complex (Phellinus Linteus, Glycyrrhiza uralensis Fischer and Centella asiatica. J. Kor. Soc. Cosm. 17(3): 441-446 (in Korean).
13 Kim, E.D. 1962. Agricultural Dictionary. Hak-Won Publishing Co., Seoul, Korea. p. 586 (in Korean).
14 Kim, J.W. 1998. Antitumor agents isolated from cotton balls. Ministry of Science and Technology. Gwacheon, Korea. pp. 5-17 (in Korean).
15 Kim, Y.E., J.W. Yang, C.H. Lee and E.K. Kwon. 2009. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake sing (Pine Mushroom). J. Korean. Soc. Food. Sci. Nutr. 38(5):555-560 (in Korean).   과학기술학회마을   DOI   ScienceOn
16 Kuhnau, J. 1976. The Flavonoids: a class of semiessential food components: their role in human nutrition. World Rev. Nutr. Diet. 24: 117-120.
17 Kwak, C.S., S.A. Kim and M.S. Lee. 2005. The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. Korean. J. Soc Food Sci Nutr. 34(8):1143-1150 (in Korean).   과학기술학회마을   DOI   ScienceOn
18 Lee, D.S., M.S. Lim, S.S. Kwan, S.Y. Kim and S.N. Park. 2012. Antioxidative activity and componential analysis of Chamaecyparis obtuse leaf extract. J. Appl. Chem. 23(1):93-99 (in Korean).
19 Lim, T.S., J.R. Do, O.J. Kwon and H.K. Kim. 2007. Physiological activities of Agaricus Bisporus extracts as affected by solvents. J. Korean Soc. Food. Sci. Nutr. 36:383-388 (in Korean).   과학기술학회마을   DOI   ScienceOn
20 Lee, S.Y., J.H. An, H. Chun and H.Y. Cho. 2003. Isolation and characterzation of MMP-1 inhibitor peptide from crataegus pinnatifida bunge in fibroblast cell line HS 68 cells. Korean. J. Soc. Agric. Chem. Biotechnol. 46(1):60-65 (in Korean).
21 Middleton, E. and C. Kandaswami. 1994. Potential healthpromoting properties of citrus flavonoids. Food Tech. 48: 115-119.
22 Bryan, D.M., J. Murnaghan, K.S. Jones and S.R. Bowley. 2000. Iron superoxide dismutase expression in transgenic alfalfa increase winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiol. 122:1427-1438.   DOI   ScienceOn
23 Chance, B., H. Sies and A. Boveris. 1979. Hydroperoxide metabolism in mammalian organs. Physiol. Rev. 59:527-605.   DOI
24 Davies, K.J.A. 1995. Oxidative stress: The paradox of aerobic life. In Free Radicals and Oxidative Stress: Environment, Drugs and Food Additives, Rice-Evans C., B. Halliwell and G. Lunt (eds.), Biochemical Society Symposium. Portland Press, London, UK. pp. 1-31.
25 Donnelly, J.K., K.M. McLellan, J.K. Walker and D.S. Robinson. 1989. Superoxide dismutase in foods. A Review. Food Chem. 33:243-270.   DOI   ScienceOn
26 Gray, J.I. and L.R. Dugan. 1975. Inhibition of N-nitrosamine formation in model food system. J. Food Sci. 40:981-984.   DOI
27 Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1204.   DOI   ScienceOn
28 Hwang, Y.H., D.H. Kim, H.J. Kim, J.Y. Hwang, T.S. Park, I.S. Lee and J.H. Son. 2011. Antioxidant activities and nitric oxide production of medicine plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia lactiflora) J. Appl. Biol. Chem. 54(3): 171-177 (in Korean).   과학기술학회마을   DOI   ScienceOn
29 Jung, W.Y and J.M. Jeong. 2012. Change of antioxidative activity at different harvest time and improvement of atopic dermatitis effects for persimmon leaf extract. Kor. J. Herbology 27(1):41-49.   과학기술학회마을   DOI   ScienceOn
30 Kato, H., I.E. Lee, N.V. Chuyen, S.B. Kim and F. Hayase. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidines. Agr. Biol. Chem. Tokyo. 51: 1333-1338.   DOI
31 Kawaguchi, K., T. Mizuno, K. Aida and K. Uchino. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and pseudomonas. Biosci Biotecnol Biochem. 61:102-104.   DOI   ScienceOn