Antioxidative Activity and Nitrite Scavenging Ability of Ethyl Acetate Extract from Acanthopanax sessiliflorus

오가피 메탄올 추출물의 항산화 효과 및 아질산염 소거작용

  • Lim, Jin-A (Department of Herbal Resources, professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Yun, Bo-Won (Department of Herbal Resources, professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Jeong-Il (Department of Physical Therapy, College of Complementary and Alternative Medicine, Jeonju University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, professional Graduate School of Oriental Medicine, Wonkwang University)
  • 임진아 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 윤보원 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 강정일 (전주대학교 대체의학대학 물리치료학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2007.08.25

Abstract

Efficacy of antioxidative activity and nitrite scavenging ability of methanol extract from Acanthopanax sessiliflorus was investigated. Electron-donating ability of extract at $RC_{50}$ was 29.93 ${\mu}g/mL$. After addition of 0.96 mg/mL extract, autooxidation of pyrogallol decreased to 22.85% by superoxide dismutase-like activity. In antioxidative activity of extract against linoleic acid during incubation times of 24, 48, 96 hours at $40^{\circ}C$, lipid peroxidation values significantly decreased by 48.89%, 45.0%, 46.34% with addition of 0.2 mg/mL, respectively. Total phenolic content was determined as gallic acid equivalents (GAE) and values revealed $410.25{\pm}4.74$ GAE ${\mu}g/mg$ of extract. Nitrite scavenging ability showed the most remarkable effect at pH 1.2, exhibited to 88.3% by addition of 0.2 mg/mL. These results suggest that methanol extract from A. sessiliflorus can be used as bioactive and functional material.

Keywords

References

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