• Animal cells and systems
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• 제3권1호
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• pp.69-72
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• 1999

A tandemly repeated DNA sequence was identified and characterized y the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5'-GAAACGGGTG-3', in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C-banded constitutive heterochromatin in the terminal regions of both arms of all 6 chromosomes.

• 한국수산과학회지
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• 제27권5호
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• pp.613-619
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• 1994

황해에 서식하는 참조기(Pseudosciaena polyactis Bleeker) 각 계군의 유전적 차이점을 분석하기 위하여 중국에서 3지역(Zhoushan, Shanghai, Qingdao), 한국 2지역(목포, 인천)에서 채집된 참조기로부터 mitochondrial DNA(mtDNA)의 RFLP(제한효소 절편 다형현상)를 분석하였다. 총 18종의 제한효소를 이용하여 처리한 결과 5개 집단 모두 동일한 크기인 $16.9{\pm}0.6kb$의 mtDNA를 소유한 것으로 나타났으며 이는 다른 어류군들과 유사한 크기였다. 참조기 mtDNA에 대한 RFLP 분석을 행한 결과 각 집단 마다 대략 40여개의 절편이 관찰되었고 5개 집단 모두 동일한 mtDNA 절편양상을 보였으나 사용된 제한효소 중 좌ApaI, EcoRI, PstI, SstII 및 SmalI에서 중국과 한국 집단내 또는 집단간 절편 양상의 차이도 관찰할 수 있었다.

• BMB Reports
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• 제28권3호
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• pp.249-253
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• 1995

The nonenzymatic glycation of copper, zinc-superoxide dismutase (Cu,Zn-SOD) led to inactivation and fragmentation of the enzyme. The glycated Cu,zn-SOD was isolated by boronate affinity chromatography. The formation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in calf thymus DNA and the generation of strand breaks in pBhiescript plasmid DNA by a metal-catalyzed oxidation (MCO) system composed of $Fe^{3+}$, $O_2$, and glutathione (GSH) as an electron donor was enhanced more effectively by the glycated CU,Zn-SOD than by the nonglycated enzyme. The capacity of glycated Cu,Zn-SOD to enhance damage to DNA was inhibited by diethylenetriaminepentaacetic acid (DETAPAC), azide, mannitol, and catalase. These results indicated that incubation of glycated CU,Zn-SOD with GSH-MCO may result in a release of $Cu^{2+}$ from the enzyme. The released $Cu^{2+}$ then likely participated in a Fenton-type reaction to produce hydroxyl radicals, which may cause the enhancement of DNA damage.

• 통합자연과학논문집
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• 제1권3호
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• pp.230-233
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• 2008

A simple and rapid method is described for extending the 5'- or 3'-end genomic sequence of a known partial sequence by only a single round of PCR. This method involves digesting and ligating genomic and plasmid DNAs, and amplifying the 5'-upstream or 3'-end downstream sequence of the known DNA sequence, using two primers, one gene specific and the other plasmid specific. A single round of PCR amplification is sufficient to produce gene-specific bands detectable in gels. By using this approach, 5'-end genomic sequence of the D-amoeba sams gene was extended.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.353-353
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• 2006

Deoxyribonucelic acid (DNA) molecules have a huge molecular weight so that DNA was reported to be a promising natural polymer to give durable films. Among many applications of DNA, the authors focused their attention on separation membranes derived from DNA because membranes will play an important role in environmental and energy related processes. DNA-polyion complex membranes were prepared from DNA and corresponding polycations. The DNA-polyion complex membranes showed chiral separation ability toward racemic amino acid mixtures.

• 생약학회지
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• 제49권2호
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• pp.145-154
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• 2018

Aconiti Kusnezoffii Tuber has been traditionally used to treat the symptoms of rheumatoid arthritis and joint pain. The main constituents are diterpenoid alkaloids such as benzoylmesaconine, benzoylaconine, mesaconitine, aconitine, and hypaconitine. In Korea, Aconiti Kusnezoffii Tuber is officially defined as the tubers of Aconitum kusnezoffii Reichb., A. ciliare Decasisne, and A. triphyllum Nakai. On the other hand, only the tuber of A. kusnezoffii is to be used in China. In order to identify the botanical origin of Aconiti Kusnezoffii Tuber circulated in Korea, we analyzed 24 samples of Aconiti Kusnezoffii Tuber obtained from local markets for comparative DNA analysis. The sequence analysis of nrRNA ITS 1 was useful to distinguish Aconitum species and revealed that the roots of A. karakolicum were circulated in Korean markets without discretion. HPLC quantitative analysis showed that aconitine was detected at the highest amount in A. karakolicum. Authentic diterpenoid alkaloids were coinjected for quantification of aconitine-type ingredients. All data were statistically grouped by Principal Component Analysis (PCA). This study suggests that both molecular and chemical analyses should be utilized for the standardization and the quality control for Aconiti Kusnezoffii Tuber.

• 환경생물
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• 제18권1호
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• pp.53-61
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• 2000

서울시 소재 지하수 중 중금속으로 오염되어 음용수 이외의 생활 용수로 사용하고 있는 1개 정점과 음용수로 사용하고 있는 1개 정점, 대조군으로 강화도 천연 동굴의 1개 정점을 대상으로 실험을 실시하였다. 지하수 세균군집의 유전적 다양성의 변화를 보기 위해 지하수 세균 군집에서 16SrDNA를 증폭하는 primer로 PCR(polymerase chain reaction)을 실시한 후 ARDRA(amplified ribosomal DNA restriction analysis) 지문 분석으로 비교하였다. 16S rDNA를 증폭하여 제한효소 지문분석을 한 결과 in situ와 음용수에서 유전적 다양성이 상대적으로 크게 나타났다. 지하수 세균 군집의 ARDRA지문 분석은 상이한 환경과 서식지를 반영한 유전적 차이를 빠르게 비교 분석할 수 있었으며 지하수의 오염도에 따른 미생물의 다양성(천연 동굴>음용수>오염수)을 확인할 수 있었다.

• BMB Reports
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• 제52권11호
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• pp.635-640
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• 2019

CpG-DNA triggers the proliferation and differentiation of B cells which results in the increased production of antibodies. The presence of bacteria-reactive IgM in normal serum was reported; however, the relevance of CpG-DNA with the production of bacteria-reactive IgM has not been investigated. Here, we proved the function of CpG-DNA for the production of bacteria-reactive IgM. CpG-DNA administration led to increased production of bacteria-reactive IgM both in the peritoneal fluid and serum through TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM was reproduced in vitro. We established a bacteria-reactive monoclonal IgM antibody using CpG-DNA stimulated-peritoneal B cells. The monoclonal IgM antibody enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM. We also suggest the possible application of the antibodies for the treatment of antibiotics-resistant bacterial infections.

• 한국산림과학회지
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• 제106권4호
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• pp.408-416
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• 2017

본 연구는 국내 천연기념물 은행나무 23개체를 대상으로 8개의 microsatellite 마커를 이용하여 DNA 지문분석을 수행하였다. 그 결과, 평균 대립유전자수는 6.875개, 평균 이형접합도 관찰치와 기대치는 각각 0.711과 0.710로 나타났다. 이러한 수치는 동일한 마커로 일본 및 중국 은행나무에서 분석된 기존 연구 결과와 유사하였다. 분석된 8개 마커의 다형성 정보량(PIC) 값 및 개체식별력(PD)은 각각 0.677과 0.9999로 높은 개체식별 효율을 나타내었다. 실제 유전형을 비교한 결과에서도 모든 천연기념물 은행나무와 추가로 분석된 일반 은행나무 13개체가 모두 식별되었다. 천연기념물 은행나무에서 계산된 PIC 값을 기준으로 상위 3개의 마커(Ging06, Gb60, Gb61)에서 계산된 개체인식력과 개체식별력이 각각 $8.045{\times}10^{-5}$ 및 99.99%로 계산되므로, 이들 3개의 마커가 은행나무 개체식별을 위한 DNA 지문 분석에 우선 적용이 가능할 것이다. 본 연구의 DNA 지문 분석 결과는 천연기념물 은행나무의 관리와 후계목 육성 및 은행나무 선발 개체의 유전적 동일성 검정에 유용한 자료로 활용될 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.93-97
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• 2003

We describe here the cloning of a cDNA encoding putative calreticulin (CRT) from the silkworm, Bombyx mori. The CRT cDNA comprised of 1,194 bp encoding 398 amino acid residues. B. mori. CRT has a HDEL sequence at the end of the C-domain. The B. morl, CRT showed 88% protein sequence identity to the G. mellonella CRT, 71 % to A. aegypti CRT, and 63% to H. sapiens CRT, Phylogenetic analysis revealed that the deduced amino acid sequences of the B. mori CRT formed a highly inclusive subgroup with other insect CRTs. Northern blot analysis exhibited an expression of the B. mori CRT gene in the fat body, evidencing the fat body as a major site for CRT synthesis.