[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2019.52.11.018

CpG-DNA induces bacteria-reactive IgM enhancing phagocytic activity against Staphylococcus aureus infection

Kim, Te Ha (Department of Microbiology, College of Medicine, Hallym University)
Kim, Dongbum (Center for Medical Science Research, College of Medicine, Hallym University)
Lee, Heesu (Department of Microbiology, College of Medicine, Hallym University)
Kwak, Min Hyung (Department of Microbiology, College of Medicine, Hallym University)
Park, Sangkyu (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Lee, Younghee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University)
Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)

Publication Information

BMB Reports / v.52, no.11, 2019 , pp. 635-640 More about this Journal

Abstract

CpG-DNA triggers the proliferation and differentiation of B cells which results in the increased production of antibodies. The presence of bacteria-reactive IgM in normal serum was reported; however, the relevance of CpG-DNA with the production of bacteria-reactive IgM has not been investigated. Here, we proved the function of CpG-DNA for the production of bacteria-reactive IgM. CpG-DNA administration led to increased production of bacteria-reactive IgM both in the peritoneal fluid and serum through TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM was reproduced in vitro. We established a bacteria-reactive monoclonal IgM antibody using CpG-DNA stimulated-peritoneal B cells. The monoclonal IgM antibody enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM. We also suggest the possible application of the antibodies for the treatment of antibiotics-resistant bacterial infections.

Keywords

CpG-DNA; IgM; Phagocytosis; Staphylococcus aureus; TLR9;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)

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