International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Cloning of a cDNA Encoding Putative Calreticulin from the Silkworm, Bombyx mori

  • Kim, Seong-Ryul (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Kang, Seok-Woo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Nho, Si-Kab (College of Agriculture and Life science, Kyungpook National University) ;
  • Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2003.03.01
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Abstract

We describe here the cloning of a cDNA encoding putative calreticulin (CRT) from the silkworm, Bombyx mori. The CRT cDNA comprised of 1,194 bp encoding 398 amino acid residues. B. mori. CRT has a HDEL sequence at the end of the C-domain. The B. morl, CRT showed 88% protein sequence identity to the G. mellonella CRT, 71 % to A. aegypti CRT, and 63% to H. sapiens CRT, Phylogenetic analysis revealed that the deduced amino acid sequences of the B. mori CRT formed a highly inclusive subgroup with other insect CRTs. Northern blot analysis exhibited an expression of the B. mori CRT gene in the fat body, evidencing the fat body as a major site for CRT synthesis.

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