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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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CpG-DNA induces bacteria-reactive IgM enhancing phagocytic activity against Staphylococcus aureus infection

  • Kim, Te Ha (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kim, Dongbum (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Lee, Heesu (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kwak, Min Hyung (Department of Microbiology, College of Medicine, Hallym University) ;
  • Park, Sangkyu (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Younghee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
  • Received : 2019.01.14
  • Accepted : 2019.03.13
  • Published : 2019.11.30
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Abstract

CpG-DNA triggers the proliferation and differentiation of B cells which results in the increased production of antibodies. The presence of bacteria-reactive IgM in normal serum was reported; however, the relevance of CpG-DNA with the production of bacteria-reactive IgM has not been investigated. Here, we proved the function of CpG-DNA for the production of bacteria-reactive IgM. CpG-DNA administration led to increased production of bacteria-reactive IgM both in the peritoneal fluid and serum through TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM was reproduced in vitro. We established a bacteria-reactive monoclonal IgM antibody using CpG-DNA stimulated-peritoneal B cells. The monoclonal IgM antibody enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM. We also suggest the possible application of the antibodies for the treatment of antibiotics-resistant bacterial infections.

Keywords

References

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