Browse > Article
http://dx.doi.org/10.14578/jkfs.2017.106.4.408

DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers  

Lee, Jei-Wan (Division of Forest Genetic Resources, National Institute of Forest Science)
Lee, Min-Woo (Division of Forest Genetic Resources, National Institute of Forest Science)
Ahn, Ji-Young (Division of Forest Genetic Resources, National Institute of Forest Science)
Hong, Kyung-Nak (Division of Forest Genetic Resources, National Institute of Forest Science)
Baek, Seung-Hoon (Division of Forest Genetic Resources, National Institute of Forest Science)
Kim, Sang-Chul (Division of Forest Genetic Resources, National Institute of Forest Science)
Publication Information
Journal of Korean Society of Forest Science / v.106, no.4, 2017 , pp. 408-416 More about this Journal
Abstract
This study describes DNA fingerprinting analysis of twenty-three natural monument individuals of Ginkgo biloba using eight microsatellite markers. The average number of observed alleles was 6.875, and the expected heterozygosity and the observed heterozygosity were 0.711 and 0.710, respectively. This results were similar to those of the previous studies on Ginkgo trees analyzed by same markers in China and Japan. PIC value and PD were calculated at 0.677 and 0.9999 respectively, indicating a high individual identification efficiency. In fact, all of the natural monument ginkgo trees and additionally analyzed thirteen general ginkgo tress were identified by genotype comparison. PI and PD calculated in three markers (Ging06, Gb60, Gb61) with the highest PIC values calculated in natural monument ginkgo trees were $8.045{\times}10^{-5}$ and 99.99%, respectively. Thus, these three markers could be preferentially used in DNA fingerprinting for identifying ginkgo tree individuals. The results in this study will be useful for management of natural monument ginkgo trees, proliferation of their progeny and genetic identification of individuals selected in breeding process.
Keywords
Ginkgo biloba; natural monument; microsatellite marker; power of discrimination; DNA fingerprinting;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Yoo, M.H., Kim, Y.S., Rho, J.R. and Kim, J.T. 2008. Phylogenetic study of the Korean goral by mitochondrial DNA cytochrome b gene sequence analysis. Laboratory Animal Research 24: 33-37.
2 Waits, L.P., Luikart, G. and Taberlet, P. 2001. Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Molecular Ecology 10: 249-256.   DOI
3 Zhao, Y., Paule, J., Fu, C. and Koch, M.A. 2010. Out of China: distribution history of Gingko biloba L. TAXON 59(2): 495-504.
4 Echenard, V., Lefort, F., Calmin, G., Perroulaz, R. and Belhahri, L. 2008. A new and improved automated technology for early sex determination of Gingo biloba. Arboriculture and Urban Forestry 34(5): 300-307.
5 Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314.
6 Chang, C.S., Kim, H. and Chang, K.S. 2011. Illustrated Encyclopedia of Fauna and Flora of Korea. 43. Woody plants. Design Post. Gyeonggido. Korea. pp. 511.
7 Cho, B.W., Jung, J.H., Kim, S.U., Kim, H.S., Lee, H.G., Jo, G.J. and Song, K.D. 2007. Establishment of genetic characteristics and individual identification system using microsatellite loci in Jeju native horse. Jounal of Life Science 17: 1441-1446.   DOI
8 Go, G.H. 2010. Our trees that we should protect. 3. Ginkgo trees. Dasan Books. Seoul, Korea. pp. 161.
9 Gong, W., Zeng, Z., Chen, Y.Y., Chen, C., Qiu, Y.X. and Fu, C.X. 2008. Glacial refugia of Gingko biloba and human impact on its genetic diversity: evidence from chloroplast DNA. Journal of Integrative Plant Biology 50(3): 368-374.   DOI
10 Hildebrand, W.H., Madrigal, J.A., Little, A.M. and Parham, P. 1992. HLA-Bw22: a family of moleculues with identity to HLA-B7 in the alpha 1-helix. Journal of Immunology 148(4): 1155-1162.
11 Hong, Y.P., Cho, K.J., Hong, K.N. and Shin, E.M. 2001. Diversity of I-SSR variants in Gingko biloba L. planted in 6 regions of Korea. Journal of Korean Forest Society 90(2): 169-175.
12 Kang, K.H., Chong, Y.J. and Kim, H.N. 1999. The genetic relationship of Zelkova serrata registered the natural monument using RAPD markers. Korean Journal of Environmental Biology 17(1): 89-94.
13 Hong, Y.P., Kim, Y.M., Ahn, J.Y., Yang, B.H. and Hur, S.D. 2009. DNA fingerprinting of old big trees of natural monument. pp. 230-231 In: Korean Forest Society. Proceedings of Korean Forest Society. Dongguk University. Seoul, Republic of Korea.
14 Jacobs, B.P. and Browner, W.S. 2000. Ginkgo biloba: a living fossil. The American Journal of Medicine 108: 341-342.   DOI
15 Kalia, R.K., Rai, M.K., Kalia, S., Singh, R. and Dhawan, A.K. 2010. Microsatellite markers: an overview of the recent progress in plants. Euphytica 177: 309-334.
16 Kim, Y.M., Hong, K.N., Park, Y.J., Hong, Y. P. and Park, J.I. 2015. Estimating the parameters of pollen flow and mating system in Pinus densiflora Population in Buan, South Korea, using Microsatellite Markers. Korean Journal of Plant Resources 28(1): 101-110   DOI
17 Kim, H.J. 2011. Growth conditions of natural monument old big trees in Gyeongsangnamdo, Korea. Journal of Korean Environmental Restoration Technology 14: 103-112.
18 Kim, H.J. 2012a. The status and the characteristic of natural resources in UNESCO sacred natural site, South Korea. Journal of Korea Contents Association 12: 492-501.   DOI
19 Kim, H.J. 2012b. Soil status and management of natural monument plants: focusing on the big old tree of Chungnambuk. Journal of Korean Institute of Traditional Landscape Architecture 10: 41-50.
20 KCHA (Korea Cultural Heritage Administration). 2011. A study on genetic diversity in Korean natural monuments long-tailed goral (Naemorhedus caudatus). pp. 30.
21 KCHA (Korea Cultural Heritage Administration). 2015. A study on nurturing progeny of Korean natural monuments big old trees. pp. 246.
22 Liu, K. and Muse, S.V. 2005. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21(9): 2128-2129.   DOI
23 Lee, J.H., Han, S.Y., Choi, K. and Cho, H.J. 2000. Measuring preservation value of oriental arber forest in Taegu City. Journal of Forest Recreation 4: 55-66.
24 Lee, J.W., Baek, S.H., Hong, K.N., Hong, Y.P., Lee, S.W. and Ahn, J.Y. 2015. Development and characterization of chloroplast simple sequence repeat markers in Pinus koraiensis. Journal of Korean Forest Society 104(4): 549-557.   DOI
25 Lee, K.J. 2006. Natural monument in Korea. Big old tress. Academy. Seoul, Korea. pp. 390.
26 Lee, K.M., Kim, Y.Y. and Chung, J.M. 2009. Genetic diversity of natural populations of Populus maximowiczii Henry in Korea based on microsatellite marker analysis. Proceeding of Journal of Korea Forest Society pp. 259-260.
27 Lee, K.M., Kim, Y.Y. and Jung, O.H. 2011. Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis. Genes and Genomics 33: 163-171.   DOI
28 Nybom, H. 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in Plants. Molecular Ecology 13: 1143-1155.   DOI
29 Park, S.J. 2009. Exploration of cultural trees in Korea. Wangeuiseojae. Seoul, pp. 411.
30 Park, W.K., Oh, J.A., Kim, Y.J., Kim, S.K., Park, S.Y., Son, B.H. and Choi, S.I. 2010. Species of wooden buddhist statues of the late Joseon dynasty in Jeollado. South Korea. Korea Furniture Society 21(1): 72-82.
31 KCHA (Korea Cultural Heritage Administration). 2016. A study on drawing up measures for conservation and management of Korean natural monuments fauna species. pp. 238.
32 Soh, W.Y. and Yoon, S. 2011. Science, culture and mystery of ginkgo. S-wave Science. Seoul, Republic of Korea. pp. 204.
33 Peakall, R. and Smouse, P.E. 2006. GeneAlex 6: genetic analysis in Excel. population genetic software for teaching and research. Molecular. Ecology Notes 6: 288-295.   DOI
34 Perrier, X., Flori, A. and Bonnot, F. 2003. Data analysis methods. pp. 43-76. In: Hamon P., Seguin, M., Perrier, X. and Glaszmann, J.C. (ed). Genetic diversity of cultivated tropical plants. Enfield Science Publishers. Montpellier. France.
35 Shen, L., Chen, X.Y., Zhang, X., Li, Y.Y., Fu, C.X. and Qiu, Y.X. 2005. Genetic variation of Ginkgo biloba L. (Ginkgoaceae) based on cpDNA PCR-RFLPs: inference of glacial refugia. Heredity 94: 396-401.   DOI
36 Son, J.W., Shin, J.H. and Lee, J.J. 2016. An analysis on vitality status of big old trees preserved as natural monuments based on artificial management. Korean Journal of Environment and Ecology 30(3): 415-422.   DOI
37 Varshney, R.K., Graner, A. and Sorrells, M.E. 2005. Genic microsatellite markers in plants: features and applications. TRENDS in Biotechnology 23: 48-55.   DOI
38 Yan, X.F., Lian, C.L. and Hogetsu, T. 2006. Development of microsatellite markers in ginkgo (Ginkgo biloba L.). Molecular Ecology Notes 6: 301-302.   DOI
39 Yan, X.L., Chen, Y.Y., Guan, B.C. and Fu, C.X. 2009. Eleven novel microsatellite markers developed from the living fossil Ginkgo biloba (Ginkgoaceae). Conservation Genetics 10: 1277-1279.   DOI