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Molecular Cloning of a cDNA Encoding Putative Calreticulin from the Silkworm, Bombyx mori  

Kim, Seong-Ryul (College of Natural Resources and Life Science, Dong-A University)
Lee, Kwang-Sik (College of Natural Resources and Life Science, Dong-A University)
Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
Kang, Seok-Woo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology)
Nho, Si-Kab (College of Agriculture and Life science, Kyungpook National University)
Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.6, no.1, 2003 , pp. 93-97 More about this Journal
Abstract
We describe here the cloning of a cDNA encoding putative calreticulin (CRT) from the silkworm, Bombyx mori. The CRT cDNA comprised of 1,194 bp encoding 398 amino acid residues. B. mori. CRT has a HDEL sequence at the end of the C-domain. The B. morl, CRT showed 88% protein sequence identity to the G. mellonella CRT, 71 % to A. aegypti CRT, and 63% to H. sapiens CRT, Phylogenetic analysis revealed that the deduced amino acid sequences of the B. mori CRT formed a highly inclusive subgroup with other insect CRTs. Northern blot analysis exhibited an expression of the B. mori CRT gene in the fat body, evidencing the fat body as a major site for CRT synthesis.
Keywords
Insect; Silkworm; Bombyx mori; Calreticulin; cDNA sequences; Phylogeny;
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