• 수산해양기술연구
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• 제52권4호
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• pp.318-324
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• 2016

Commercial split beam echosounder (ES70) installed on a krill fishing vessel was calibrated in order to utilize it in estimating biomass of Antarctic krill (Euphausia superba). The method of calibration was to analyze the difference between the bottom backscattering strength of the commercial split beam echosounder (i.e. ES70) and the scientific echosounder (i.e. EK60) at one of transects near South Shetland Islands designated by CCAMLR. 38 kHz and 120 kHz were used for the calibration, and krill swarm signal levels obtained from multi frequencies, was examined to verify the calibration result. The analysis result indicated possibility of calibration by bottom backscattering strength, since the proportion of krill swarm signals within 2 dB < $S_{V\;120\;kHz-38\;kHz}$ < 12 dB (i.e. a common $S_{V\;120\;kHz-38\;kHz}$ range of 38 kHz and 120 kHz to be an indicator of Antarctic krill) over the total acoustic signals were 26.95% and 92.04%, respectively before and after the calibration.

• 대한의생명과학회지
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• 제22권3호
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• pp.75-82
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• 2016

The immune system and neuroendocrine systems are the two key components that maintain bodily homeostasis. Peripheral blood specimens can indicate abnormalities in a body, which often cause various threats to human health, including devastating autoimmune or metabolic diseases. To develop a treatment regimen for such diseases, experimental animal models are indispensable to researchers in academic fields. In this study, we examined the peripheral blood of 3 representative mouse strains (ICR, Balb/c, and C57Bl/6), which are widely used, to investigate whether there is a difference in reference range according to animal model. We performed hematological and chemistry analysis on individuals of both genders. The results of hematology analysis showed that the number of most types of blood cells was lower in ICR than in the other two strains. The results of chemical analysis revealed no specific pattern, but different patterns according to the individual indicator. Although the distinction between ICR and B6 was prominent, differences between Balb/c and B6 were also observed for several indicators. For some indicators, totally different patterns existed between females and males. Conclusively, this study provides the information that 3 experimentally representative mouse models have their own basal levels of blood components, suggesting the importance of a careful choice of a proper mouse model in research into immune or metabolic diseases, to exclude any biases.

• 대한본초학회지
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• 제34권2호
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• pp.59-66
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• 2019

Objectives : Decrease in muscle mass and loss of muscle function due to aging are associated with various diseases. As interest in healthy aging increases, efforts to prevent and treat muscle hypoxia as an illness are increasing. Considering the physical limitations, a pharmacologic approach to the treatment of myopenia is needed. Methods : Terminalia chebula Rets has a wide range of pharmacological effects and is used as a medicinal product in traditional medicine. However, the drug effect on the treatment of muscle disorders has not been revealed. The purpose of this study was to evaluate the value of water extract of Terminalia chebula (WETC) as a therapeutic agent to relieve symptoms of muscle hypoxia. Results : WETC showed strong radical scavenging ability. In addition, WETC increased cell activity of myoblast, and we observed that WETC induces myoblast differentiation by immunoblot analysis using differentiation protein markers as well as cell morphology of myoblast. Based on these results, we examined the effect of chebulic acid, chebulagic acid, gallic acid, geraniin, and punicalagin on cell activity and differentiation of myoblasts. Gallic acid significantly increased cell activity of myoblast, and it was found to be an effective substance which not only induces myoblast differentiation but also promotes proliferation. Conclusions : We suggest that the WETC with antioxidant effect and its indicator gallic acid on cell activity, proliferation and differentiation of myoblast can be studied and developed as a food and medicine for prevention and treatment of various muscle diseases.

• Journal of Ecology and Environment
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• 제46권1호
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• pp.76-84
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• 2022

Background: Climate and livestock grazing are key agents in determining current Mongolian steppe vegetation communities. Together with plant coverage or biomass, palatability of steppe community is regarded as a useful indicator of grassland degradation, in particular, at grazing hotspots in arid and semi-arid grasslands. This study analyzed relationships between livestock grazing pressure and steppe vegetation palatability at three summer pastures with different aridity (dry, xeric, and mesic) and livestock numbers (1,100, 1,800, and 4,100 sheep units, respectively). At each site, it was surveyed coverage, biomass, and species composition of different palatability groups (i.e., palatable [P], impalatable [IP], and trampling-tolerant [TT]) along a 1-km transect from grazing hotspots (i.e., well) in every July from 2015 to 2018. Results: In results, total vegetation coverage increased with wetness, 7 times greater at mesic site than dry one in averages (33.1% vs. 4.5%); biomass was 3 times higher (47.1 g m^-2 vs. 15.7 g m^-2). Though P was the dominant palatability group, the importance of IP in total coverage increased with aridity from mesic (0.6%) to dry (40.2%) sites. Whereas, TT increased with livestock numbers across sites. Locally, IP was observed more frequently near the wells and its spatial range of occurrence becomes farther along the transects with aridity across sites from mesic (< 100 m) to dry (< 700 m from the well). Conclusions: Our results showed that the importance of IP and its spatial distribution are different at both local and regional scales, indicating that the palatability parameters are sensitive to discern balance between selective-grazing demand and climate-driven foraging supply in Mongolian rangelands.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1610-1610
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• 2001

Microbiological examination of silage is of little value in gauging the outcome of silage, and so chemical analysis is more reliable and meaningful indicator of quality. On the other hand chemical assessments of the principal fermentation products provide an unequivocal basis on which to judge quality. Livestock require energy, protein, minerals and vitamins from their food. While fresh forages provide these essential items, conserved forages on the other hand may be deficient in one or more of them. The aim of the conservation process is to preserve as many of the original nutrients as possible, particularly energy and protein components (Woolford, 1984). Silage fermentation is important to preservation of forage with respect of feeding value and animal performance. Chemical and bacteriological changes in the silo during the fermentation process can affect adversely nutrient yield and quality (Moe and Carr, 1984). Many of the important chemical components of silage must be assayed in fresh or by extraction of the fresh material, since drying either by heat or lyophilisation, volatilises components such as acids or nitrogenous components, or effects conversion to other compounds (Abrams et al., 1987). Maize silage dorms the basis of winter rations for the vast majority of dairy and beef cattle production in Uruguay. Since nutrient intake, particularly energy, from forages is influenced by both voluntary dry matter intake and digestibility; there is a need for a rapid technique for predicting these parameters in farm advisory systems. Near Infrared Reflectance Spectroscopy (NIRS) is increasingly used as a rapid, accurate method of evaluating chemical constituents in cereals and dried forages. For many years NIRS was applied to assess chemical composition in dry materials (Norris et al., 1976, Flinn et al., 1992; Murray, 1993, De Boever et al., 1996, De la Roza et al., 1998). The objectives of this study were (1) to determine the potential of NIRS to assess the chemical composition of dried maize samples and (2) to attempt calibrations on undried samples either for farm advisory systems or for animal nutrition research purposes in Uruguay. NIRS were used to assess the chemical composition of whole - plant maize silage samples (Zea mays, L). A representative population of samples (n = 350) covering a wide distribution in chemical characteristics were used. Samples were scanned at 2 nm intervals over the wavelength range 400-2500 nm in a NIRS 6500 (NIRSystems, Silver Spring, MD, USA) in reflectance mode. Cross validation was used to avoid overfitting of the equations. The optimum calibrations were selected on the basis of minimizing the standard error of cross validation (SECV). The calibration statistics were R$^2$ 0. 86 (SECV: 11.4), 0.90 (SECV: 5.7), 0.90 (SECV: 16.9) for dry matter (DM), crude protein (CP), acid detergent fiber (ADF) in g kg$\^$-1/ on dry matter, respectively for maize silage samples. This work demonstrates the potential of NIRS to analyse whole - maize silage in a wide range of chemical characteristics for both advisory farm and nutritive evaluation.

• 한국균학회지
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• 제41권2호
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• pp.57-66
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• 2013

치악산 지역의 7개 세부조사구에서 2002~2003년도에 수집 분류 동정된 버섯은 총 53속, 84종, 143개체이었다. 지역 간 중복 발생된 버섯은 총 43종이었으며 이중 2개 및 3개 구역에서 중복 발생된 버섯은 2002년 12개 및 3개종, 2003년 7개 및 4개종이었다. 상대 종 밀도지수(RSD)는 7개 구간에서 0.179~0.226 범위 이었으며 종 다양성 지표 중 유사도(Similarity index: C)는 지역(7개 조사구)간 11.3%~15.9% 범위이었고 평균 13.1% 이었다. 조사지역 전체의 버섯발생은 같은 시기에 인근 지역에 중복 발생할 가능성이 있는 지역 간 유사도보다 다음연도에 동일 지역에서 다시 발생할 가능성인 년차간 유사도가 8.2% 더 높았다. 전체 조사기간의 버섯 종 다양성과 관련된 6가지 지표 중 지역 간 변이계수(CV)는 개체수(10.5%)>우점도 (9.2%)>다양도 (8.9%)>종수 (8.5%)>풍부도(7.4%)>균등도(2.2%) 순 이었다. 버섯 종 다양성 지표 중 풍부도지수(Richness index: R1)는 지역 간 4.85~6.01 범위의 평균 5.36 및 지역전체 16.72, 다양도 지수(Variety index: V1)는 지역 간 14.44~18.66 범위의 평균 16.24 및 지역 전체 68.82, 균등도 지수(Evenness index: E2)는 지역 간 0.926~0.982범위의 평균 0.95 및 지역전체 0.819, 우점도 지수(Dominance index: D1)는 지역 간 0.055~0.073 범위의 평균 0.071 및 지역전체 0.018 이었다. 개체수는 종 다양성 지수들과 유의성 있는 상관관계를 보여주지 않았으나, 종수는 풍부도, 다양도 및 우점도 지수와 유의성 있는 상관관계가 있었다. 종 다양성 지수 간 상호 유의성 있는 상관관계를 보여주는 지수들은 풍부도와 다양도 및 우점도이었다.

• 한국정보통신학회논문지
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• 제20권4호
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• pp.819-825
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• 2016

본 논문에서는 기존의 위상 고정 루프를 병렬 형태로 이중 루프를 구성하였다. 두 개의 루프를 통해서 전달 특성에 따라 원하는 크기의 대역폭을 만든다. 대역 폭의 형태는 동작하는 주파수 대역에서 잡음을 최소화 할 수 있는 위상 고정 루프를 설계하였다. 제안한 위상고정루프는 두 가지 필터를 제어하기 위하여 두 개의 기울기 값을 가지는 전압제어 발진기를 사용하였다. 또한 정확한 위상 고정을 위하여 위상 고정 상태 표시기를 사용하였다. 전체적인 위상 고정 루프가 안정적인 동작하기 위하여 각 각의 루프가 각각 $58.2^{\circ}$, $49.4^{\circ}$의 위상 여유를 가지고 있으며 두 개의 루프를 합쳤을 때에도 $45^{\circ}$이상의 안정적인 위상 여유를 가지는 것을 확인 할 수 있다. 제안된 위상 고정 루프는 1.8V 0.18um CMOS 공정을 이용하여 설계 되었다. 시뮬레이션 결과는 이중 루프를 가지고 위상고정루프의 구조가 원하는 출력 주파수를 생성하며 안정적으로 동작하는 것을 보여 주었다.

• 제어로봇시스템학회논문지
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• 제21권9호
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• pp.898-902
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• 2015

Alternative radio-navigation technologies aim at providing continuous navigation solution even if one cannot use GNSS (Global Navigation Satellite System). In shadowing region such as indoor environment, GNSS signal is no longer available and the alternative navigation system should be used together with GNSS to provide seamless positioning. For soldiers in battlefield where GNSS signal is jammed or in street battle, the alternative navigation system should work without positioning infrastructure. Moreover, the radio-navigation system should have scalability as well as high accuracy performance. This paper presents a TWR (Two-Way-Ranging)-based cooperative positioning system (CPS) that does not require location infrastructure. It is assumed that some members of CPS can obtain GNSS-based position and they are called mobile anchors. Other members unable to receive GNSS signal compute their position using TWR measurements with mobile anchors and neighboring members. Error propagation in CPS is analytically studied in this paper. Error budget for TWR measurements is modeled first. Next, location error propagation in CPS is derived in terms of range errors. To represent the location error propagation in the CPS, Location Error Propagation Indicator (LEPI) is proposed in this paper. Simulation results show that location error of tags in CPS is mainly influenced by the number of hops from anchors to the tag to be positioned as well as the network geometry of CPS.

• Asian-Australasian Journal of Animal Sciences
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• 제15권2호
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• pp.274-278
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• 2002

A culture system for lactating rat mammary acini was evaluated, where the primary indicator of performance was lactose secretion, measured by a sensitive bioluminescence assay. Lactose secretion was reduced by half (p<0.01) over the first 6 h of culture by overnight feed withdrawal (FW) from tissue donors but was sensitive to increased glucose concentration in the culture media (p<0.001) up to 30 mM. Lactose production of cells from fed donors over the first 6 h in culture in 30 mM glucose was 8.9 fmol/cell/h - a rate calculated to be about half that in vivo. No significant difference was shown in lactose secretion by cells from fed or FW rats over 6-24 h. Lactose secretion was 3.6 fmol/cell/h by cells from fed animals in 40 mM glucose concentration media over the 6-24 h culture period. Addition of insulin to the culture media had no effect on rates of lactose secretion while addition of prolactin and hydrocortisone, with or without insulin, significantly (p<0.001) decreased lactose production over both 0-6 h and 6-24 h culture periods. Lactose synthesis in vitro was significantly enhanced by aeration of the media during collagenase digestion of mammary tissue (p<0.05). No improvement in lactose secretion was effected by shaking of cells during culture, Matrigel coating of culture dishes or change in cell density over a range up to 2.5 million cells per ml.

• Journal of Preventive Medicine and Public Health
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• 제41권3호
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• pp.195-199
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• 2008

Objectives : Malondialdehyde (MDA), a lipid peroxidation by-product, has been widely used as an indicator of oxidative stress. Urinary 2-naphthol, a urinary PAH metabolite, is used as a marker of ambient particulate exposure and is associated with lung cancer and chronic obstructive pulmonary disease. However, the stability and intra-individual variation associated with urinary MDA and 2-naphthol have not been thoroughly addressed. The objective of this study was to assess the stability and intra-individual variation associated with urinary MDA and 2-naphthol. Methods : Urine samples were collected from 10 healthy volunteers (mean age 34, range $27{\sim}42$ years old). Each sample was divided into three aliquots and stored under three different conditions. The levels of urinary MDA and 2-naphthol were analyzed 1) just after sampling, 2) after storage at room temperature ($21^{\circ}C$) for 16 hours, and 3) after storage in a $-20^{\circ}C$ freezer for 16 hours. In addition, an epidemiological study was conducted in 44 Chinese subjects over a period of 3 weeks. The urinary MDA and 2-naphthol were measured by HPLC three times. Results : There was no difference in the levels of urinary MDA and 2-naphthol between the triplicate measurements (n=10, p=0.84 and p=0.83, respectively). The intra-class correlation coefficients (ICC) for urinary MDA and 2-naphthol were 0.74 and 0.42, respectively. However, the levels of PM2.5 in the air were well correlated with the levels of both MDA and 2-naphthol in the epidemiological study. Conclusions : These results suggest that urinary MDA and 2-naphthol remain stable under variable storage conditions, even at room temperature for 16 hours, and indicate that these markers can be used in epidemiological studies involving various sample storage conditions. The intra-CC of urinary 2-naphthol and MDA were acceptable for application to epidemiological studies.