• Journal of Plant Biotechnology
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• v.35 no.4
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• pp.275-280
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• 2008

One of the limitation for Agrobacterium-mediated transformation via organogenesis from cotyledon explants routinely in cucumber is the production of chimeric plants. To overcome the limitation, Agrobacterium-mediated transformation system via somatic embryogenesis from hypocotyl explants of cucumber (c.v., Eunsung) on the selection medium with paromomycin as antibiotics was developed. The hypocotyl explants were inoculated with Agrobacterium tumefaciens strain EHA101 carrying binary vector pPTN290; then were subsequently cultured on the following media: co-cultivation medium for 2 days, selection medium for $5{\times}14$ days, and regeneration medium. The T-DNA of the vector (pPTN290) carried two cassettes, Ubi promoter-gus gene as reporter and 35S promoter-nptll gene conferring resistance to paromomycin as selectable agent. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to paromomycin indicated by the growth of putative transgenic calli on selection medium amended with 100mg/L paromomycin, and GUS gene expression. Forty eight clones (5.2%) with GUS gene expressed of 56 callus clones with resistance to paromomycin were independently obtained from 928 explants inoculated. Of 48 clones, transgenic plants were only regenerated from 5 clones (0.5%) at low frequency. The histochemical GUS assay in the transgenic seeds ($T_1$) also revealed that the gus gene was successfully integrated and segregated into each genome of transgenic cucumber.

• The Journal of Korean Physical Therapy
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• v.31 no.5
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• pp.328-332
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• 2019

Purpose: This study examined the effects of transcranial direct current stimulation (tDCS) on the static balance ability of patients with back pain. Methods: The subjects comprised of 30 males and females in their 20s, who were divided into two groups with and without tDCS. The balance posture ratio score was calculated to determine the changes in balance ability before and after the application of tDCS using balance equipment. A paired t-test was conducted to determine the changes over time, and an independent t-test was performed to determine the changes between the groups. The significance level was set to ${\alpha}=0.05$. Results: A significant difference in the changes in the static balance ability of CTDCSG between before and after applying tDCS was observed while the subjects' eyes were open (p<0.05). A comparison between the groups after the experiments revealed significantly increased changes in CTDCSG compared to STDCSE (p<0.05). The changes in static balance ability were not significant when the subjects' eyes were closed (p>0.05). Conclusion: The application of tDCS positively changed the static balance ability of patients with back pain. The results of this study showed that tDCS could be used as an intervention to increase the static balance ability of patients with back pain in the clinical field. The study results could be used further as foundational data for future studies on tDCS.

• Journal of Convergence for Information Technology
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• v.9 no.3
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• pp.104-111
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• 2019

The purpose of this study is to know the change of brain wave with stress by SUKI pressure alternative therapy. The experiment group was 12 students(male 6/female 6) with complained upper trapezius pain. Calculated the brain wave before and after stimulate the upper trapezius trigger point by SUKI and the stimulate time was 3min each persons(FP2, F3, F4, FP1, T3, T4, P3, P4). the experimet periods was 5times in a weeks with EEG(QEEG-S). The Date collecting used by Telescan(LXE5209). All the data was analyzed with SPSS 22.0 for window program. To compare the differences before and after the point pressure method, a corresponding sample of t-test was performed, and the statistical significance level was p<.05. The results was followed. The points of Fp2, F3, F4(*p<0.049, *p<0.042, *p<0.019) of EEG was showed a significant differences but Fp1, T3, T4, P3, P4 points did not showed. The SUKI alternative medicine techniques had a reduced effects for the some kind of brain stress. It is need to continuous research in the future.

• Journal of the Korean Society for Marine Environment & Energy
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• v.3 no.3
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• pp.41-49
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• 2000

When sewage and industrial wastewater are treated to improve the coastal water quality, we usually consider the reduction of BOD and/or COD. Due to the inflow of untreated nitrogen and phosphorus, however, the coastal water has eutrophicated. Thus, to improve the water qualify, it is necessary to treat and reduce nitrogen and phosphorus which are the factors limiting algal growth. Hence, with regard to the adaption of Ocean Outfall for the treatment of wastewater in Suyoung Bay, we studied the effectiveness and environmental assessment of Ocean Outfall of which cost is lower than that of advanced treatment, The effectiveness of Ocean Outfall of the second treatment effluent in Nambu S.T.P. was simulated, using the eco-hydrodynamic model as far field model the result showed that the water quality of coastal area receiving wastewater on the surface sea has improved. But the concentrations of nitrogen and phosphorus around the diffuser of Ocean Outfall system can cause many problems on estuary, such as red tide, eutrophication and aquatic toxicity.

• Journal of Digital Convergence
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• v.18 no.5
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• pp.297-304
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• 2020

This study was conducted to identify the factors influencing nursing professionalism of nursing students and to prepare measures to enhance them. This study is a descriptive survey of first-year nursing students at one university, which collected data through structured questionnaires from October 1 to October 31, 2019, and there were 203 analyzed with SPSS 22.0. The data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation, and multiple regression. According to the results, the most significant factors affecting nursing professionalism included academic self-efficacy (β =.51, p<.001), followed by psychological well-being (β=.31 p<.001), and self-leadership(β=.14, p=.043). These variables explained 23.8% of the total variance in nursing professionalism. Based on the results of this study, it is expected to be used as basic data for various curriculums and programs to improve academic self-efficacy, strengthen psychological well-being, and cultivate self-leadership, thereby improving nursing professionalism of nursing students.

• Journal of Digital Convergence
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• v.20 no.4
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• pp.127-132
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• 2022

This study is a descriptive research to understand the leadership, communication ability, peer care behavior and relationship of nursing students. Data were collected from 192 nursing students in November 2021. The data were analyzed by t-test, ANOVA, Pearson's correlation ciefficients, using SPSS 28.0. As a result of the study, revealed positive correlations between leaderships and communication competence(r=.634, p<.001), communication competence and peer group caring interaction(r=.390, p<.001), leaderships and peer group caring interaction(r=.285, p<.001). Therefore, it is necessary to develop educational environment and programs to improve leadership, communication ability, and peer care behavior of nursing college students in the future.

• Journal of Digital Convergence
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• v.15 no.1
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• pp.311-320
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• 2017

The purpose of this study investigates the relationships between nurses' image, professional self-concept and nursing professionalism in nursing students, and explore the plan of nursing education for the establishment of exemplary nursing professionalism in nursing students. A convenience sample of 292 subjects were recruited from one university in G city. And data were collected from November 11 to November 15, 2015. Data were analyzed using t-test, ANOVA, Pearson's Correlation, Stepwise Multiple linear regression analyses with SPSS 21.0 Program. The nursing professionalism of nursing students showed a significantly positive correlation of nurses' image(r=.821, p<.001) professional self-concept(r=.701, p<.001). Fifty-seven percentage of the variance was explained by nurses' image, professional self-concept and grade. Therefore, on the basis of this study's result, when nursing educational programs are developed to make positive professionalism, these factors need to be consider.

• Research in Community and Public Health Nursing
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• v.21 no.2
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• pp.252-262
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• 2010

Purpose: Designed to examine the effects of aerobic exercise therapy on elders' physical functions. Methods: Selected from an elderly welfare center in an agricultural district located in N City. Thirty seven elders were selected in the experimental group and 38 in the control group, and all the subjects aged over 65. Collected data were statistically analyzed by SPSS/PC 12.0 Win. Detailed data analysis methods were Chi-square, Fisher's exact test, Kolmogorov-Smirnov test, t-test, Mann-Whitney u-test, paired t-test, and Wilcoxon's rank sum test. Results: The first hypothesis "The experimental group who had the aerobic exercise therapy will have greater development in lower leg muscular strength compared to the control group" was supported (t=8.95, p<.001). The second hypothesis "Aerobic exercise therapy participants will show greater development in lower leg endurance" was supported (t=6.12, p<.001). The third hypothesis "Aerobic exercise therapy participants will show greater development in flexibility" was supported (U=49.00, p<.001). The forth hypothesis "Aerobic exercise therapy participants will show greater development in balance" was supported (U=322.00, p<.001). Conclusion: The aerobic exercise therapy showed positive effects on physical functions of the elderly in a rural area.

• Korean Journal of Occupational Health Nursing
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• v.29 no.4
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• pp.333-341
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• 2020

Purpose: This study aimed to determine the mediating effect of emotional intelligence on the relationship between the social support and job satisfaction of general hospital nurses. Methods: The data of 197 nurses in general hospitals located in three different cities were collected for analysis. Collected data were analyzed using the t-test, ANOVA, Scheffé test, Pearson's correlation coefficients, and hierarchical multiple regression with SPSS/WIN 23.0. Results: The social support of the subjects showed positive correlations with job satisfaction (r=.56, p<.001) and emotional intelligence (r=.42, p<.001). Emotional intelligence and job satisfaction were positively correlated (r=.54, p<.001). Emotional intelligence was found to perform the partial mediation in the relationship between social support and job satisfaction. Conclusion: To improve job satisfaction, an organizational culture which enhances social support may be required as an intervention strategy, and programs should be developed to induce positive emotional intelligence.

• Journal of the Korean Applied Science and Technology
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• v.35 no.3
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• pp.975-984
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• 2018

The purpose of that was to investigate the potential of P. Herba extracts as phytonutrient active ingredients. In order to elucidate the P.Herba ethanol extracts were examined DPPH radical scavenging activity, NO production, protective effects against oxidative stress in HaCaT cells, anti-inflammatory activity, antimicrobial activity, anti-allergic effects, and inhibition of ${\beta}$-hexosaminidase expression. The antioxidative activity of the P. Herba extracts was compared, and the antioxidative activity of the ethanol extract was found to be superior. No significant cytotoxicity was observed in HaCaT, RAW 264.7, and RBL-2H3 cells. The protective effect of the extracts against oxidative stress induced by hydrogen peroxide ($H_2O_2$) was examined in HaCaT cells, and it was found to be 83% This concentration refers to which extract ethanol at $100{\mu}g/mL$. The anti-inflammatory activity of the extracts was examined in RAW 264.7 cells, and NO production was suppressed even at low concentrations. In addition, the concentration-dependent antimicrobial activities of the extracts were demonstrated in several bacterial strains, such as those of S.aureus, S.epidermidis and P. acnes. Based on the findings from this study, Portulacae Herba extracts could be used as physiological active substance that possess antioxidative, anti-inflammatory, and antimicrobial properties.