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Studies on the bionomics of Oriental moth, Cnidocampa flavescens WALKER, damaging to the persimmon tree in the southern part of Korea (감나무에 기생하는 Cnidocampa flavescens WALKER의 생태에 관한 연구)

  • Chung S. T.;Lee E. S.
    • Korean journal of applied entomology
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    • v.4
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    • pp.39-46
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    • 1965
  • The author studied on the bionomics of Oriental moth. Cnidocampa flavescens WALKER, damaging to the persimmon tree n the southern part of Korea from 1964 to 1965. The results can be summarized as follows; 1. Emergence peak period of Oriental moth was mid-June in Taegu district and eggs are deposited on the opposite side of persimmon tree leaf. Specially most of eggs are deposited on the terminal part of opposite side and peak period s also mid-June. 2. Hatched Percentage of eggs was $84.4\%$ in 1964 while $96.1\%$ in 1965 at the rearing room. Mean egg Period was $5.984\pm0.162$ in 1964 while $6.262\pm0.094$ days in 1965. Thus during two years, the egg period was about 6 days. 3. In the growth ratio of Oriental moth fed on various host plants persimmon tree, Acer negund, Hazel-wood and Platanus, the best growth ratio was shown on the leaf of Hazel-wood from 1st till 3rd instar, but, on the contrary, persimmon tree was the best from 4th till the last instar. The growth ratio of head width was also the same tendency as the body length above mentioned. Individuals fed on the leaf of platanus were dead after 20 days. 4. Oriental moth has one generation a year and molts 6 times. The first molting occurred in 5 hours after hatched, and the other moltings were done at f days intervals. After 3 days since the last molting, larvae made the non for over-winter in it. 5. As the bristles on the process of larval body are different from each position and instar, judgement of instars are possible by the counting of bristles on the body according to the Table 8. Specially the bristle of L. 2., D. 2, 3 ,8. 10. and L. 1, 3, 4, 5, 6, 7, are perfectly different from each instar. From these bristles, instars can be recognized easily. 6. Pupation of larvae in the over-wintered cocoon on the stem of persimmon tree was done in mid-May and continued will early June when emergence will take place. 7. Mean number of eggs in the ovary was $1325.5\pm2.7182$

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Analysis of Genetic Characteristics and Probability of Individual Discrimination in Korean Indigenous Chicken Brands by Microsatellite Marker (MS 마커를 이용한 토종닭 브랜드의 유전적 특성 및 개체 식별력 분석)

  • Suh, Sangwon;Cho, Chang-Yeon;Kim, Jae-Hwan;Choi, Seong-Bok;Kim, Young-Sin;Kim, Hyun;Seong, Hwan-Hoo;Lim, Hyun-Tae;Cho, Jae-Hyeon;Ko, Yeoung-Gyu
    • Journal of Animal Science and Technology
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    • v.55 no.3
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    • pp.185-194
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    • 2013
  • Microsatellite markers have been a useful genetic tool in determining diversity, relationships and individual discrimination studies of livestock. The level of genetic diversity, relationships among two Korean indigenous chicken brand populations (Woorimatdag: WR, Hanhyup3: HH) as well as two pure populations (White Leghorn: WL, Rhode Island Red: RIR) were analyzed, based on 26 MS markers. A total of 191 distinct alleles were observed across the four chicken populations, and 47 (24.6%) of these alleles were unique to only one population. The mean $H_{Exp}$ and PIC were estimated as 0.667 and 0.630. Nei's $D_A$ genetic distance and factorial correspondence analysis (FCA) showed that the four populations represented four distinct groups. However, the genetic distance between each Korean indigenous chicken brand (WR, HH) and the pure population (WL, RIR) were threefold that among the WR and HH. For the STRUCTURE analyses, the most appropriate number of clusters for modeling the data was determined to be three. The expected probabilities of identity among genotypes of random individuals (PI) were calculated as $1.17{\times}10^{-49}$ (All 26 markers) and $1.14{\times}10^{-15}$, $7.33{\times}10^{-20}$ (9, 12 with the highest PI value, respectively). The results indicated that the brand chicken breed traceability system employing the own highest PI value 9 to 12 markers, and might be applicable to individual identification of Korean indigenous chicken brand.

Biology of Three Species of the Genus Tipula (Diptera: Tipulidae) in Korea (한국산 Tipula속(파리목 : 각다귀과) 3종의 생태)

  • Kim, Dong-Sang;Lee, Jong-Eun
    • Korean journal of applied entomology
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    • v.46 no.2
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    • pp.201-212
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    • 2007
  • This study was conducted to investigate the biology of three species, Tipula latemarginata Alexander, T. nova Walker and T. aino Alexander, of the Genus Tipula in Korea. Field surveys for the biology of the species were carried out at the three sites of Neaseong Stream area in Bongwha County from January 2001 to December 2005. Also many individuals of the species were reared in laboratory to investigate the biology. The larval habitats of the species were streams, rivers, paddy fields, damp earth or leaky revetments. The Tipula larvae were herbivorous. Newly-hatched first instar larvae fed on soft algae, and the larvae gradually fed on leaves, stems and roots of a variety of plants or hard leaf litters, as they grew. During the molting of the larvae their body kept moving from left to right and up and down. Molting usually took not more than 2 hours, and about four hours after molting, the larvae started to eat heavily. The Tipula larvae in aquatic places moved to drier land for pupation and went through short prepupal stage lasting 1-2 days for pupation at fourth instar larval stage. When emerging, the Tipula pupae placed their head and thorax on the earth, but the other parts in the earth. Emergence from the pupal case required about 20 min. to one hour. Mating of Tipula adults took place within 5-7min. after emergence and the duration of mating was about 40 min. The female adults of the species laid eggs by walking with 3 pairs of legs over the damp earth or algal beds. Their body was positioned vertically on the ground with their wings spread $120^{\circ}$ and legs landed on the surface. The oviposition usually took place from one day to 4 days after emergence and the number of eggs carried by female adults were an average of 501-760 per individual. Tipula adults didn't normally feed, and drank water only occasionally. For a few days after emergence, the adults reared in the laboratory rarely drank water. As they neared to death, however, they frequently drank water. The longevity of adults reared in the laboratory with only water during the summer was ca. 4-9 days and males usually survived a little longer than females. The longevity of T. nova was increased 3 times or more as much by feeding them 3% sugar water. Male adults of T. latemarginata outnumbered female adults by 2.6 : 1 in the fields.

Investigation of Microsatellite Markers for Traceability and Individual Discrimination of Korean Native Ducks (한국 토종오리의 개체 식별 및 품종 구분을 위한 Microsatellite 마커 탐색)

  • Seo, Dong Won;Sultana, Hasina;Choi, Nu Ri;Kim, Yeon Su;Jin, Shil;Heo, Kang Nyeong;Jin, Seon Deok;Lee, Jun Heon
    • Korean Journal of Poultry Science
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    • v.42 no.1
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    • pp.1-8
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    • 2015
  • Recently, duck meat consumption has been rapidly increased because consumers recognized duck meat for healthy food. In relation to this, Korean duck industry need to develop Korean native duck (KND) breed for both conservation perspective and self-sufficient of the breeding stocks. In this study, 24 microsatellite (MS) markers were investigated for classification of KND and commercial duck (CD) breeds in the Korean market. Using these MS markers, the calculated number of alleles (K), expected heterozygosity (He) values and polymorphic information contents (PIC) were 1~16, 0~0.865 and 0~0.841, respectively. Also, the expected probability of identical values in random individuals (PI), random sib ($PI_{sib}$) and random half-sib ($PI_{half-sib}$) were estimated as $1.64{\times}10^{-16}$, $2.60{\times}10^{-7}$ and $1.30{\times}10^{-12}$, respectively. The results indicated that the expected probabilities of identity powers were enough for the individual identification. However, KND and CD breeds were not fully discriminated well using the 24 MS markers, which may CD and KND has shared same origin or crossbred. Therefore, further studies will be ultimately needed for developing a genetically pure line of KND breed even though the DNA markers used. Finally, these results will provide useful information for individual traceability system in ducks.

A Study in an Effective Programs for Emergency Care Delivery System (응급의료 전달체계의 충실 방안)

  • Kwon Sook Hee
    • Journal of Korean Public Health Nursing
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    • v.9 no.1
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    • pp.83-102
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    • 1995
  • As the society is being industrialized, the fast-paced economic development that has caused substantial increase in cerebrovascular and coronary artery diseases and the industrial development and increased use of means of transportation have resulted in the rapid rise of incidents in external injuries as well. So the pubic has become acutely aware of the need for fast and effective emergency care delivery system. The goal of emergency care delivery system is to meet the emergency care needs of patients. The emergency care delivery system is seeking to efficiently satisfy the care needs of people. Therefore the purpose of this study is designed to develop an effective programs for emergency care delivery system in Korea. The following specific objectives were investigated. This emergency care delivery system must have the necessary man power, for transfering the patients, communication net work, and emergency care facilities. 1) Man power Emergency care requires n0t only specialized traning in the emergency treatment but also knowledge and experience i11 other related area, so emergency care personnel traning program should be designed in order to adapt to the specific need of emergency patients. It will be necessary to ensure professional personnel who aquires the sufficient traning and experience for emergency care and to look for legal basis. We have to develop re-educational programs for emergency nurse specialist. They should be received speciality of emergency nursing care so that they will work actively and positively in emergency part. Emergency medical doctor and nurse specialist should be given an education which is related in emergency and critical care. Emergency care personnel will continue to provide both acute and continuing care as partner with other medical team. 2) Transfering the patients. Successful management of pre-hospital care requires adequate traning for the emergency medical technician. Traning program should be required to participate in a actual first aids activites in order to have apportunities to acquire practical skills as well as theoretical knowledge. The system of emergency medical technician should be remarkablly successful with first responder firefighters. Establishing this system must add necessary ambulances operating at any given time. It will be necessary to standardize the ambulance size and equipment. Ambulance should be arranged with each and every fire station. 3) Communication net work. The head office of emergency commumication network should be arranged with the head office of fire station in community. It is proposed that Hot-line system for emergency care should be introduce. High controlled ambulance and thirtial emergency center should simultaneously equip critical-line in order to communication with each other. Ordinary ambulance and secondary emergency facility should also simultaneously equip emergency-line in order to communication with each other. 4) Emergency care facilities. Primary emergency care facilities should be covered with the ambulatory emergency patients-minor illness and injuires. Secondary emergency care facilities should be covered with the emergency admission patients. Third emergency care center should be covered with the critical patients who need special treatments and operation. Secondary and third emergency care facilities should employ emergency medical doctor and emergency nurse specialist to treat in-patients with severe and acute illness and multiple injuires. It should be fashioned for a system of emergency facilities that meets emergency patients needs. Provide incentives for increased number of emergency care facilities with traning in personal/clinical emergency care. 5) Finance It is recommended to put the finance of a emergency care on a firm basis. The emergency care delivery system should be managed by the government or accreditted organizations. In order to facilitate this relevant program the fund is needed for more efficient and effective emergency researchs, service, programs, and policy. 6) Gaining understanding and co-operation of pubic It is also important to undertake pubic education to improve understanding of first aids and C. P. R of individuals, communities and business. It is proposed that teachers and health officers be certified in C. P. R. The C. P. R education can be powerful influence save lives. Lastly appropriate emergency care information must be provided to the pubic for assisting them in choosing emergency care.

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Distribution and Seasonal Occurrence of Japanese Gall-forming Thrips, Ponticulothrips diospyrosi, in Korea (감관총채벌레(Ponticulothrips diospyrosi)의 분포와 발생소장)

  • Huh Wan;Huh Hye-Soon;Yun Ji-Eun;Son Jun-Ki;Jang Hyeon-Kyu;Uhm Ki-Baik;Park Chung-Gyoo
    • Korean journal of applied entomology
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    • v.45 no.1 s.142
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    • pp.59-66
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    • 2006
  • The damaged area by and the distribution of Japanese gall forming thrips, Ponticulothrips diospyrosi Haga et Okajima (Thysanoptera: Phlaeothripidae) in Korea were surveyed based on the answers of persimmon growers to questionnaire. Seasonal occurrence of each developmental stage was estimated by monitoring the individuals in damaged leaves, and effect of trap site and side of sticky board were studied by yellow sticky traps at three sweet persimmon orchards in Gimhae and Jinju (orchards A and B), Gyeongnam province, Korea in 2005. P. diospyrosi gave damage to astringent persimmon as well as sweet one. The damaged area of persimmon orchard has sharply increased since 2000, reaching 446 ha nationwide; Gyeongnam province was most severely damaged, followed by Gyeongbuk and Jeonnam provinces. Most of the farmers answered that they sprayed insecticides and removed the damaged leaves to control P. diospyrosi. The overwintered adults were observed in the damaged-rolled leaves from mid May and the 1st generation adults from early-mid June. No thrips were observed in the damaged leaves after mid July. Yellow sticky trap data showed that peak adults catch of the overwintered generation was mid May, and that of the 1st generation was mid-late June in Gimhae and late June in Jinju. Sticky traps set at orchard-pine forest border caught significantly more number of the 1st generation adults than those at the orchard center, even though timing of peak catches was not different between the two sites. There was a tendency of more catches on the side of sticky trap facing pine forest than the other side facing orchard center, even though the catches on both sides showed no statistical difference.

Studies on Genetic Diversity and Phylogenetic relationships of Chikso (Korea Native Brindle Cattle) Using the Microsatellite Marker (Microsatellite marker를 활용한 칡소의 유전적 다양성 및 유연관계 분석)

  • Choy, Yun Ho;Seo, Joo Hee;Park, Byungho;Lee, Seung Soo;Choi, Jae Won;Jung, Kyoung-sub;Kong, Hong Sik
    • Journal of Life Science
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    • v.25 no.6
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    • pp.624-630
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    • 2015
  • This study examined the genetic distance among Chikso (Korea native brindle cattle) in nine regional areas using allele frequencies and a genetic diversity analysis with microsatellite markers. The analysis of the genetic diversity and genetic relationships of 2068 Chikso (383 KW, 180 GG, 52 KN, 129 KB, 332 UL, 24 JN, 198 JB, 148 CN, 622 CB) was carried out using 11 microsatellite markers. The number of alleles, observed heterozygostiy (Hobs), expected heterozygosity (Hexp), and polymorphism information content (PIC) of the 11 microsatellite markers were 8–24, 0.672–0.834, 0.687–0.886, and 0.638–0.876, respectively. The expected probability of identity values in random individuals (PI), random half-sib (PIhalf-sibs), and random sibs (PIsibs) were estimated to be 5.24×10−19, 2.63×10−06, and 2.63× 10−06, respectively, indicating that these markers can be used for traceability systems in Chikso cattle. The results of a phylogenetic tree (neighbor-joining tree), principle component analysis (PCA), and factorial component analysis (FCA) revealed genetic distance among nine Chikso populations. In conclusion, this study provides useful basic data that can be utilized in Chikso breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing diversity of Chikso.

Genetic Monomorphism of the Natural Ilex cornuta Community at the Northern Range Limit in Buan, Jeollanam-do in Korea Revealed by AFLP Markers (AFLP 표지자에서 나타난 전라남도 부안의 북방한계지에 자생하는 호랑가시나무 군락의 유전적 단형성)

  • Hong, Kyung Nak;Park, Yu Jin;Lee, Jei Wan;Kim, Young Mi
    • Journal of Korean Society of Forest Science
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    • v.104 no.2
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    • pp.187-192
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    • 2015
  • Individuals in the population under a particular environmental condition influencing recurrently for a long time could locally adapted and local adaptation is of a fundamental importance in a breeding program, conservation activities of genetic resources or evolutionary biology. Plants at northern range limits have higher probability of expressing an adaptative genetic trait. The natural community at the northern range limit of Ilex cornuta (Chinese holly) in Buan, Jeollanam-do in Korea was composed of adults of 744 and seedlings of 211 (hereafter Community) and is designated as the Korean Natural Monument (No. 122) by the law. At two adjacent areas to Community, 85 (hereafter Plantation I) and 27 hollies (hereafter Plantation II) were planted respectively for preparations of the next generation. Eighty-five trees were sampled for genetic analysis in the three groups. Fifty-two (36%) of the total 143 amplicons were polymorphic from four AFLP primer combinations. A total of thirteen genotypes was identified and just one genotype was for 52 trees of Community. Seven and five genotypes were shown for Plantation I and II, respectively. There was no identical genotype between Community and Plantation (I or II) or between two plantation groups. Number of private loci was 2 for Community, 6 for Plantation I and 4 for Plantation II. We presumed their genetic backgrounds were quite different with one another and the plantation groups were made independently because they were different not only the genetic compositions but also their ages. Considering the genetic monomorphism by AFLP markers, observations of only male trees and asexual propagation as layerage or cuttage, the hollies in Community might be a genet by root suckering from a single male tree, not the results of selective removal of female trees for ornamental use in the past.

Seasonal Prevalence of Mosquitoes Collected with Light Trap - At a Pig Shed in the Vicinity of Daegu City, Korea- (誘蚊燈에 捕獲된 모기類의 季節的 發生消長: 大邱市隣近 한 豚舍를 對象으로)

  • 孫錫洛
    • The Korean Journal of Zoology
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    • v.27 no.2
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    • pp.117-125
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    • 1984
  • Mosquitoes were collected with light trap at a pig shed in the vicinity of Daegu city from mid-April to the end of November in 1981 and 1982. A total of 12,942 and 118,061 individuals were collected in 1981 and 1982 respectively. The collection comprised 77% females and 23% males in 1981, and 96% females and 4% males in 1982. The catches were classified into following 7 species: Culex (Culex) pipiens pallens, C. (C.) tritaeniorhynchus summorosus, Anopheles (Anopheles) sinensis, C. (C.) vagans, C. (C.) oritntelis, C. (C.) bitaeniorhynchus, Aedes (Aedimorphus) vexans nipponii. The former three species showed distinct seasonal prevalence. Arranged in the descending order in size of the catches, in 1981, Culex (Culex) pipiens pallens was 44.9% of the total collection (at sex ratio of 0.85), Anopheles (Anopheles) sinensis 42.9% (0.05), Culex (Culex) tritaeniorhynchus summorosus 12.1% (0.00). On the contrary, Culex (Culex) tritaeniorhynchus summorosus 70.4% (0.00), Anopheles (Anopheles) sinensis 25.2% (0.05), Culex (Culex) pipiens pallens 4.4% (2.19) in 1982. The monthly percentages of collected mosquitoes to the total collection were 0.1% (in 1981) and 0.0% (in 1982) in May; 3.5%, 1.3% in June; 50.0%, 33.9% in July; 37.1%, in August; 8.8%, 11.9% in September; 0.5%, 0.8% in October and 0.0% in November. As for seasonal prevalence, mosquitoes appeared in May in both years and began to increase in number from the first week of June in 1981, but from the second week of May, ahead of three weeks in 1982. The highest peak time in 1982 was the second week of August, two weeks later than the fifth week of July in 1981. Culex (Culex) pipiens pallens showed the maximum activity for the fifth week of July in 1981, but for the third week of July, ahead of two weeks in 1982. Culex (Culex) tritaeniorhynchus reached the highest peak for the second week of August in both years. Anopheles (Anopheles) sinensis showed the maximum activity for the fifth week of July in 1981, but for the third week of July, two weeks earlier in 1982. The highest peak times of three main species were compared respectively as folows. Culex (Culex) pipiens pallens had the highest peak time in common with Anopheles (Anopheles) sinensis, Culex (Culex) tritaeniorhynchus summorosus showed the maximum activity for the second week of August in 1982.

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Analysis of Growth Environment on Old Tree, a Natural Monument in Gyeongsangbuk-do (경북지역의 천연기념물 노거수 생육현황 분석)

  • Kim, Tae Sik;Cho, Sun Hee;Kang, Hee Jin
    • Korean Journal of Heritage: History & Science
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    • v.43 no.1
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    • pp.190-211
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    • 2010
  • The purpose of this paper is to provide the preliminary data for maintenance of old trees of natural monuments and establishing protection projects. Accordingly, growth conditions, location circumstances, environment and protection management were analyzed for old trees at 34 sites in Gyeongsangbuk-do. Tree forms, crown, trunk and trees' health, leaves volume were analyzed in survey sites. As the results, the growth of Picrasma quassioides in Songsadong, Andong-si and Catalpa ovata in Hongwon-ri, Cheongsong-gun were not in good condition, along with other old trees of 6 cities (Quercus variabilis in Susan-ri, Uljin-gun, Juniperus chinensis in Hujeong-ri, Uljin-gun, Pyrus ussuriensis in Ssangjeon-ri, Uljin-gun, Salix glandulosa var. pilosa in Deokchon-ri, Cheongdo-gun, Pinus densiflora for. multicaulis in Hwasan-ri, Mungyeong-si, Pinus densiflora in Daeha-ri, Mungyeong-si). Accoding to these results, protection project and consistent monitoring are necessary. The analysis results of growth conditions per location circumstances showed that the old trees in the small spaces with large traffic of people were in bad condition. Therefore, it is necessary to secure sufficient space and soil improvement for such trees. The analysis results of growth conditions per species showed that Zelkova serrata, Quercus spp., Pinus densiflora are in good condition while Salix koreensis and others, i.e. a singular tree, are in poor condition. Thus, relatively smaller individuals and separately situated trees need careful management in their protection. The analysis results of trunk's growth ratio showed that Pinus densiflora, Ginkgo biloba, Zelkova serrata are in good condition whereas Juniperus chinensis, Quercus variabilis, Salix koreensis are not. It is a recent trend that tree surgeries are gradually decreasing; there are a number of damaged trees with no maintenance/management, and several sites requiring mend trunks management and cabling, bracing in protection and management projects. Hence, in the future, local government and management organizations should plan for systematic management and establish a protection plan for the natural monument trees. Also, 12 sites were found to be in need to purchase lands in order to secure the growth spaces, and since most of these areas are available for purchase, it is needed to promote a budget and purchase those areas through persuasion of the owner. These areas seems crucial in order to maximize the effect of conservation efforts and budgets for the preservation and restoration of the old trees.