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http://dx.doi.org/10.14578/jkfs.2015.104.2.187

Genetic Monomorphism of the Natural Ilex cornuta Community at the Northern Range Limit in Buan, Jeollanam-do in Korea Revealed by AFLP Markers  

Hong, Kyung Nak (Division of Forest Genetic Resources, Korea Forest Research Institute)
Park, Yu Jin (Division of Forest Genetic Resources, Korea Forest Research Institute)
Lee, Jei Wan (Division of Forest Genetic Resources, Korea Forest Research Institute)
Kim, Young Mi (Division of Forest Genetic Resources, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.104, no.2, 2015 , pp. 187-192 More about this Journal
Abstract
Individuals in the population under a particular environmental condition influencing recurrently for a long time could locally adapted and local adaptation is of a fundamental importance in a breeding program, conservation activities of genetic resources or evolutionary biology. Plants at northern range limits have higher probability of expressing an adaptative genetic trait. The natural community at the northern range limit of Ilex cornuta (Chinese holly) in Buan, Jeollanam-do in Korea was composed of adults of 744 and seedlings of 211 (hereafter Community) and is designated as the Korean Natural Monument (No. 122) by the law. At two adjacent areas to Community, 85 (hereafter Plantation I) and 27 hollies (hereafter Plantation II) were planted respectively for preparations of the next generation. Eighty-five trees were sampled for genetic analysis in the three groups. Fifty-two (36%) of the total 143 amplicons were polymorphic from four AFLP primer combinations. A total of thirteen genotypes was identified and just one genotype was for 52 trees of Community. Seven and five genotypes were shown for Plantation I and II, respectively. There was no identical genotype between Community and Plantation (I or II) or between two plantation groups. Number of private loci was 2 for Community, 6 for Plantation I and 4 for Plantation II. We presumed their genetic backgrounds were quite different with one another and the plantation groups were made independently because they were different not only the genetic compositions but also their ages. Considering the genetic monomorphism by AFLP markers, observations of only male trees and asexual propagation as layerage or cuttage, the hollies in Community might be a genet by root suckering from a single male tree, not the results of selective removal of female trees for ornamental use in the past.
Keywords
chinese holly; genetic diversity; putative clone; clonality; genetic conservation;
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1 Bellard, C., Bertelsmeier, C., Leadley, P., Thuiller, W., and Courchamp, F. 2012. Impacts of climate change on the future of biodiversity. Ecology Letters 15: 365-377.   DOI
2 Bensch, S. and Akesson, M. 2005. Ten years of AFLP in ecology and evolution: why so few animals? Molecular Ecology 14: 2829-2914.
3 Cavigelli, M., Poulos, M., Lacey, E.P., and Mellon, G. 1986. Sexual dimorphism in temperate dioecious tree, Ilex montana (Aquifoliaceae). The American Midland Naturalist 115(2): 397-406.   DOI
4 Douhovnikoff, V. and Dodd, R.S. 2003. Intra-clonal variation and a similarity threshold for identification of clones: application to Salix exigua using AFLP molecular markers. Theoretical and Applied Genetics 106: 1307-1315.
5 Eckert, C.G., Samis, K.E., and Lougheed, S.C. 2008. Genetic variation across species' geographical ranges: the centralmarginal hypothesis and beyond. Molecular Ecology 17: 1170-1188.   DOI
6 Ellstrand, N.C. and Roose, M.L. 1987. Patterns of genotypic diversity in clonal plant species. American Journal of Botany 74(1): 123-131.   DOI
7 Fager, E.W. 1972. Diversity: a sampling study. American Journal of Botany 106(949): 293-310.
8 FRI (Forestry Research Institute). 1988. Illustrated Woody Plants of Korea. Samjeong Press. Seoul. pp. 496.
9 Geburek, T. and Konrad, H. 2008. Why the conservation of forest genetic resources has not worked. Conservation Biology 22(2): 267-274.   DOI
10 Gottlieb, A.M., Giberti, G.C., and Poggio, L. 2005. Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and ITS sequence data. American Journal of Botany 92(2): 352-369.   DOI
11 Hong, S.C. 2003. Studies on the System of Tree Cultivation for the Income Improvement in the Short-term Forest Products. Korean Ministry of Agriculture and Forestry. Seoul. pp. 292 (in Korean).
12 Knight, P.R., Eakes, D.J., Gilliam, C.H., and Tilt, K.M. 1993. Propagation container size and duration to transplant on growth of two Ilex species. Journal of Environmental Horticulture 11(4): 160-162.
13 Koh, M.H., Kim, Y.S., and Oh, H.K. 2005. Morphological characteristics of Chinese holly (Ilex cornuta) leaves in Korea. Korean Journal of Environment and Ecology 19(4): 348-357 (in Korean).
14 Kong, W.S., Lee, S.G., Yoon, K.H., and Park, H.N. 2011. Environmental characteristics of wind-whole and phytogeographical values. Journal of Environmental Impact Assessment 20(3): 381-395 (in Korean).
15 Kwon, H.J., Lee, J.H., Kim, M.Y., Lee, J.H., and Song, H.K. 2011. Vegetation structure and soil properties of Ilex cornuta population in Jeju Island. Korean Journal of Environment and Ecology 25(1): 10-16 (in Korean).
16 Mba, C. and Tohme, J. 2005. Use of AFLP markers in surveys of plant diversity. Methods in Enzymology 395: 177-201.   DOI
17 Lee, J.S. 1983. Studies on the natural distribution and ecology of Ilex cornuta Lindley et Pax. in Korea. Journal of Korean Forest Society 62: 24-42 (in Korean).
18 Leimu, R. and Fischer, M. 2008. A meta-analysis of local adaptation in plants. PLos One 3(12): e4010. doi:10.1371/journal.pone.0004010.   DOI
19 Lepping, G. and White, J.W. 2006. Conservation of peripheral plant populations in California. Madrono 53(3): 264-274.   DOI
20 Meudt, H.M. and Clarke, A.C. 2007. Almost forgotten or latest practice? AFLP applications, analyses and advances. Trends in Plant Science 12(3): 106-117.   DOI
21 Na, M.H., Lee, J.H., and Lee, J.K. 2010. A study on the present conditions of conservation and management of the natural monuments of Korea. Journal of Korean Institute of Traditional Landscape Architecture 28(2): 127-136 (in Korean).
22 Obeso, J.R. 1997. The induction of spinescence in European holly leaves by browsing ungulates. Plant Ecology 129: 149-156.   DOI
23 Oh, H.K., Han, Y.H., Lee, J.H., and Soh, M.S. 2011. A study on vascular plants of sites in the Byeonsanbando National Park. Journal of National Park Research 2(1): 6-18 (in Korean).
24 Park, C.M., Seo, B.S., Kim, K.H., Park, J.M., and Lim, S.J. 2000. Inhabitation environments and growth conditions of Ilex cornuta community in Pyonsanbando. Journal of Korean Institute of Traditional Landscape Architecture 18(1): 100-115 (in Korean).
25 Peakall, R. and Smouse, P.E. 2006. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Note 6: 288-295.   DOI
26 Son, S.W., Kim, J.H., Kim, Y.S., and Park, S.J. 2007. ITS sequence variations in populations Ilex cornuta (Aquifoliaceae). Korean Journal of Plant Taxonomy 37(2): 131-141 (in Korean).
27 Pielou, E.C. 1969. An Introduction to Mathematical Ecology. Wiley-Interscience. New York. pp. 286.
28 Richards, A.J. 1988. Male predominant sex rations in Holly (Ilex aquifolium L., Aquifoliaceae) and Roseroot (Rhodiola rosea L., Crassulaceae). Watsonia 17: 53-57.
29 Skou, A.T., Toneatto, F., and Kollmann, J. 2012. Are plant populations in expanding ranges made up of escaped cultivars? The case of Ilex aquifolium in Denmark. Plant Ecology 213: 1131-1144.   DOI
30 Sun, Y., Zhang, D., and Geng, F. 2010. Genetic diversity and taxon delineation of Ilex glabra using AFLP markers. Acta Horticulturae 859: 261-270.
31 Torimaru, T., Tomaru, N., Nishimura, N., and Yamamoto, S. 2003. Clonal diversity and genetic differentiation in Ilex leucoclada M. patches in an old-growth beech forest. Molecular Ecology 12: 809-818.   DOI
32 Vekemans, X., Beauwens, T., Lemaire, M., and Roldan-Ruiz, I. 2002. Data from amplified fragment length polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between the degree of homoplasy and fragment size. Molecular Ecology 11: 139-151.   DOI   ScienceOn
33 Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Friters, J. Pot, J. Paleman, M. Kuiper, and Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23(21): 4407-4414.   DOI
34 Whitlock, R., Hipperson, H., Mannarelli, M., Butlin, R.K., and Burke, T. 2008. An objective, rapid and reproducible method for scoring AFLP peak-height data that minimizes genotyping error. Molecular Ecology Resources 8: 725-735.   DOI
35 Yim, K.B. 1979. Variation of Genus Ilex in Korea and their ornamental values. Journal of Korean Forest Society 42: 1-38 (in Korean).
36 Wu, T., Li, Y., Tang, Q., and Wang, Z. 2008. Two unusual minor 18,19-seco-ursane glycosides from leaves of Ilex cornuta. Food Chemistry 111: 78-82.   DOI