• Animal cells and systems
• /
• v.16 no.2
• /
• pp.87-94
• /
• 2012

Nur77 is a member of the nuclear receptor 4A (NR4A) subgroup, which has been implicated in energy metabolism. Although Nur77 is found in adipose tissue, where TR4 plays a key role in lipid homeostasis, the role of Nur77 in adipogenesis is still controversial. Although the Nur77 responsive element (AAAGGTCA) is partially overlapped with TR4-binding sites (AGGTCA $n$ AGGTCA: $n$=0-6), the regulatory role of Nur77 in TR4 function associated with adipocyte biology remains unclear. Here, we found that Nur77 inhibits adipogenesis and TR4 transcriptional activity. Treatment with a Nur77 agonist, 1,1-bis(3'-indolyl)-1-($p$-anisyl)-methane, during 3T3-L1 adipocyte differentiation reduced adipogenesis. In reporter gene analysis, Nur77 specifically suppressed TR4 transcription activity but had little effect on $PPAR{\gamma}$ transcription activity. Consistently, Nur77 also suppressed TR4-induced promoter activity of the TR4 target gene PEPCK, which is known to be important for glyceroneogenesis in adipose tissue. Furthermore, Nur77 suppressed TR4 binding to TR4 response elements without direct interaction with TR4, suggesting that Nur77 may inhibit TR4 transcription activity via binding competition for TR4-binding sites. Furthermore, DIM-C-$pPhOCH_3$ substantially suppressed TR4-induced PEPCK expression in 3T3-L1 adipocytes. Together, our data demonstrate that Nur77 plays an inhibitory role in TR4-induced PEPCK expression in 3T3-L1 adipocytes.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.11
• /
• pp.6728-6738
• /
• 2014

This study investigated the relationship among pain, depression and activities of daily living in nursing home residents. Data was collected through a structured questionnaire survey of residents at 4 nursing homes in S city. The measures were the Numeric Rating Scale (NRS) and Discomfort Scale-Dementia of the Alzheimer Type (DS-DAT) for self-reported and observational pain, Geriatric Depression Scale Short Form-Korea Version (GDSSF-K) for depression, Barthel Activities of Daily Living (BADL) scale for ADL. The data were analyzed using the Pearson's correlation coefficient and hierarchical multiple regression using the SPSS/PC 20.0 program. The analysis results of the relationship of pain, depression and activities of daily living, both DS-DAT and NRS showed a significant negative correlation with the activities of daily living and a positive correlation with depression. Finally, gender, arthritis, depression, and activities of daily living explained 28.1% of the variance in pain using NRS, whereas depression and activities of daily living explained 21.5% of the variance in pain using DS-DAT. The results highlight the need to develop a pain management program that reduces the depression and promotes the activities of daily living in nursing home residents; hence, a differentiated approach as non-pharmacological interventions is required.

• Advances in environmental research
• /
• v.3 no.4
• /
• pp.355-365
• /
• 2014

The present study has been performed on one year old tree saplings of Azadirachta indica (L.), Cassia siamea (L.), Dalbergia sissoo (Roxb.), Eucalyptus rostrata (L.), Mangifera indica (L.) and Schyzygium cumini (L.) in order to assess the effect of exposure of $SO_2-NO_2$, alone and combination of two gases. Tree saplings have been exposed to an average of $495{\mu}g\;m^{-3}$ $SO_2$ and $105{\mu}g\;m^{-3}$ $NO_2$ for 40 d at the rate of $4hd^{-1}$ during 10:00 am to 01:00 pm in OTC. Total chlorophyll, specific leaf area (SLA), nitrate reductase (NR) activity, foliar protein, free proline content and free amino acids (AAs) of foliage have been the plant parameters, taken into consideration to evaluate the effect of gaseous exposure. Exposure of two gases has caused reduction in total chlorophyll content (P < 0.05, 0.01). Physiological and biochemical process has been seemed to be altered noticeable due to the combined effect of $SO_2+NO_2$ followed by $SO_2$ alone (P < 0.05, 0.01). $NO_2$ mediated stress has produced, stimulatory and inhibitory responses in tree saplings. Results reveal that tree saplings have been attempted to absorb the $NO_2$ through N assimilation pathway. E. rostrata, C. siamea have been emerged as moderate tolerant to $SO_2$ mediated stress followed by A. indica. Response pattern of S. cumini, M. indica and D. sissoo set them as good indicators of $SO_2-NO_2$ exposure. Effects of two gases on tree saplings have been found to be synergistic.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.34 no.1
• /
• pp.14-23
• /
• 2020

This study was conducted to evaluate the efficacy of Cheongawongagam on osteoporosis rat. A total of 35 rats were divided into seven groups; Normal control(SD-Nr), experimental control group(OVX-CTL), positive control group(OVX-17β-E2) and herb extracts group[Eucommia ulmoides(OVX-EU-E), Juglandis semen(OVX-JR-SE), Acanthopanax senticosus(OVX-AS-E) extract and Cheongawongagam extracts(OVX-JAEG-E)]. All control group, and herb extracts group were ovariectomized. After the 3 weeks recovery period, herb extract group were orally administered 200 mg / kg of the EU-E, JR-SE, AS-E and JAEG-E for 12 weeks. In the OVX-CTL, 17β-estradiol(E2) was administered subcutaneously on the back of the rats at a dose of 0.03 ug/sc. Their body weight, serum total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Leukotriene B4 (LTB4), calcium (Ca), estradiol, osteocalcin, and deoxypyridinoline (DPD) concentration were measured. Also, we investigated mRNA expression of inflammatory cytokine, MMP-2, MMP-9, and bone tissue. As a result, total cholesterol was significantly decreased in the OVX-AS-E and OVX-JAEG-E. ALP was significantly increased and osteocalcin, DPD was significantly decreased in OVX-JAEG-E. The expression of inflammatory mediators (TNF-α, IL-1β, LTB4, COX-2, NOS-2), inflammatory cytokines IL-1β and MMP-9 mRNA were significantly decreased in OVX-JAEG-E. Histologic examination of the femur showed that bone mineral density, and bone mass were increased and bone marrow were decreased in the OVX-JAEG-E group. The above experiment shows that cheongawongagam extract were effective in the prevention and treatment of osteoporosis.

• Journal of Applied Biological Chemistry
• /
• v.64 no.4
• /
• pp.333-341
• /
• 2021

In this study, to select strains suitable as microbial agent from among rhizosphere microorganisms present in rhizosphere soil and roots, the mineral solubilization ability, antifungal activity against 10 types of plant pathogenic fungi, and plant growth-promoting activity of rhizosphere microorganisms were evaluated. As a result, DDP346 was selected because it has solubilization ability of phosphoric acid, calcium carbonate, silicon, and zinc; nitrogen fixing ability; production ability of siderophore, indole-3-acetic acid, and aminocyclopropane-1-carboxylate deaminase; and antifungal activity against seven types of plant pathogenic fungi. DDP346 showed a 99.9% homology with Acinetobacter pittii DSM 21653 (NR_117621.1); phylogenetic analysis also revealed a close relationship with Acinetobacter pittii based on the 16S rRNA base sequence. The growth conditions of DDP346 were identified as temperatures in the range of 10-40 ℃, pH in the range of 5-11, and salt concentrations in the range of 0-5%. In addition, a negative correlation coefficient (r² = -0.913, p <0.01) was shown between pH change and the solubilized phosphoric acid content of Acinetobacter sp. DDP346, and this is assumed to be due to the organic acid generated during culture. Consequently, through the evaluation of its mineral solubilization ability, antifungal activity against plant pathogenic fungi, and plant growth-promoting activity, the potential for the utilization of Acinetobacter sp. DDP346 as a multi-purpose microbial agent is presented.

• Polymer(Korea)
• /
• v.36 no.2
• /
• pp.235-244
• /
• 2012

A thiazole type accelerator MBT (2-mercapto benzothiazole) was added into silica filled natural rubber (NR) compound with various concentrations (0, 1, 2, 3, 4 phr). The effects of MBT on the cure rate, mechanical property, degree of rubber-filler interaction (${\alpha}_F$), crosslinking density, and viscoelastic property ($tan{\delta}$) were investigated. As accelerator concentration increased, the $t_{s2}$ and $t_{90}$ decreased and the crosslinking density and modulus at 300% elongation increased. The tensile strength and elongation increased up to 3 phr and no further increased at 4 phr. The $tan{\delta}$ value measured at room temperature was higher than that of the $70^{\circ}C$. The ${\alpha}_F$ value was not affected by the addition of MBT. The mechanisms for the vulcanization rate were reviewed.

• The Journal of Korean Medicine
• /
• v.31 no.2
• /
• pp.19-35
• /
• 2010

Objectives: This study was carried out to find the effects of Changchuldoin-tanggamibang (hereinafter referred to CDIT) on the inhibition of arthritis induced by collagen on DBA/1J mouse. Methods: The experimental mice were divided into four groups: normal group (Nr), control group (CIA-CT), methotrexate group (CIA-MTX), and Changchuldoin-tanggamibang group (CIA-CDIT). Cytotoxicity, hepatotoxicity, arthritis index, value of immunocytes in draining lymph node and paw joint, and rheumatoid factor (IgG, IgM) in serum were measured in vivo. Results: 1. Cytotoxicity against hFCs was not shown in any concentration. 2. Hepatotoxicity was low in the CDIT-treated group compared with the MTX group. 3. The arthritis index decreased significantly. 4. In total cell counts of DLN and paw joint, the cells in DLN increased significantly while there was a significant decrease in paw joint. 5. In lymph nodes, CD19+, CD3+, CD4+, CD8+, CD3+/CD8+, CD3+/CD69+, CD4+/CD25+, CD3+/CD49b+, and CD4+/CD44+ cells increased significantly, while B220+/CD23+, and CD11c+/MHCII+ cells decreased significantly. 6. In joints, CD3+, CD4+, CD4+/CD25+, and CD11b+/Gr-1+ cells decreased significantly. 7. The level of IgG decreased and the level of IgM significantly decreased compared with the control. 8. Anti-collagen II in serum decreased compared with the control. 9. Around the joint of the CDIT group, infiltration of inflammation, synovial hyperplasia, invasion of cytokine, of cartilage, deposition of collagen and synovial injury decreased compared with the control in histopathologic observation (HE, MT staining). Conclusions: Comparison of the results for this study showed that CDIT had immunomodulatory effects. We expect that CDIT could be used as a effective drug for not only rheumatoid arthritis but also another auto-immune diseases. Therefore, we have to survey continuously, looking for effective substances and mechanisms in the future.

• Biomolecules & Therapeutics
• /
• v.24 no.2
• /
• pp.191-198
• /
• 2016

The vitamin D receptor (VDR) is a member of the nuclear receptor (NR) superfamily. The VDR binds to active vitamin $D_3$ metabolites, which stimulates downstream transduction signaling involved in various physiological activities such as calcium homeostasis, bone mineralization, and cell differentiation. Quercetin is a widely distributed flavonoid in nature that is known to enhance transactivation of VDR target genes. However, the detailed molecular mechanism underlying VDR activation by quercetin is not well understood. We first demonstrated the interaction between quercetin and the VDR at the molecular level by using fluorescence quenching and saturation transfer difference (STD) NMR experiments. The dissociation constant ($K_d$) of quercetin and the VDR was $21.15{\pm}4.31{\mu}M$, and the mapping of quercetin subsites for VDR binding was performed using STD-NMR. The binding mode of quercetin was investigated by a docking study combined with molecular dynamics (MD) simulation. Quercetin might serve as a scaffold for the development of VDR modulators with selective biological activities.

• Elastomers and Composites
• /
• v.36 no.4
• /
• pp.255-261
• /
• 2001

This study was related with the effect of elevated temperature on the tensile strength of edge-cut samples. There was a different tensile strength behavior of uncut samples and pre-cut samples under different test temperatures. Tensile strength of uncut sample decreases with increasing test temperature. When pro-cut size(C) is larger than critical cut size($C_{cr}$), tensile strength or pre-cut specimen at $80^{\circ}C$ is higher than that of pre-cut specimen at room temperature (RT). Test specimens under $80^{\circ}C$ condition exhibited more secondary cracks at the crack tip region compared to room temperature conditions. However, secondary cracks of pre-cut specimens are not clearly developed at $110^{\circ}C$. Differences in tensile strength induced by different test temperature seem to be responsible for the strain-induced crystallization and micro-cracking patterns.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.13 no.1
• /
• pp.227-235
• /
• 2012

The purpose of this study was to analysis the effect of form of outsole on lower leg Electromyography (EMG) during gait. The subject were 11 women(mean age $47.33{\pm}4.4$ years, height $158.00{\pm}3.3$cm, weight $59.50{\pm}6.1$, body mass index $23.80{\pm}1.8kg/m^2$), who had no serious foot musculoskeletal disease within3years prior to study. The vastus medialis, gastrocnemius, semitendinosus, tibialis anterior were compared during gait with 2 types of shoe(complex function double rocker sole, negative-heel rocker sole) by using pair t-test(p<.05). The vastus medialis, gastrocnemius, semitendinosus were not found to be significantly different among the 2 types of shoe but tibialis anterior were found(p<.05). The complex function double rocker sole shoe caused a significant change in energy expenditure compared to the negative-heel rocker sole shoe.