Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Cheongawongagam Extract on the Ovariectomized Rat Model of Osteoporosis

청아원가감 추출물이 난소적출로 유발된 흰쥐의 골다공증 모델이 미치는 영향

  • Yun, Gee Won (Department of Acupuncture & Moxibustion Medicine, Cheonan Korean Medical Hospital of Daejeon University) ;
  • Lee, Hyun (Department of Acupuncture & Moxibustion Medicine, Cheonan Korean Medical Hospital of Daejeon University)
  • 윤지원 (대전대학교 천안한방병원 침구의학과) ;
  • 이현 (대전대학교 천안한방병원 침구의학과)
  • Received : 2019.11.02
  • Accepted : 2020.01.31
  • Published : 2020.02.25
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Abstract

This study was conducted to evaluate the efficacy of Cheongawongagam on osteoporosis rat. A total of 35 rats were divided into seven groups; Normal control(SD-Nr), experimental control group(OVX-CTL), positive control group(OVX-17β-E2) and herb extracts group[Eucommia ulmoides(OVX-EU-E), Juglandis semen(OVX-JR-SE), Acanthopanax senticosus(OVX-AS-E) extract and Cheongawongagam extracts(OVX-JAEG-E)]. All control group, and herb extracts group were ovariectomized. After the 3 weeks recovery period, herb extract group were orally administered 200 mg / kg of the EU-E, JR-SE, AS-E and JAEG-E for 12 weeks. In the OVX-CTL, 17β-estradiol(E2) was administered subcutaneously on the back of the rats at a dose of 0.03 ug/sc. Their body weight, serum total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Leukotriene B4 (LTB4), calcium (Ca), estradiol, osteocalcin, and deoxypyridinoline (DPD) concentration were measured. Also, we investigated mRNA expression of inflammatory cytokine, MMP-2, MMP-9, and bone tissue. As a result, total cholesterol was significantly decreased in the OVX-AS-E and OVX-JAEG-E. ALP was significantly increased and osteocalcin, DPD was significantly decreased in OVX-JAEG-E. The expression of inflammatory mediators (TNF-α, IL-1β, LTB4, COX-2, NOS-2), inflammatory cytokines IL-1β and MMP-9 mRNA were significantly decreased in OVX-JAEG-E. Histologic examination of the femur showed that bone mineral density, and bone mass were increased and bone marrow were decreased in the OVX-JAEG-E group. The above experiment shows that cheongawongagam extract were effective in the prevention and treatment of osteoporosis.

Keywords

References

  1. Korean society for bone and mineral research. Osteoporosis 3 edition. Seoul : Hanmi publishing; 2006. 11-7, 205-15.
  2. Yoo JH, Moon SH, Ha YC, Lee DY, Gong HS, Park SY, et al. Osteoporotic fracture: 2015 position statement of the Korean society for bone and mineral research. J bone Metab. 2015;22(4):175-81. https://doi.org/10.11005/jbm.2015.22.4.175
  3. Lee SR, Kim SR, Chung KH, Ko DO, Cho SH, Ha YC, et al. Mortality and activity after hip fracture: a prospective study. J Korean Orthop Assoc. 2005;40(4):423-7. https://doi.org/10.4055/jkoa.2005.40.4.423
  4. Kim YS. Strategies for prevention of elderly hip fracture. J Korean Med Assoc. 2016;59(8):631-6. https://doi.org/10.5124/jkma.2016.59.8.631
  5. Kim JH. Reviews of recent research on the osteoporosis - is mainly dependent on the oriental medicine clinical and experimental study. J Korean Acupunct Moxibustion Soc. 1998;15(2):437-54.
  6. The Korean orthopaedic association. Orthopaedic. Seoul : Choesin publishing ; 1999.
  7. Seo MS, Kim HC, Choo WJ, Jeong SY, Kim SJ, Choi JU, et al. The Review on the Study of Osteoporosis in Korean Medicine Journals. J Korea CHUNA Man Med Spine Nerves. 2013;8(2):67-78.
  8. Kim MB, Kim SS, Chung SH. A Literature Review of Herbal Medicines on Osteoporosis Studies-Reviewing Articles Published after Year 2000. J Korean Med Rehabil. 2010;20(1):91-107.
  9. J Huh. Dogneui Bogam. Seoul : Dongeuibogam publishing; 2005.
  10. Kim SH, Jeong JC. Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan. J Korean Med. 2002;23(4).
  11. Yun GW, Kang JH, Lee H. Effects of Korean herbal medicine (Cheong-A-Won) for treatment of bone mineral density in women with osteoporosis: A randomized, double blind, placebo controlled trial. Eur J Integr Med. 2018;20:84-9. https://doi.org/10.1016/j.eujim.2018.04.009
  12. Xu Y, Zhao Y, Xie J, Sheng X, Li Y, Zhang Y. The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOF/MS. Evidence-Based Complement Altern Med. 2017;2017.
  13. HB Pyeonchanwiwonhoe. Bonchohak. Seoul : Yeongrimsa. 2004;325-6, 603-4, 616-7.
  14. Chung HY. Osteoporosis diagnosis and treatment 2007. Endocrinol Metab. 2008;23(2):76-108.
  15. Yang KH. Ageing society and osteoporosis. J Korean Med Assoc. 2016;59(11):833-5. https://doi.org/10.5124/jkma.2016.59.11.833
  16. Park SH, Lee K. Prediction for the spatial distribution of medical facilities complying with the changes in the population structure in Korea. J Geol Soc Korea. 2018;53(53):371-85.
  17. Cosman F, de Beur SJ, LeBoff MS, Lewiecki EM, Tanner B, Randall S, et al. Clinician's guide to prevention and treatment of osteoporosis. Osteoporos Int. 2014;25(10):2359-81. https://doi.org/10.1007/s00198-014-2794-2
  18. Abrahamsen B. Bisphosphonate adverse effects, lessons from large databases. Curr Opin Rheumatol. 2010;22(4):404-9. https://doi.org/10.1097/BOR.0b013e32833ad677
  19. MacLean C, Newberry S, Maglione M, McMahon M, Ranganath V, Suttorp M, et al. Systematic review: comparative effectiveness of treatments to prevent fractures in men and women with low bone density or osteoporosis. Ann Intern Med. 2008;148(3):197-213. https://doi.org/10.7326/0003-4819-148-3-200802050-00198
  20. Do HK. Donguisingyehak, Seoul. 1991;1144-51.
  21. Kim MS, Seo BI, Kwak MA, Ji SY. Effect of Chungajihwangtang on osteoporosis in ovariectomized rats. Korea J Herbol. 2003;18(2):49.
  22. Kang JH, Choi JY, Yoon KS, Lee H. The analysis of 278 cases who were medicated with Cheonga-won. J Korean Acupunct Mox Med Sci. 2011;28(1):93-100.
  23. Zhang R, Liu ZG, Li C, Hu SJ, Liu L, Wang JP, et al. Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone. 2009;45(3):553-9. https://doi.org/10.1016/j.bone.2008.08.127
  24. Jung YT, Choi YH, Song JH, Lee CH, Lee MS, Jang SJ, et al. Effect of water extract of Eucommiae cortex in RANKL-induced osteoclast differentiation. J Physiol Pathol Korean Med. 2009;23(3):613-8.
  25. Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P. Walnut extract (Juglans regia L.) and its component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity in the cell line KS483. Br J Nutr. 2008;99(4):715-22. https://doi.org/10.1017/S0007114507837421
  26. Hwang YC, Jeong IK, Ahn KJ, Chung HY. The effects of Acanthopanax senticosus extract on bone turnover and bone mineral density in Korean postmenopausal women. J Bone Miner Metab. 2009;27(5):584-90. https://doi.org/10.1007/s00774-009-0093-3
  27. Funk JL, Frye JB, Wright LE, Timmermann BN. Effects of Ginger (Zingiber officialis L) on Inflammation-Induced Bone Loss. Federation of American Societies for Experimental Biology; 2012.
  28. Fan JZ, Yang X, Bi ZG. The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells. Brazilian J Med Biol Res. 2015;48(7):637-43. https://doi.org/10.1590/1414-431X20154494
  29. Kalu DN, Arjmandi BH, Liu CC, Salih MA, Birnbaum RS. Effects of ovariectomy and estrogen on the serum levels of insulin-like growth factor-I and insulin-like growth factor binding protein-3. Bone Miner. 1994;25(2):135-48. https://doi.org/10.1016/S0169-6009(08)80255-3
  30. Stevenson JC, Crook D, Godsland IF. Influence of age and menopause on serum lipids and lipoproteins in healthy women. Atherosclerosis. 1993;98(1):83-90. https://doi.org/10.1016/0021-9150(93)90225-J
  31. Datta HK, Ng WF, Walker JA, Tuck SP, Varanasi SS. The cell biology of bone metabolism. J Clin Pathol. 2008;61(5):577-87. https://doi.org/10.1136/jcp.2007.048868
  32. Naylor K, Eastell R. Bone turnover markers: use in osteoporosis. Nat Rev Rheumatol. 2012;8(7):379. https://doi.org/10.1038/nrrheum.2012.86
  33. Yeh JK, Aloia JF, Barilla M-L. Effects of $17{\beta}$-estradiol replacement and treadmill exercise on vertebral and femoral bones of the ovariectomized rat. Bone Miner. 1994;24(3):223-34. https://doi.org/10.1016/S0169-6009(08)80139-0
  34. Mundy GR. Osteoporosis and inflammation. Nutr Rev. 2007;65(suppl_3):S147-51. https://doi.org/10.1111/j.1753-4887.2007.tb00353.x
  35. Pino AM, Rios S, Astudillo P, Fernandez M, Figueroa P, Seitz G, et al. Concentration of adipogenic and proinflammatory cytokines in the bone marrow supernatant fluid of osteoporotic women. J Bone Miner Res. 2010;25(3):492-8. https://doi.org/10.1359/jbmr.090802
  36. Hammett-Stabler CA. The use of biochemical markers in osteoporosis. Clin Lab Med. 2004;24(1):175-97. https://doi.org/10.1016/j.cll.2004.01.013
  37. Notelovitz M. Osteoporosis: screening, prevention, and management. Fertil Steril. 1993;59(4):707-25. https://doi.org/10.1016/S0015-0282(16)55848-8
  38. Kim W. Recent research of biochemical bone markers measurement in osteoporosis and clinical utility. Graduate School of Seoul National University; 2015.
  39. Choi SH, Lee SY, Kim YJ. The pattern of urinary deoxypyridinoline and serum osteocalcin across menopausal transition in women. J Korean Acad Fam Med. 2000;21(12):1552-9.
  40. Nyman JS, Lynch CC, Perrien DS, Thiolloy S, O'Quinn EC, Patil CA, et al. Differential effects between the loss of MMP-2 and MMP-9 on structural and tissue-level properties of bone. J Bone Miner Res. 2011;26(6):1252-60. https://doi.org/10.1002/jbmr.326