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http://dx.doi.org/10.3839/jabc.2021.045

Biochemical and cultural characteristics of mineral-solubilizing Acinetobacter sp. DDP346  

Kim, Hee Sook (Food Research Center, Angel Co., Ltd.)
Lee, Song Min (Food Research Center, Angel Co., Ltd.)
Oh, Ka-Yoon (Food Research Center, Angel Co., Ltd.)
Kim, Ji-Youn (Food Research Center, Angel Co., Ltd.)
Lee, Kwang Hui (Food Research Center, Angel Co., Ltd.)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, Silla University)
Jang, Jeong Su (Food Research Center, Angel Co., Ltd.)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.4, 2021 , pp. 333-341 More about this Journal
Abstract
In this study, to select strains suitable as microbial agent from among rhizosphere microorganisms present in rhizosphere soil and roots, the mineral solubilization ability, antifungal activity against 10 types of plant pathogenic fungi, and plant growth-promoting activity of rhizosphere microorganisms were evaluated. As a result, DDP346 was selected because it has solubilization ability of phosphoric acid, calcium carbonate, silicon, and zinc; nitrogen fixing ability; production ability of siderophore, indole-3-acetic acid, and aminocyclopropane-1-carboxylate deaminase; and antifungal activity against seven types of plant pathogenic fungi. DDP346 showed a 99.9% homology with Acinetobacter pittii DSM 21653 (NR_117621.1); phylogenetic analysis also revealed a close relationship with Acinetobacter pittii based on the 16S rRNA base sequence. The growth conditions of DDP346 were identified as temperatures in the range of 10-40 ℃, pH in the range of 5-11, and salt concentrations in the range of 0-5%. In addition, a negative correlation coefficient (r2 = -0.913, p <0.01) was shown between pH change and the solubilized phosphoric acid content of Acinetobacter sp. DDP346, and this is assumed to be due to the organic acid generated during culture. Consequently, through the evaluation of its mineral solubilization ability, antifungal activity against plant pathogenic fungi, and plant growth-promoting activity, the potential for the utilization of Acinetobacter sp. DDP346 as a multi-purpose microbial agent is presented.
Keywords
Acinetobacter sp.; Microbial agent; Mineral solubilization; Plant growth promoting rhizobacteria;
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