Browse > Article
http://dx.doi.org/10.15188/kjopp.2020.02.34.1.14

Effect of Cheongawongagam Extract on the Ovariectomized Rat Model of Osteoporosis  

Yun, Gee Won (Department of Acupuncture & Moxibustion Medicine, Cheonan Korean Medical Hospital of Daejeon University)
Lee, Hyun (Department of Acupuncture & Moxibustion Medicine, Cheonan Korean Medical Hospital of Daejeon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.34, no.1, 2020 , pp. 14-23 More about this Journal
Abstract
This study was conducted to evaluate the efficacy of Cheongawongagam on osteoporosis rat. A total of 35 rats were divided into seven groups; Normal control(SD-Nr), experimental control group(OVX-CTL), positive control group(OVX-17β-E2) and herb extracts group[Eucommia ulmoides(OVX-EU-E), Juglandis semen(OVX-JR-SE), Acanthopanax senticosus(OVX-AS-E) extract and Cheongawongagam extracts(OVX-JAEG-E)]. All control group, and herb extracts group were ovariectomized. After the 3 weeks recovery period, herb extract group were orally administered 200 mg / kg of the EU-E, JR-SE, AS-E and JAEG-E for 12 weeks. In the OVX-CTL, 17β-estradiol(E2) was administered subcutaneously on the back of the rats at a dose of 0.03 ug/sc. Their body weight, serum total cholesterol, triglyceride, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), Leukotriene B4 (LTB4), calcium (Ca), estradiol, osteocalcin, and deoxypyridinoline (DPD) concentration were measured. Also, we investigated mRNA expression of inflammatory cytokine, MMP-2, MMP-9, and bone tissue. As a result, total cholesterol was significantly decreased in the OVX-AS-E and OVX-JAEG-E. ALP was significantly increased and osteocalcin, DPD was significantly decreased in OVX-JAEG-E. The expression of inflammatory mediators (TNF-α, IL-1β, LTB4, COX-2, NOS-2), inflammatory cytokines IL-1β and MMP-9 mRNA were significantly decreased in OVX-JAEG-E. Histologic examination of the femur showed that bone mineral density, and bone mass were increased and bone marrow were decreased in the OVX-JAEG-E group. The above experiment shows that cheongawongagam extract were effective in the prevention and treatment of osteoporosis.
Keywords
Osteoporosis; Eucommia ulmoides; Juglandis semen; Acanthopanax senticosus; Cheongawongagam extract;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Korean society for bone and mineral research. Osteoporosis 3 edition. Seoul : Hanmi publishing; 2006. 11-7, 205-15.
2 Yoo JH, Moon SH, Ha YC, Lee DY, Gong HS, Park SY, et al. Osteoporotic fracture: 2015 position statement of the Korean society for bone and mineral research. J bone Metab. 2015;22(4):175-81.   DOI
3 Lee SR, Kim SR, Chung KH, Ko DO, Cho SH, Ha YC, et al. Mortality and activity after hip fracture: a prospective study. J Korean Orthop Assoc. 2005;40(4):423-7.   DOI
4 Kim YS. Strategies for prevention of elderly hip fracture. J Korean Med Assoc. 2016;59(8):631-6.   DOI
5 Kim JH. Reviews of recent research on the osteoporosis - is mainly dependent on the oriental medicine clinical and experimental study. J Korean Acupunct Moxibustion Soc. 1998;15(2):437-54.
6 The Korean orthopaedic association. Orthopaedic. Seoul : Choesin publishing ; 1999.
7 Seo MS, Kim HC, Choo WJ, Jeong SY, Kim SJ, Choi JU, et al. The Review on the Study of Osteoporosis in Korean Medicine Journals. J Korea CHUNA Man Med Spine Nerves. 2013;8(2):67-78.
8 Kim MB, Kim SS, Chung SH. A Literature Review of Herbal Medicines on Osteoporosis Studies-Reviewing Articles Published after Year 2000. J Korean Med Rehabil. 2010;20(1):91-107.
9 J Huh. Dogneui Bogam. Seoul : Dongeuibogam publishing; 2005.
10 Kim SH, Jeong JC. Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan. J Korean Med. 2002;23(4).
11 Yun GW, Kang JH, Lee H. Effects of Korean herbal medicine (Cheong-A-Won) for treatment of bone mineral density in women with osteoporosis: A randomized, double blind, placebo controlled trial. Eur J Integr Med. 2018;20:84-9.   DOI
12 Xu Y, Zhao Y, Xie J, Sheng X, Li Y, Zhang Y. The Evaluation of Toxicity Induced by Psoraleae Fructus in Rats Using Untargeted Metabonomic Method Based on UPLC-Q-TOF/MS. Evidence-Based Complement Altern Med. 2017;2017.
13 HB Pyeonchanwiwonhoe. Bonchohak. Seoul : Yeongrimsa. 2004;325-6, 603-4, 616-7.
14 Chung HY. Osteoporosis diagnosis and treatment 2007. Endocrinol Metab. 2008;23(2):76-108.
15 Yang KH. Ageing society and osteoporosis. J Korean Med Assoc. 2016;59(11):833-5.   DOI
16 Park SH, Lee K. Prediction for the spatial distribution of medical facilities complying with the changes in the population structure in Korea. J Geol Soc Korea. 2018;53(53):371-85.
17 Do HK. Donguisingyehak, Seoul. 1991;1144-51.
18 Cosman F, de Beur SJ, LeBoff MS, Lewiecki EM, Tanner B, Randall S, et al. Clinician's guide to prevention and treatment of osteoporosis. Osteoporos Int. 2014;25(10):2359-81.   DOI
19 Abrahamsen B. Bisphosphonate adverse effects, lessons from large databases. Curr Opin Rheumatol. 2010;22(4):404-9.   DOI
20 MacLean C, Newberry S, Maglione M, McMahon M, Ranganath V, Suttorp M, et al. Systematic review: comparative effectiveness of treatments to prevent fractures in men and women with low bone density or osteoporosis. Ann Intern Med. 2008;148(3):197-213.   DOI
21 Kim MS, Seo BI, Kwak MA, Ji SY. Effect of Chungajihwangtang on osteoporosis in ovariectomized rats. Korea J Herbol. 2003;18(2):49.
22 Kang JH, Choi JY, Yoon KS, Lee H. The analysis of 278 cases who were medicated with Cheonga-won. J Korean Acupunct Mox Med Sci. 2011;28(1):93-100.
23 Fan JZ, Yang X, Bi ZG. The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells. Brazilian J Med Biol Res. 2015;48(7):637-43.   DOI
24 Zhang R, Liu ZG, Li C, Hu SJ, Liu L, Wang JP, et al. Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone. 2009;45(3):553-9.   DOI
25 Jung YT, Choi YH, Song JH, Lee CH, Lee MS, Jang SJ, et al. Effect of water extract of Eucommiae cortex in RANKL-induced osteoclast differentiation. J Physiol Pathol Korean Med. 2009;23(3):613-8.
26 Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P. Walnut extract (Juglans regia L.) and its component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity in the cell line KS483. Br J Nutr. 2008;99(4):715-22.   DOI
27 Hwang YC, Jeong IK, Ahn KJ, Chung HY. The effects of Acanthopanax senticosus extract on bone turnover and bone mineral density in Korean postmenopausal women. J Bone Miner Metab. 2009;27(5):584-90.   DOI
28 Funk JL, Frye JB, Wright LE, Timmermann BN. Effects of Ginger (Zingiber officialis L) on Inflammation-Induced Bone Loss. Federation of American Societies for Experimental Biology; 2012.
29 Kalu DN, Arjmandi BH, Liu CC, Salih MA, Birnbaum RS. Effects of ovariectomy and estrogen on the serum levels of insulin-like growth factor-I and insulin-like growth factor binding protein-3. Bone Miner. 1994;25(2):135-48.   DOI
30 Stevenson JC, Crook D, Godsland IF. Influence of age and menopause on serum lipids and lipoproteins in healthy women. Atherosclerosis. 1993;98(1):83-90.   DOI
31 Datta HK, Ng WF, Walker JA, Tuck SP, Varanasi SS. The cell biology of bone metabolism. J Clin Pathol. 2008;61(5):577-87.   DOI
32 Notelovitz M. Osteoporosis: screening, prevention, and management. Fertil Steril. 1993;59(4):707-25.   DOI
33 Naylor K, Eastell R. Bone turnover markers: use in osteoporosis. Nat Rev Rheumatol. 2012;8(7):379.   DOI
34 Yeh JK, Aloia JF, Barilla M-L. Effects of $17{\beta}$-estradiol replacement and treadmill exercise on vertebral and femoral bones of the ovariectomized rat. Bone Miner. 1994;24(3):223-34.   DOI
35 Mundy GR. Osteoporosis and inflammation. Nutr Rev. 2007;65(suppl_3):S147-51.   DOI
36 Pino AM, Rios S, Astudillo P, Fernandez M, Figueroa P, Seitz G, et al. Concentration of adipogenic and proinflammatory cytokines in the bone marrow supernatant fluid of osteoporotic women. J Bone Miner Res. 2010;25(3):492-8.   DOI
37 Hammett-Stabler CA. The use of biochemical markers in osteoporosis. Clin Lab Med. 2004;24(1):175-97.   DOI
38 Kim W. Recent research of biochemical bone markers measurement in osteoporosis and clinical utility. Graduate School of Seoul National University; 2015.
39 Choi SH, Lee SY, Kim YJ. The pattern of urinary deoxypyridinoline and serum osteocalcin across menopausal transition in women. J Korean Acad Fam Med. 2000;21(12):1552-9.
40 Nyman JS, Lynch CC, Perrien DS, Thiolloy S, O'Quinn EC, Patil CA, et al. Differential effects between the loss of MMP-2 and MMP-9 on structural and tissue-level properties of bone. J Bone Miner Res. 2011;26(6):1252-60.   DOI