The objective of the present work was to determine the corresponding uptake and assimilation of ${NO_3}^-$ in roots and shoots of leafy radish by applying of mixed amino acids (MAA). The amino acids used in this experiment were alanine (Ala), ${\beta}-alanine\;({\beta}-Ala)$, aspartic acid (Asp), asparagines (Asn), glutamic acid (Glu), glutamine (Gln), and glycine (Gly). Leafy radish was grown by conventional fertilization with macro- and micronutrients under controlled conditions. The 15-day-old seedlings were treated 0, 0.3 and 3.0 mM of MAA containing 5 mM ${NO_3}^-$ in growth medium. Nitrate uptake was determined by following ${NO_3}^-$ depletion from the uptake solution. The activity of the enzymes related to the process of ${NO_3}^-$ reduction (NR: nitrate reductase; NiR: nitrite reductase; GS: glutamine synthetase) and the content of ${NO_2}^-\;and\;{ND_3}^-$ were analyzed in shoots and roots. The results of this study showed that ${NO_3}^-$ uptake was inhibited 38% with treatment of 0.3 mM of MAA. However, there was more than three times increase of N03- uptake in 3.0 mM MAA. In addition, the enzymatic activities were positively affected by the high MAA rate. Finally, the ${NO_3}^-$ content was increased slightly both in shoots and roots of leafy radish by MAA treatments.
This study was carried out to determine the possibility of making fiberboard through the dry forming process, utilizing the tannin-like material of the Asplund pulp from the silk worm oak as a binder. The fiberboard was made through paraformaldehyde treatment, spray of NaOH solution and adjustment of mat moisture content, by hot-pressing at $220^{\circ}C$, and 50-6-50kg/$cm^2$ pressure with 6-min. (0.6-1.8-3.6) three-stage pressing cycle. The results are summarized as follows: 1. The modulus of rupture value of fiberboard treated with NaOH solution was greater than that of untreated. The value was increased in proportion to the paraformaldehyde content from I to 5%, but there was no increasing between 5 and 7% paraformaldehyde content. The value became higher along with the increase of mat moisture content from 15% to 25%. 2. The water adsorption of fiberboard treated with NaOH solution was lower than that of untreated. The value was de"creased in proportion to the paraformaldehyde content from 1 to 5%, but there was no decreasing between 5 and 7% paraformaldehyde content.. 3. A good quality fiberboard (modulus of rupture value of about 250kg/$cm^2$) was made through 5% paraformaidehyde treatment and 3% treatment of 10% NaOH solution, when the mat moisture content was 25%. was 25%.
Kwon, Gi Jin;Lee, Bo Ah;Byun, Chae Ho;Nam, Jong Min;Kim, Jae Geun
Journal of the Korean Society of Environmental Restoration Technology
/
v.9
no.1
/
pp.72-88
/
2006
The optimal environmental ranges of the establishment phase for the distribution of Zizania latifolia and Typha angustifolia was determined to develop a set of basic data and criteria of planting substrate for the restoration, conservation and management of wetlands. The study was carried at 17 wetlands in the Kyunggi-do and Gyeongsangnam-do region where inland wetlands place intensively in June, 2005. Total 127 quadrats were sets in growing areas of Zizania latifolia and Typha angustifolia. $NO_3-N$, K, Ca, Mg and Na in the water variables and soil texture, LOI (loss on ignition), soil pH and soil conductivity in the soil variables were analyzed. The optimal range of water depth for the distribution of Zizania latifolia was -5~39cm, $NO_3-N$ content of water was <0.01~0.19ppm, K content of water was 0.1~5.9ppm, Ca content of water 0.5~44.9ppm, Mg content of water was 1.2~11.9ppm, Na content of water 3.4~29.9ppm, water conductivity was 48~450${\mu}S$/cm, respectively. The optimal range of LOI for the distribution of Zizania latifolia was 1.7~11.9%, soil conductivity was 25.5~149.9${\mu}S$/cm, respectively. The optimal range of water depth for the distribution of Typha angustifolia was -20~24cm, $NO_3-N$ content of water was <0.01~0.19ppm, K content of water was 0.2~2.9ppm, Ca content of water 0.6~19.9ppm, Mg content of water was 0.2~5.9ppm, Na content of water 3.5~19.9ppm, water conductivity was 96~450${\mu}S$/cm, respectively. The optimal range of LOI for the distribution of Typha angustifolia was 2.4~15.9%, soil conductivity was 17.6~149.9${\mu}S$/cm, respectively. The optimal soil texture were loam, silt loam and sandy loam in both species. The lower water depth (-20~40cm) is appropriate to increase biodiversity in both species dominated community and it is better to maintain water depth of 40~100cm for water purification. Both species appear frequently in the soil with high silt content.
Journal of The Korean Society of Grassland and Forage Science
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v.13
no.4
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pp.278-285
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1993
This experiment was conducted to investigate the productivity in permanent orchardgrass grassland according to the urea fertilization levels of 0, 100, 200 kg N/ha and the animal excreta fertilization levels of 0. 40, 80, 160 kg N/ha on the basis of N contents respectively and NO$_3$-N content of the soil. The results were obtained as follows; 1. The productivity of grassland was significantly increased by the increase of the excreta fertilization levels until the urea-N level fertilization of 100 kg/ha but repressed and reduced by the increase to 160 kg/ha level of the animal excreta fertilization at urea-N fertilization level of 200 kg/ha. 2. Averaged seasonal productivity of the animal excreta N was decreased in first and third cutting time and was increased in second cutting time according to the increase of urea-N fertilization. 3. The increase of urea-N and animal excreta N increased the content of crude protein but the fertilization effect of urea-N was higher than that of animal excreta N. 4. The total yield of N was continuously increased at both of the urea-N and animal excreta N but the pure yield of N was decreased according to the increase of N fertilization and showed -63.1 kg/ha at the fertilization of the urea-N of 200 kg N/ha and animal excreta N of 160 kg/ha. 5. NO$_3$-N content of the soil of orchardgrass glassland established one year previously was no difference among the N variety and fertilization levels but was increased to the order of summer, autumn and winter. The highest content of NO$_3$-N was measured in winter with the value of 34.9 ppm.
Objective : To explore the histological feature of the cervical disc degeneration in patients with degenerative ossification (DO) and its potential mechanisms. Methods : A total of 96 surgical segments, from cervical disc degenerative disease patients with surgical treatment, were divided into ossification group (group O, n=46) and non-ossification group (group NO, n=50) based on preoperative radiological exams. Samples of disc tissues and osteophytes were harvested during the decompression operation. The hematoxylin-eosin staining, Masson trichrome staining and Safranin O-fast green staining were used to compare the histological differences between the two groups. And the distribution and content of transforming growth factor (TGF)-β1, p-Smad2 and p-Smad3 between the two groups were compared by a semi-quantitative immunohistochemistry (IHC) method. Results : For all the disc tissues, the content of disc cells and collagen fibers decreased gradually from the outer annulus fibrosus (OAF) to the central nucleus pulposus (NP). Compared with group NO, the number of disc cells in group O increased significantly. But for proteoglycan in the inner annulus fibrosus (IAF) and NP, the content in group O decreased significantly. IHC analysis showed that TGF-β1, p-Smad2, and p-Smad3 were detected in all tissues. For group O, the content of TGF-β1 in the OAF and NP was significantly higher than that in group NO. For p-Smad2 in IAF and p-Smad3 in OAF, the content in group O were significantly higher than group NO. Conclusion : Histologically, cervical disc degeneration in patients with DO is more severe than that without DO. Local higher content of TGF-β1, p-Smad2, and p-Smad3 are involved in the disc degeneration with DO. Further studies with multi-approach analyses are needed to better understand the role of TGF-β/Smads signaling pathway in the disc degeneration with DO.
The objective of this study was conducted for elucidation of effects of the NO$_3$$^{[-10]}$ -N. NH$_4$$^{+}$-N ratio in the nutrient solution on the growth and quality of welsh onion(Allium fistulosum L.). The pH of nutrient solution increased in NO$_3$$^{[-10]}$ -N : NH$_4$$^{+}$-N ratio of 9 : 1 treatment, decreased in 1 : 1, 1 : 3 treatment, but was stable in 3 : 1 treatment during cultivation. The apparent growth of welsh onion was best in the treatment of 9 : 1(NO$_3$$^{[-10]}$ -N : NH$_4$$^{+}$-N ratio), however the treatment of 1 : 3 resulted in poor growth. The NO$_3$$^{[-10]}$ -N content of the plants increased in proportion to that content of nutrient solution. Pyruvic acid content of welsh onion was highest at 9 : 1, 3 : 1 treatment, and lowest at 1 : 3 treatment.tment.
Journal of The Korean Society of Grassland and Forage Science
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v.16
no.4
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pp.315-326
/
1996
An experiment was carried out to determine effect of cultivar, drying agent concentration and preservative on the moisture content, temperature and quality changes of fall harvested oat(Avena sativa L.) hay during field curing and storage. The main plots consisted of the cultivar such as 'Foothill' and 'Swan', the subplots consisted of drying agent concentration such as 7Skg/ha(C1), lOSkglha(C2) and control(C0) and the sub-subplots consisted of preservative such as buffered acid(BA), lactic bacteria inoculant(LB) and control(C0). Moisture contents of 'Foothill' and 'Swan' oat cultivar at harvest were 635.5 and 385.4%DW(Dry weight), respectively, but the final moisture contents of the oat cultivars were 117 and 86%DW. The moisture content of cut oat was not affeded by drying agent concentration. Core temperature of baled 'Swan' oat hay was a little higher than that of 'Foothill' and no consistent preservative effect was found. Total nitrogen content of 'Swan' oat hay was 3.08% and that of 'Foothill' oat hay was 3.45%(P<0.05). Drying agent treatment increased total nitrogen content of 'Swan'(P <0.05), but preservative had no effect. Crude fiber content of oat cultivar was increased as the curing progressed(P<0.05) and the difference of crude fiber content of oat cultivar was maintained through the whole curing period. Effect of drying agent concentration on crude fiber content was not found after preservation. Crude fiber content of 'Swan' oat hay was higher than that of 'Foothill' by 1.O% in ADF and 3.1 % in NDF(P<0.05). No preservative effect was found in crude fiber content. According to RFV of oat cultivar at harvest, forage quality of 'Foothill' and 'Swan' was Prime and Grade 1, respectively, but that of both cultivm was above Grade 2 at the final curing day. RFV of the stored 'Foothill' and 'Swan' oat hay was 122 and 114(P<0.05), respectively, and the quality of oat hay was classified as Grade 1 and 2, respectively, according to the forage quality standard assigned by AFGC.
Journal of the Korean Society of Food Science and Nutrition
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v.42
no.2
/
pp.153-160
/
2013
The peel from seven types of citrus was extracted with 80% methanol, and their phenolic compound content, oxygen radical absorbance capacity (ORAC), inhibitory activities of nitric oxide (NO), and reactive oxygen species (ROS) production induced by LPS and t-BHP in LPS-activated RAW 264.7 cells were measured. Total phenolic content was high in Yungkyool, Cheonhyehyang, and Jinkyool (30.6, 30.2, and 28.2 mg GAE/g, respectively), while total flavonoid content was high in Yungkyool and Jinkyool (30.3 and 25.5 mg RE/g, respectively). ORAC was the highest at 1,076 mM TE/g in Yungkyool, followed by Cheonhyehyang (1,012), Jinkyool (984), and Hallabong (914). High inhibitory activity against NO production was shown in Cheonhyehyang, Yungkyool, and Jinkyool with $IC_{50}$ values of 215.3, 259.2, and 328.9 ${\mu}g/mL$, respectively. LPS-induced ROS production was inhibited by 16.4% and 12.8% in Hallabong and Jinkyool, while t-BHP-induced ROS production was inhibited by 28.7%, 26.1%, and 26.6% in Jinkyool, Hallabong, and Cheonhyehyang, respectively. Correlation coefficients between total phenolic, total flavonoid content, and ORAC were 0.884 and 0.855. Inhibitory activity against NO production showed higher correlation with total phenolic content than total flavonoid content. It was concluded that citrus peels had potent antioxidant activities and could be used as natural antioxidants in the food and pharmaceutical industries.
In this study, the photo-deposition method was used to introduce Ag onto the surface of TiO2 to synthesize an Ag-TiO2 composite. The effects of the varying amounts of AgNO3 precursor and annealing time periods on the Ag content in the composites, as well as their antibacterial characteristics under visible light conditions were studied. SEM analysis revealed the spherical morphology of the Ag-TiO2 composite. Compared with TiO2, the Ag particles were too small to be observed. An XPS analysis of the Ag-TiO2 surface confirmed the Ag content and showed the peak intensities for elements such as Ag, Ti, O, C, and Si. The highest Ag content was observed when 33.3 wt.% of AgNO3 and an annealing time of 6 h were employed; this was the optimum annealing time for Ti-Ag-O bonding, in that the lowest number of O bonds and the highest number of Ag bonds were confirmed by XPS analysis. Superior antibacterial properties against Bacillus and Escherichia coli, in addition to the widest inhibition zones were exhibited by the Ag-TiO2 composite with an increased Ag content in a disk diffusion test, the bacterial reduction rate against Staphylococcus aureus and Escherichia coli being 99.9%.
Journal of The Korean Society of Grassland and Forage Science
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v.19
no.2
/
pp.141-146
/
1999
This experiment was conducted to investigate the effects of application seasons and the level of cattle slurry on nitrate contents in soils of rye cultivation under climatic conditions of Korea. The main plots were appliaction seasons(100% application in spring, 100% application in autumn, 50% application in spring and autumnn after dividing cattle slurry into two equal parts) and subplots were the levels of cattle slurry-N application(0, 50, 100 and $200kgN\;ha^{-1}\;year^{-1}$). Cattle slurry was spred evenly over the soil surface in spring (end of March) and autumn(early of November). The experiment was done at Gongiam, Kwangju, Kyung-gi for 3 years, from 1994 to 1996. The results obtained are summarized as follows; 1. Nitrate content in soils was not influenced by application seasons of cattle slurry. 2. Nitrate content in soils increased as the level of cattle slurry application increased, and the average nitrate content became 3.6mg per liter. 3. Nitrate content in the soil depth from 0 to 20cm increased as the level of cattle slurry application increased, and the average nitrate content became 28.9mg per liter. However, nitrate content in the soil depth from 40 to 60cm was the lowest(7.5-8.3mg per liter).
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