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http://dx.doi.org/10.3746/jkfn.2013.42.2.153

Phenolic Compound Content and Antioxidant Activity of Citrus Peels  

Hwang, Joon-Ho (Dept. of Biology, Jeju National University)
Park, Kyeong-Yeol (Dept. of Food Bioengineering, Jeju National University)
Oh, You-Sung (Dept. of Biology, Jeju National University)
Lim, Sang-Bin (Biotechnology Regional Innovation Center, Jeju National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.2, 2013 , pp. 153-160 More about this Journal
Abstract
The peel from seven types of citrus was extracted with 80% methanol, and their phenolic compound content, oxygen radical absorbance capacity (ORAC), inhibitory activities of nitric oxide (NO), and reactive oxygen species (ROS) production induced by LPS and t-BHP in LPS-activated RAW 264.7 cells were measured. Total phenolic content was high in Yungkyool, Cheonhyehyang, and Jinkyool (30.6, 30.2, and 28.2 mg GAE/g, respectively), while total flavonoid content was high in Yungkyool and Jinkyool (30.3 and 25.5 mg RE/g, respectively). ORAC was the highest at 1,076 mM TE/g in Yungkyool, followed by Cheonhyehyang (1,012), Jinkyool (984), and Hallabong (914). High inhibitory activity against NO production was shown in Cheonhyehyang, Yungkyool, and Jinkyool with $IC_{50}$ values of 215.3, 259.2, and 328.9 ${\mu}g/mL$, respectively. LPS-induced ROS production was inhibited by 16.4% and 12.8% in Hallabong and Jinkyool, while t-BHP-induced ROS production was inhibited by 28.7%, 26.1%, and 26.6% in Jinkyool, Hallabong, and Cheonhyehyang, respectively. Correlation coefficients between total phenolic, total flavonoid content, and ORAC were 0.884 and 0.855. Inhibitory activity against NO production showed higher correlation with total phenolic content than total flavonoid content. It was concluded that citrus peels had potent antioxidant activities and could be used as natural antioxidants in the food and pharmaceutical industries.
Keywords
citrus peel; phenolic compounds; antioxidant activity; antiinflammatory effect;
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1 Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84.   DOI   ScienceOn
2 Gaunt IF, Feuer G, Fairweather FA, Gilbert D. 1965. Liver response tests. IV. Application to short-term feeding studies with butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Food Cosmet Toxicol 3: 433-443.   DOI   ScienceOn
3 Choi SY, Ko HC, Ko SY, Hwang JH, Park JG, Kang SH, Han SH, Yun SH, Kim SJ. 2007. Correlation between flavonoid content and the NO production inhibitory activity of peel extracts from various citrus fruits. Biol Pharm Bull 30: 772-778.   DOI   ScienceOn
4 Erlund I. 2004. Review of the flavonoids quercetin, hesperetin, and naringenin, dietary sources, bioactivities, bioavailability, and epidemiology. Nutr Res 24: 851-874.   DOI   ScienceOn
5 Kim YD, Mahinda S, Koh KS, Jeon YJ, Kim SH. 2009. Reactive oxygen species scavenging activity of Jeju native citrus peel during maturation. J Korean Soc Food Sci Nutr 38: 462-469.   과학기술학회마을   DOI   ScienceOn
6 Kim YD, Ko WJ, Koh KS, Jeon YJ, Kim SH. 2009. Composition of flavonoids and antioxidative activity from juice of Jeju native citrus fruits during maturation. Korean J Nutr 42: 278-290.   과학기술학회마을   DOI   ScienceOn
7 Ahn MS, Kim HJ, Seo MS. 2007. A study on the antioxidative and antimicrobial activities of the Citrus unshiu peel extracts. Korean J Food Culture 22: 454-461.
8 Zhang Q, Zhang J, Shen J, Silva A, Dennis DA, Barrow CJ. 2006. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. J Appl Phycol 18: 445-450.   DOI   ScienceOn
9 Chang CC, Yang MH, Wen HM, Chern JC. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10: 178-182.
10 Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL. 2002. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 50: 4437-4444.   DOI   ScienceOn
11 Ou B, Hampsch-Woodill M, Prior RL. 2001. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem 49: 4619-4626.   DOI   ScienceOn
12 Amano F, Noda T. 1995. Improved detection of nitric oxide radical (NO․) production in an activated macrophage culture with a radical scavenger, carboxy PTIO and Griess reagent. FEBS Lett 368: 425-428.   DOI   ScienceOn
13 Kimura K, Ito S, Nagino M, Isobe K. 2008. Inhibition of reactive oxygen species down-regulates protein synthesis in RAW 264.7. Biochem Biophys Res Commun 372: 272-   DOI   ScienceOn
14 Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. 2001. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 74: 418-425.
15 Park GH, Lee SH, Kim HY, Jeong HS, Kim EY, Yun YW, Nam SY, Lee BJ. 2011. Comparison in antioxidant effects of four citrus fruits. J Fd Hyg Safety 26: 355-360.   과학기술학회마을
16 Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. 2009. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci 22: 277-281.
17 Benavente-Garcia O, Castillo J. 2008. Update on uses and properties of citrus flavonoids: new findings in anticancer, cardiovascular, and anti-inflammatory activity. J Agric Food Chem 56: 6185-6205.   DOI   ScienceOn
18 Cai Y, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional chinese medicinal plants associated with anticancer. Life Sci 74: 2157- 2184.   DOI   ScienceOn
19 Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53: 4290-4302.   DOI   ScienceOn
20 Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R. 2003. Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity) of plasma and other biological and fod samples. J Agric Food Chem 51: 3273-3279.   DOI   ScienceOn
21 Kim GN, Shin JG, Jang HD. 2009. Antioxidant and antidiabetic activity of Dangyuja (Citrus grandis Osbeck) extract treated with Aspergillus saitoi. Food Chem 117: 35-41   DOI   ScienceOn
22 Khan MK, Abert-Vian M, Fabiano-Tixier A, Dangles O, Chemat F. 2010. Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chem 119: 851-858.   DOI   ScienceOn
23 Nathan C. 1992. Nitric oxide as a secretory product of mammalian cells. FASEB J 6: 3051-3064.
24 McDaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. 1996. Cytokines and nitric oxide in islet inflammation and diabetes. Proc Soc Exp Biol Med 211: 24-32.   DOI
25 Carter WO, Narayanan PK, Robinson JP. 1994. Intracellular hydrogen peroxide and superoxide anion detection in endothelial cells. J Leukoc Biol 55: 253-258.
26 Radi R, Beckman JS, Bush KM, Freeman BA. 1991. Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J Biol Chem 266: 4244-4250.
27 Uchikura K, Wada T, Hoshino S, Nagakawa Y, Aiko T, Bulkley GB, Klein AS, Sun Z. 2004. Lipopolysaccharides induced increases in fas ligand expression by kupffer cells via mechanisms dependent on reactive oxygen species. American J Physiol: Gastrointest Liver Physiol 287: 620-626   DOI   ScienceOn
28 Kennedy CH, Church DF, Winston GW, Pryor WA. 1992. Tert-butyl hydroperoxide-induced radical production in rat liver mitochondria. Free Radical Biol Med 12: 381-387.   DOI   ScienceOn
29 Mario M, Sonia R, Raquel M, Laura B, Lusi G. 2005. Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in human hepatoma cell line (HepG2). J Biochem Mol Toxicol 19: 119-128.   DOI   ScienceOn
30 Bass DA, Parce JW, Dechatelet LR, Szejda P, Seeds MC, Thomas M. 1983. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J Immunol 130: 1910-1917.
31 Ciz M, Pavelkova M, Gallova L, Kralova J, Kubala L, Lojek A. 2008. The influence of wine polyphenols on reactive oxygen and nitrogen species production by murine macrophages RAW 264.7. Physiol Res 57: 393-402.
32 Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. 2004. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52: 794-804.
33 Forman HJ, Dorio RJ, Skelton DC. 1987. Hydroperoxide-induced damage to alveolar macrophage function and membrane integrity: alterations in intracellular-free $Ca^{2+}$ and membrane potential. Arch Biochem Biophys 259: 457-465.   DOI   ScienceOn
34 Kabe Y, Ando K, Hirao S, Yoshida M, Handa H. 2005. Redox regulation of NF-${\kappa}B$ activation: distinct redox regulation between the cytoplasm and the nucleus. Antioxid Redox Signal 7: 395-403.   DOI   ScienceOn
35 Pattanaik U, Prasad K. 1996. Endotoxemia and oxidative stress. Ann NY Acad Sci 793: 506-510.   DOI