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http://dx.doi.org/10.5338/KJEA.2005.24.3.245

The Effect of Mixed Amino Acids on Nitrate Uptake and Nitrate Assimilation in Leafy Radish  

Liu, Xing-Quan (Department of Agricultural Chemistry, Chungnam National University)
Kim, Young-Sun (Bio NEL.CO., LTD.)
Lee, Kyu-Seung (Department of Agricultural Chemistry, Chungnam National University)
Publication Information
Korean Journal of Environmental Agriculture / v.24, no.3, 2005 , pp. 245-252 More about this Journal
Abstract
The objective of the present work was to determine the corresponding uptake and assimilation of ${NO_3}^-$ in roots and shoots of leafy radish by applying of mixed amino acids (MAA). The amino acids used in this experiment were alanine (Ala), ${\beta}-alanine\;({\beta}-Ala)$, aspartic acid (Asp), asparagines (Asn), glutamic acid (Glu), glutamine (Gln), and glycine (Gly). Leafy radish was grown by conventional fertilization with macro- and micronutrients under controlled conditions. The 15-day-old seedlings were treated 0, 0.3 and 3.0 mM of MAA containing 5 mM ${NO_3}^-$ in growth medium. Nitrate uptake was determined by following ${NO_3}^-$ depletion from the uptake solution. The activity of the enzymes related to the process of ${NO_3}^-$ reduction (NR: nitrate reductase; NiR: nitrite reductase; GS: glutamine synthetase) and the content of ${NO_2}^-\;and\;{ND_3}^-$ were analyzed in shoots and roots. The results of this study showed that ${NO_3}^-$ uptake was inhibited 38% with treatment of 0.3 mM of MAA. However, there was more than three times increase of N03- uptake in 3.0 mM MAA. In addition, the enzymatic activities were positively affected by the high MAA rate. Finally, the ${NO_3}^-$ content was increased slightly both in shoots and roots of leafy radish by MAA treatments.
Keywords
leafy radish; nitrate uptake; amino acid; nitrate assimilation;
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