Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Histological Changes of Cervical Disc Tissue in Patients with Degenerative Ossification

  • Xiong, Yang (School of Materials Science and Engineering, Tsinghua University) ;
  • Yang, Ying-Li (Department of Traditional Chinese Medicine, Peking Union Medical College Hospital) ;
  • Gao, Yu-Shan (School of Chinese Medicine, Beijing University of Chinese Medicine) ;
  • Wang, Xiu-Mei (School of Materials Science and Engineering, Tsinghua University) ;
  • Yu, Xing (Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine)
  • Received : 2021.04.08
  • Accepted : 2021.06.20
  • Published : 2022.03.01
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Abstract

Objective : To explore the histological feature of the cervical disc degeneration in patients with degenerative ossification (DO) and its potential mechanisms. Methods : A total of 96 surgical segments, from cervical disc degenerative disease patients with surgical treatment, were divided into ossification group (group O, n=46) and non-ossification group (group NO, n=50) based on preoperative radiological exams. Samples of disc tissues and osteophytes were harvested during the decompression operation. The hematoxylin-eosin staining, Masson trichrome staining and Safranin O-fast green staining were used to compare the histological differences between the two groups. And the distribution and content of transforming growth factor (TGF)-β1, p-Smad2 and p-Smad3 between the two groups were compared by a semi-quantitative immunohistochemistry (IHC) method. Results : For all the disc tissues, the content of disc cells and collagen fibers decreased gradually from the outer annulus fibrosus (OAF) to the central nucleus pulposus (NP). Compared with group NO, the number of disc cells in group O increased significantly. But for proteoglycan in the inner annulus fibrosus (IAF) and NP, the content in group O decreased significantly. IHC analysis showed that TGF-β1, p-Smad2, and p-Smad3 were detected in all tissues. For group O, the content of TGF-β1 in the OAF and NP was significantly higher than that in group NO. For p-Smad2 in IAF and p-Smad3 in OAF, the content in group O were significantly higher than group NO. Conclusion : Histologically, cervical disc degeneration in patients with DO is more severe than that without DO. Local higher content of TGF-β1, p-Smad2, and p-Smad3 are involved in the disc degeneration with DO. Further studies with multi-approach analyses are needed to better understand the role of TGF-β/Smads signaling pathway in the disc degeneration with DO.

Keywords

Acknowledgement

This study was supported by the National Key R&D Program of China (2020YFC1107600) and Shandong Province Key R&D Program of China (2019JZZY011106).

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