• Title/Summary/Keyword: NIH/3T3

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N-methyl-N'-nitro-N-nitrosoguanidine Reduces the Intracellular Calcium Level Through NAD Depletion in NIH3T3 Cells

  • Yoon, Yoo-Sik;Shin, In-Cheol;Kim, Jin-Woo;Kang, Ke-Won;Joe, Cheol-O
    • BMB Reports
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    • v.28 no.5
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    • pp.392-397
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    • 1995
  • The effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on the intracellular $Ca^{2+}$ level was studied in NIH3T3 fibroblast cells. A reduction of the intracellular $Ca^{2+}$ level was observed after exposure to 300 ${\mu}m$ MNNG. However, the intracellular level of $IP_3$, a well-known regulator of $Ca^{2+}$ release from internal storage, was not changed by MNNG treatment. Instead, a reduction of the intracellular NAD level was observed. NAD as well as $IP_3$ stimulated intracellular $Ca^{2+}$ release from permeabilized cells. The treatment of 3-aminobenzamide, which inhibited the MNNG-induced reduction of the NAD level, also prevented the MNNG-induced decrease of the $Ca^{2+}$ level. Our data suggest that MNNG reduces the intracellular $Ca^{2+}$ level by NAD depletion in NIH3T3 cells.

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Induction of MAP kinase phosphatase 3 through Erk/MAP kinase activation in three oncogenic Ras (H-, K- and N-Ras)-expressing NIH/3T3 mouse embryonic fibroblast cell lines

  • Koo, JaeHyung;Wang, Sen;Kang, NaNa;Hur, Sun Jin;Bahk, Young Yil
    • BMB Reports
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    • v.49 no.7
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    • pp.370-375
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    • 2016
  • Ras oncoproteins are small molecular weight GTPases known for their involvement in oncogenesis, which operate in a complex signaling network with multiple effectors. Approximately 25% of human tumors possess mutations in a member of this family. The Raf1/MEK/Erk1/2 pathway is one of the most intensively studied signaling mechanisms. Different levels of regulation account for the inactivation of MAP kinases by MAPK phosphatases in a cell type- and stimuli-dependent manner. In the present study, using three inducible Ras-expressing NIH/3T3 cell lines, we demonstrated that MKP3 upregulation requires the activation of the Erk1/2 pathway, which correlates with the shutdown of this pathway. We also demonstrated, by applying pharmacological inhibitors and effector mutants of Ras, that induction of MKP3 at the protein level is positively regulated by the oncogenic Ras/Raf/MEK/Erk1/2 signaling pathway.

Regenerative Effects of Houttuynia cordata Extracts against Cadmium-induced Cytotoxicity in NIH 3T3 fibroblasts Cultures (VII) (카드뮴 독성으로 손상된 NIH 3T3 섬유모세포에 대한 어성초 추출물의 재생효과(VII))

  • Lee Jeong-Ho;Oh Hyun-Ju;Jeong Seung-Il;Chun Hyun-Ja;Han Do-Seok;Lee Hyun-Ok;Ahn Jong-Woong;Lee Mee-Hee;Baek Seung-Hwa
    • Journal of Society of Preventive Korean Medicine
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    • v.5 no.2
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    • pp.122-128
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    • 2001
  • This study was carried out to evaluate antitoxic effects of Houttuynia cordata extract on cadmium by colorimetric methods. The antitoxic activity ofc in NIH 3T3 fibroblasts was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells. The concentration of 10-2 mg/ml of Houttuynia cordata extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were antitoxic and tend to regenerate. These results suggest that the ethyl acetate extract of Houttuynia cordata retains a potential antitoxic activity.

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Decrease of Genotoxicity by Red Ginseng Root Extract (I) - Decrease of UV -induced Genotoxicity by Red Ginseng Root Extract in Cultured NIH3T3 Cells (홍삼 추출물에 의한 유전독성 감소효과 (I) - 배양 NIH3T3 세포에서 자외선에 의한 유전독성의 감소에 미치는 홍삼추출물 처리효과)

  • 김완주;유병수
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.24 no.1
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    • pp.74-86
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    • 1998
  • We have studied the effects of red ginseng root extract on the decrease of UV-induced genotoxicity in cultured NIH3T3 cells. The increase in survival and the recovery from DNA synthesis inhibition in UV-irradiated cells as a function of normal medium incubation time was potentiated by the presence of the ginseng extract. The extract also increased the UV-induced excision repair as determined by unscheduled DNA synthesis. The amount of UV-induced DNA single strand breaks that are accumulated by polymerase inhibitors was significantly increased by the presence of the extract. These results suggest that the red ginseng extract activates the incision/excision step of UV-induced repair and could be used as a reagent for protecting UV-induced genotoxicity and cytotoxicity.

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Decrease of Genotoxicity by Red Ginseng Root Extract (II) -Decrease of MMS- induced Genotoxicity by Red Ginseng Root Extract in Cul tared NIH3T3 Cells (홍삼 추출물에 의한 유전독성 감소효과(II) -배양 NIH3T3 세포에서 MMS에 의한 유전독성의 감소에 미치는 홍삼추출물 처리효과)

  • 차재영;유병수
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.24 no.1
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    • pp.87-99
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    • 1998
  • We have studied the effects of red ginseng root extract on the derease of MMS-induced gemotoxicity in cultured NIH3T3 cells. The increase in survival and the recovery from DNA synthesis inhibition in MMS-treated cells as a function of normal medium incubation time was potentiated, at a rate higher than those in UV-irradiated cells, by the presence of the ginseng extract. The extract also increased the MMS-induced excision repair as determined by unscheduled DNA synthesis. The amount of MMS-induced DNA single strand breads that are accumulated by polymerase inhibitors was increased, but as a rate lower rate than in UV-induced strand break, by the presence of the extract. These results suggest that the red ginseng extract increase MMS-induced repair and could be used as a reagent for protectiong alkylating agent-induced genotoxicity and cytotoxicity.

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Cytoprotective Effect of Galgeuntang Extract on Cadmium-Induced Cytotoxicity (갈근탕 추출물의 카드뮴 독성에 대한 세포 보호효과)

  • Park, So-Yun;Baek, Jong-Min;Baek, Seung-Hwa
    • YAKHAK HOEJI
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    • v.54 no.3
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    • pp.151-156
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    • 2010
  • Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), $MTT_{50}$ group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and $IC_{50}$ was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

Fabrication of PDMS Stencil using Gas Blowing for Micropatterned 3T3 Cell Culture (가스 블로잉을 이용한 PDMS 스텐실 제작 및 3T3 세포의 마이크로 패터닝)

  • Choi, Jin Ho;Kim, Gyu Man
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.2
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    • pp.236-240
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    • 2013
  • In this presentation, we propose a fabrication method of PDMS stencil to apply into a localized culture of NIH/3T3 cells. PDMS stencil was fabricated by nitrogen gas blowing and soft lithography from preparing SU-8 master mold by photolithography. PDMS stencil pattern was production of the circle size 20 to $500{\mu}m$. In the culture test of PDMS stencil, a stencil was placed on a glass disk. The NIH/3T3 cells were successfully cultured into micropatterns by using the PDMS stencil. The results showed that cells could be cultured into micropatterns with precisely controlled manner at any shapes and specific size for bioscience study and bioengineering applications.

Anticancer Effect of Ferulic Acid on Cultured Human Skin Melanoma Cells

  • Son, Byoung-Kwan;Choi, Yu-Sun;Sohn, Young-Woo
    • Biomedical Science Letters
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    • v.12 no.4
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    • pp.457-461
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    • 2006
  • It is demonstrated that phenolic compound has cytotoxic effect on cancer cells. Recently, ferulic acid is involved in anticancer activity by showing the decrease of cell viability in cancer cells. But, the anticancer mechanism of ferulic acid is left unknown. The purpose of this study was to examine the anticancer activity of ferulic acid on NIH3T3 fibroblasts and human skin melanoma cells (SK-MEL-3). The anticancer activity was measured by determining the cytotoxicy of ferulic acid on these cells. The cytotoxicity was measured by cell viability via XTT assay in these cells. In this study, ferulic acid decreased cell viability according to the dose-dependent manners after human skin melanoma cells were treated with various concentrations of ferulic acid for 48 hours. especially, ferulic acid remarkably decreased cell viability at a concentration of $120{\mu}M$ compared with control in human skin melanoma cells. While, ferulic acid did not show the significant decrease of cell viability at concentrations of $30{\sim}120{\mu}M$ in NIH3T3 fibroblasts. These results suggest that ferulic acid showed anticancer activity in cancer cells such as human skin melanoma cells by the decrease of cell viability significantly.

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Anticancer and Antioxidant Effects of Saturated Fatty Acid against Skin Cancer Cell Lines (포화지방산의 피부암 세포주에 대한 항암 및 항산화 효과)

  • Han, Du-Seok;Park, Yoon-Kyu;Kim, Hyun-Jin;Lee, Jae-Suk;Baek, Seung-Hwa
    • Journal of Society of Preventive Korean Medicine
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    • v.12 no.3
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    • pp.47-58
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    • 2008
  • The anticancer and antioxidant effect of different lengths of saturated fatty acids was tested on NIH3T3 fibroblasts and human skin melanoma cellsn in this study. The cell existence rate and antioxidizing capacity and optic reservation of cells were observed. This saturated fatty acid was concentration-dependent. IC50 Concentrations in NIH3T3 fibroblasts, human skin melanoma cells and DPPH radical scavenging activity of fatty acid was increasing the order of carbochain length ; caprylic acid < lauric acid < palmitic acid < stearic acid. The reduction in cell number and morphological change in human skin melanoma cells was increasing the order of carbochain length ; caprylic acid < lauric acid < palmitic acid < stearic acid. These results suggest that carbochain length of fatty acid can be used as structure-activity relationships for anticancer and antioxidant.

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Non-histone protein HMGB1 inhibits the repair of damaged DNA by cisplatin in NIH-3T3 murine fibroblasts

  • Yusein-Myashkova, Shazie;Ugrinova, Iva;Pasheva, Evdokia
    • BMB Reports
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    • v.49 no.2
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    • pp.99-104
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    • 2016
  • The nuclear non-histone protein high mobility group box (HMGB) 1 is known to having an inhibitory effect on the repair of DNA damaged by the antitumor drug cisplatin in vitro. To investigate the role of HMGB1 in living cells, we studied the DNA repair of cisplatin damages in mouse fibroblast cell line, NIH-3T3. We evaluated the effect of the post-synthetic acetylation and C-terminal domain of the protein by overexpression of the parental and mutant GFP fused forms of HMGB1. The results revealed that HMGB1 had also an inhibitory effect on the repair of cisplatin damaged DNA in vivo. The silencing of HMGB1 in NIH-3T3 cells increased the cellular DNA repair potential. The increased levels of repair synthesis could be "rescued" and returned to less than normal levels if the knockdown cells were transfected with plasmids encoding HMGB1 and HMGB1 K2A. In this case, the truncated form of HMGB1 also exhibited a slight inhibitory effect.