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Antioxidative and Biological Activites of Extracts of Sweetpotato Tips (고구마 끝순 추출물의 항산화 및 생리활성)

  • Lee, Joon-Seol;Park, Yang-Kyun;Ahn, Young-Sup;Kim, Hag-Sin;Chung, Mi-Nam;Jeong, Byeong-Choon;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.2
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    • pp.228-238
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    • 2007
  • This study was conducted to increase sweetpotato utilization and to determine the vegetative value of sweetpotato tips by investigating the phenolic compounds, antioxidative effect in oil, electron donating ability, nitrite scavenging effect and ACE inhibition activities. The phenolic compounds present in sweetpotato tips are the gallic, chlorogenic, gentisic, caffeic, couramic and ferulic acid, which are 16-122 times higher compared to other vegetables such as spinach, soybean sprout, and perilla leaves. In each solvent extract, the total phenolic compounds (175.8mg/g) was composed of 55% EtOAc extraction and 39% BuOH extract, respectively. The results of induction period using the Rancimat method showed that the antioxidant activity of SP tips was higher than the tocopherol or BHT. The relative levels of each solvent extract in SP tips were as follows: EtOAc>BHT>BuOH>Tocopherol>Water>$CHCl_3$>Hexane. The peroxide value was measured every 5 days for 25 days during storage and results showed that the peroxide value, the tips, tuberous root and tocopherol were lower compared to spinach, soybean sprout and perilla leaves. Nitrite scavenging effects were excellent in sweetpotato tips, perilla leaves and soybean sprout, especially, inhibition rate of perilla leaves (72%) were superior to the others. In process of solvent extraction, activity of BuOH and water extractions were the best. ACE inhibition activity in sweetpotato tips was 1.5 times higher than in tuberous roots and $1.9{\sim}3.7$ times higher than in spinach, soybean sprout, perilla leaves.

Variation of Protein, Oil, Fatty Acid, and Sugar Contents in Black Soybean Cultivars According to Different Latitudes (재배지 위도차에 따른 검정콩의 조단백, 조지방, 지방산 및 유리당의 함량 변이)

  • Hong, Seung-Beom;Lee, Su-Jin;Kim, Young-Hak;Hwang, Young-Sun;Yoon, Kwang-Hee;Lee, Sung-In;Nam, Mi-Young;Song, Lee-Seul;Baek, In-Youl;Kim, Hyeun-Kyeung;Choung, Myoung-Gun
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.120-128
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    • 2010
  • This experiment was conducted to investigate the variation of crude protein, crude oil, fatty acid, and sugar contents in five Korean domestic black soybean cultivars grown at different latitudinal locations, a high latitude, Suwon ($37^{\circ}$16'N) and a low latitude, Milyang ($35^{\circ}$30'N). The crude protein content was highest in Geomjeongkong # 3 (43.9%) and crude oil content was highest in Geomjeongkong # 4 (21.8%) among the five cultivars. Crude protein and oil contents in black soybean cultivars except Geomjeongkong # 4 were not significantly different between high latitude and low latitude. In most black soybean cultivars grown at high latitude, oleic acid content was higher, while linoleic acid, and linolenic acid contents were lower compared to low latitude. Sucrose content in Geomjeongkong # 3, raffinose contents in Geomjeongkong # 3, # 4, Ilpumgeomjeongkong, and Cheongjakong, and stachyose content in Geomjeongkong # 1 grown at low latitude was higher compared to high latitude. The variations of crude protein, crude oil and fatty acid contents seemed to be affected by genotype than growing locations according to different latitude as they did not show the significant interaction between cultivars and locations. In contrast, the variations of glucose, sucrose, and stachyose contents maybe affected by environmental condition as different latitude than the genotype because they showed the significant interaction between cultivars and locations.

Effect of Cyantraniliprole against of Bemisia tabaci and Prevention of Tomato Yellow Leaf Curl Virus (TYLCV) (Cyantraniliprole의 담배가루이에 대한 살충활성과 이를 통한 토마토황화잎말림바이러스 예방)

  • Lee, Mun-Haeng;Lee, Hee-Kyoung;Lee, Hwan-Gu;Lee, Sun-Gye;Kim, Jeom-Soon;Kim, Sung-Eun;Kim, Young-Shik;Suh, Jeung-Keun;Youn, Young-Nam
    • The Korean Journal of Pesticide Science
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    • v.18 no.1
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    • pp.33-40
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    • 2014
  • To control Bemisia tabaci on tomato, we applied five different combinations of chemical treatments as below: 1) treatment of combinations of cyantraniliprole on the root area and leaf with the existing registered chemicals three times; 2) treatment of combinations of cyantraniliprole on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 3) treatment of combinations of dinotefuran on the root area and cyantraniliprole on the leaf with the existing registered chemicals three times; 4) treatment of combinations of dinotefuran on the root area and dinotefuran + emamectin benzoate on the leaf with the existing registered chemicals three times; 5) untreated control plot (Table 1). Twenty days after treatment ($17^{th}$ Aug.), the number of population of B. tabaci was zero on the 1, 2, 3, 4 treatments of combinations, and only 2 individuals were found on the 5 treatment of combination per each 20 plant. On $17^{th}$ Sep., in the last observation, the average number of population of B. tabaci was 10.3, 10.3, 10.6 on the 1, 2, 3 treatments of combinations on the 20 plants per each combination, however, the average number of 23.3 and 37.6 were examined on the 4 and 5 treatments of combinations, respectively. TYLCV was not occurring on the 1 and 2 treatments of combinations, and presented only 3% and 17% on the 3 and 4 treatments of combinations, respectively, which indicates that the treatments (1-4) should be effective on TYLCV control as considering that 33% of TYLCV occurred on the untreated control plot. However, after the third flowering period, there is no difference among the five combinations. The amount of products was 9,148g and 9,698g on the 1 and 2 treatments of combinations, respectively, which was the most among the 5 combinations. The number of fallen fruits and the average weight of fruits showed the similar tendency.

Effects of Green Manure and Carbonized Rice Husk on Soil Properties and Rice Growth (녹비작물 혼파 이용 벼 재배 시 왕겨숯 처리가 벼 생육 및 토양 특성에 미치는 영향)

  • Jeon, Weon-Tai;Seong, Ki-Yeong;Lee, Jong-Ki;Oh, In-Seok;Lee, Young-Han;Ok, Yong-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.484-489
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    • 2010
  • The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. However, the incorporation of green manure crops may be of concern because it can lead to strongly reducing conditions in the submerged soil. This study was conducted to evaluate the effects of rice husk carbon on rice (Oryza sativa L.) cultivation using green manure mixtures (hairy vetch + rye) in rice paddy. Field experiments were conducted in rice paddy soil (Shinheung series, fine loamy, mixed, nonacid, mesic family of Aeric Fluventic Haplaquepts) at the National Institute of Crop Science (NICS), Korea from October 2007 to October 2008. The experiments consisted of three treatments: application or no application of carbonized rice husk, and conventional fertilization. These treatments were subdivided into whole incorporation and aboveground removal of green manure mixtures. The redox potential (Eh) was higher upon application of the carbonized rice husk when compared to no application at 8 and 37 days after transplanting (DAT). The ammonium-N ($NH_4$-N) in soil was highest upon the application of carbonized rice husk + whole green manure incorporation at 17 and 49 DAT. Plant height and tiller number of rice were similar to the $NH_4$-N concentration in soil. Rice yields of application and no application of carbonized rice husk treatment were not significant. However, application of carbonized rice husk improved the soil physical properties such as bulk density and porosity after rice harvest. Therefore, the results of this study suggest that carbonized rice husk could be used as soil amendment for environmentally-friendly rice production under a green manure mixture-rice cropping system.

Monitoring for the Resistance of Botrytis cinerea Causing Gingseng Gray Mold to Procymidone and Its Multiple resistance with the Mixture of Carbendazim/Diethofencarb (인삼 잿빛곰팡이병균의 procymidone에 대한 감수성 변화와 carbendazim/diethofencarb 합제와의 다중 저항성)

  • Lee, Seon-Wook;Kim, Joo-Hyung;Min, Ji-Young;Bae, Young-Seok;Kim, Heung-Tae
    • Research in Plant Disease
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    • v.13 no.3
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    • pp.170-176
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    • 2007
  • Effects of fungicides on the mycelial growth of Botrytis cinerea isolated from ginseng leaves were investigated by an agar dilution method. By using a agar dilution method, it was investigated the effect of fungicides, procymidone, carbendazim and the mixture with both of carbendazim and diethofencarb, on the mycelial growth of Botrytis cinerea isolates, which were isolated from infected leaves of ginseng in 2005 and 2006. With MIC (minimum inhibiton concentration) of procymidone against B. cinerea, pathogens were divided into two groups. While one showed the low MIC between 0.8 and $4.0{\mu}g/ml$, the other showed higher MIC above $20{\mu}g/ml$. In terms of the inhibition ratio of mycelial growth at the indicated concentration of procymidone, isolates of B. cinerea were divided into three groups; the sensitive, the intermediate resistant, and the resistant group. Each group was differentiated by $EC_{50}$; the sensitive group showed below $2.0{\mu}g/ml$, the intermediate resistant group between 2.0 to $5.0{\mu}g/ml$, and resistant group above $5.0{\mu}g/ml$. Compared with the ratio of resistant isolates of B. cinerea in 2005, the ratio in 2006 increased from 19.3% to 27.5%. Furthermore, the average $EC_{50}$ value of them increased from $10.0{\mu}g/ml$ in 2005 to $237.3{\mu}g/ml$ in 2006. The ratio of isolates showing the multiple resistance between procymidone and carbendazim was 40.2%, whereas the ratio was 4.0% showing the multiple resistance in the mixture.

Evaluating efficiency of automatic surface irrigation for soybean production

  • Jung, Ki-yuol;Lee, Sang-hun;Chun, Hyen-chung;Choi, Young-dae;Kang, Hang-won
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.252-252
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    • 2017
  • Nowadays water shortage is becoming one of the biggest problems in the Korea. Many different methods are developed for conservation of water. Soil water management has become the most indispensable factor for augmenting the crop productivity especially on soybean (Glycine max L.) because of their high susceptibility to both water stress and water logging at various growth stages. The farmers have been using irrigation techniques through manual control which farmers irrigate lands at regular intervals. Automatic irrigation systems are convenient, especially for those who need to travel. If automatic irrigation systems are installed and programmed properly, they can even save you money and help in water conservation. Automatic irrigation systems can be programmed to provide automatic irrigation to the plants which helps in saving money and water and to discharge more precise amounts of water in a targeted area, which promotes water conservation. The objective of this study was to determine the possible effect of automatic irrigation systems based on soil moisture on soybean growth. This experiment was conducted on an upland field with sandy loam soils in Department of Southern Area Crop, NICS, RDA. The study had three different irrigation methods; sprinkle irrigation (SI), surface drip irrigation (SDI) and fountain irrigation (FI). SI was installed at spacing of $7{\times}7m$ and $1.8m^3/hr$ as square for per irrigation plot, a lateral pipe of SDI was laid down to 1.2 m row spacing with $2.3L\;h^{-1}$ discharge rate, the distance between laterals was 20 cm spacing between drippers and FI was laid down in 3m interval as square for per irrigation plot. Soybean (Daewon) cultivar was sown in the June $20^{th}$, 2016, planted in 2 rows of apart in 1.2 m wide rows and distance between hills was 20 cm. All agronomic practices were done as the recommended cultivation. This automatic irrigation system had valves to turn irrigation on/off easily by automated controller, solenoids and moisture sensor which were set the reference level as available soil moisture levels of 30% at 10cm depth. The efficiency of applied irrigation was obtained by dividing the total water stored in the effective root zone to the applied irrigation water. Results showed that seasonal applied irrigation water amounts were $60.4ton\;10a^{-1}$ (SI), $47.3ton\;10a^{-1}$ (SDI) and $92.6 ton\;10a^{-1}$ (FI), respectively. The most significant advantage of SDI system was that water was supplied near the root zone of plants drip by drip. This system saved a large quantity of water by 27.5% and 95.6% compared to SI, FI system. The average soybean yield was significantly affected by different irrigation methods. The soybean yield by different irrigation methods were $309.7kg\;10a^{-1}$ from SDI $282.2kg\;10a^{-1}$ from SI, $289.4kg\;10a^{-1}$ from FI, and $206.3kg\;10a^{-1}$ from control, respectively. SDI resulted in increase of soybean yield by 50.1%, 7.0% 9.8% compared to non-irrigation (control), FI and SI, respectively. Therefore, the automatic irrigation system supplied water only when the soil moisture in the soil went below the reference. Due to the direct transfer of water to the roots water conservation took place and also helped to maintain the moisture to soil ratio at the root zone constant. Thus the system is efficient and compatible to changing environment. The automatic irrigation system provides with several benefits and can operate with less manpower. In conclusion, improving automatic irrigation system can contribute greatly to reducing production costs of crops and making the industry more competitive and sustainable.

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Effect of Pre-Germination by Treatment of Soaking on Germination of Soybean (콩의 발아 전 침종처리가 발아에 미치는 영향)

  • Cho, Seong-Woo;Kim, Tae-Sun;Kwon, Soo-Jeong;Roy, Swapan Kumar;Lee, Chul-Won;Kim, Hong-Sig;Woo, Sun-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.1
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    • pp.123-137
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    • 2015
  • This study was carried out to investigate the effect of pre-germination soaking on germination in 90 Korean soybean varieties and identification of protein in seeds of 7 soybean varieties. The results obtained that germination rate of soybean seeds was decreased as amount of soaking water and soaking duration in number of days. Difference in germination rate of soybean seeds was significant at three days soaking with water volume of 90 ml. Water absorption of seeds was rapidly increased during the first 6 hours, followed by slow increase until 24 hours and then decreased 24 to 48 hours after soaking soybean varieties for bean sprout soaked the lowest amount of water, while soybean varieties for cooking with rice showed the lowest seed water content. Dissolved oxygen (DO) in soaking water was rapidly decreased during the first 3 hours after soaking, and then slowly decreased. Soybean varieties for vegetable and early maturity showed the lowest DO during early soaking periods, but showed higher DO after 24 hours than other groups of soybean varieties. Electrical conductivity and Total Dissolved Solid (TDS) were increased as number of soaking days increased. Soybean varieties for vegetable and early maturity showed the highest electrical conductivity and TDS, followed by those for sauce and paste or cooking with rice, while showed the lowest electrical conductivity and TDS, varieties for bean sprout. Among 90 Korean soybean varieties, varieties which showed the highest germination rate were Jangsu-kong for sauce and paste, Sobaegnamul-kong for bean sprout, Seonheuk-kong for cooking with rice, Seunnokkong for vegetable and early maturity. On the ather hand varieties which showed the lowest germination rate were Iksan and Songhak-kong for sauce and paste, Pangsa-kong for bean sprout, Jinyeul-kong for cooking with rice, Sinlok-kong for vegetable and early maturity. Germination rates of soybean seeds were higher when electrical conductivity, TDS and water absorption of seeds were lower. There were negative correlations between electrical conductivity, TDS and water absorption of seeds and germination rate, while there were positive correlations among electrical conductivity, TDS and soybean seed weight.

Effect of Carbon Dioxide Concentration, Temperature, and Relative Drought on Growth Responses and Yield in Spring Potato (Solanum tuberosum L.) (이산화탄소와 온도 그리고 한발 영향에 따른 감자의 생육과 수량반응)

  • Lee, Yun-Ho;Cho, Hyeoun-Suk;Kim, Jun-Hwan;Sang, Wan-Gyu;Shin, Pyong;Baek, Jae-Kyeong;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.149-158
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    • 2018
  • Agriculture is strongly influenced by climate change such as increased temperature and carbon dioxide ($CO_2$). This study describes the effects of climate change elevated $CO_2$, temperature, and relative drought on growth responses and yield in potato (Solanum tuberosum L.). The assessment was conducted for spring seasons in Soil-Plant-Atmosphere Research (SPAR) chamber at National Institute of Crop Science (NICS). Potatoes exhibit a positive response to $CO_2$ enrichment but water stress primarily reduces potato canopy and tuber yield. Elevated $CO_2$ and temperature increased both dry weight and tuber yield. Elevated $CO_2$ and temperature influenced SPAR 2 plants to a larger, and tuber increased yield up to 28% of than in SPAR 1(30-year average temperature at 450 ppm of $CO_2$). Our study findings indicate that tuber yield increase in potato under high $CO_2$ concentration was due to an increase in the size of individual tubers rather than in the number of the tubers per plant. On other hand, SPAR 3(30-year average temperature $+2.8^{\circ}C$ at 700 ppm of $CO_2$ under water stress) was lower than SPAR 2(30-year average temperature $+2.8^{\circ}C$ at 700 ppm of $CO_2$) nearly 56% of tuber yield due to drought. The results confirm potato drought sensitivity in terms of yield response. The experiment also showed that, in the conditions of climate change, climate change scenarios that improve cropping systems with potato.

Antibacterial and Antimutagenic Effects of Sweetpotato Tips Extract (고구마 끝순 추출물의 항균 및 항돌연변이 효과)

  • Lee, Joon-Seol;Shin, Mee-Jin;Park, Yang-Kyun;Ahn, Young-Sup;Chung, Mi-Nam;Kim, Hag-Sin;Kim, Jeong-Mok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.3
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    • pp.303-310
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    • 2007
  • Sweetpotato shoot tops (leaves, tips and petioles) are known to be very useful parts as vegetables because of their high nutritive values and great biomass yield. In this study, the phenolic compound contents, antibacterial activity, mutagenic activity, and antimutagenic activity were investigated in sweetpotato tips that were 10-15cm of shoot top including stems, petioles and tender leaves after sprout of storage roots. The study was done by extracting sweetpotato tips with 80% ethanol and the ethanol fraction was re-extracted with hexane, chloroform, ethyl acetate, butanol and water. In ethyl acetate and butanol fractions, total phenolic compounds contained 95. 6mg/g extract and 69.3 mg/g extract, respectively, The antibacterial activity was measured using the paper disk method with concentrations of 1, 2, 5 and 10 mg/disk of butanol and ethyl acetate fractions against L. monocytogenes and S. Typhimurium strains. Higher doses of solvent extracts showed the higher antibacterial activities. In addition, 5, 10 and 20 mg/mL of the extracts were tested to determine the antibacterial activity in liquid culture. The sweetpotato leaf extract by ethyl acetate showed 1 log reduction compared to control after 24 hrs on Listeria monocytogenes, but 20 mg/ml of butanol extract completely inhibited the growth of the pathogen after 12 hrs. The extracts from ethyl acetate or butanol on Salmonella Typhimurium did less than 1 log reduction during cultivation compared to control. The numbers of S. Typhimirium TA98 and TA100 revertant colonies were 29-33 and 159-188 CFU/plate, respectively, indicating that solvent extracts were no mutagenic activity. The antimutagenic test was performed by adding direct mutagen 2-NF and MMS, and butanol and ethyl acetate showed antimutagenic effect. Thus, this study showed that sweetpotato tips had high phenolic contents and both antimicrobiol and antimutagenic properties. Sweetpotato tips would be good nutritive source because of their high nutrient content without any toxicity in consuming.

Comparison in Seed and Sprout Quality under Different Cropping Patterns in Mungbean (재배방식에 따른 녹두 종실과 나물의 품질변화)

  • Kim, Dong-Kwan;Son, Dong-Mo;Choi, Jin-Gyung;Shin, Hae-Ryong;Chon, Sang-Uk;Lee, Kyung-Dong;Jung, Ki-Yeol;Rim, Yo-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.212-218
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    • 2011
  • This study was performed to determine the relative quality of mungbeans harvested in bulk after applying a labor-saving cultivation (LSC) method, compared to mungbeans harvested three different times under the conventional cultivation condition. There was no significant difference in starch, crude protein, and vitexin or isovitexin content of seed according to the cropping system or harvest time. The mungbeans grown under the LSC method had the highest crude fat content, followed by mungbeans from the third-, the second- and the first-harvest mungbeans under the conventional cultivation. No significant difference was found in the composite ratio of saturated fatty acids to unsaturated fatty acids according to cropping system or harvest time. The second-harvest mungbeans grown under the conventional cultivation condition had 17 different types of fatty acids, while the third-harvest mungbeans grown under the conventional cultivation and those grown under the LSC condition had the fewest types of fatty acids with 12. Of the major saturated fatty acids, palmitic acid and arachidonic acid had the highest composite ratio in the first conventional cultivation followed by the second, the third and the LSC. However, stearic acid showed the opposite tendency. Of the major unsaturated fatty acids, linoleic acid had the highest composite ratio in the first conventional cultivation, followed by the second and third conventional cultivation and the LSC. Amylogram characteristics of the mungbeans were significantly different according to cropping system and harvest times. The mungbeans harvested after the first conventional cultivation had significantly higher pasting temperature, peak viscosity, holding strength viscosity, final viscosity and breakdown, while mungbeans harvested after the third conventional cultivation had significantly higher setback viscosity. In contrast, the mungbeans harvested under the LSC methods had a significantly lower amylogram value. When harvest rate, color values and amino acid content of sprout were measured, mungbeans grown under the LSC conditions had a low harvest rate of sprout, but had Hunter's color values and amino acid content of sprout similar to those of mungbeans grown under the conventional cultivation condition.