• Title/Summary/Keyword: N-functions

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A Study about TV viewing behavior through Multiple Media under Nscreen circumstances (N스크린 환경에서 다중미디어를 활용한 TV프로그램 이용행태 연구)

  • Kim, Jongha Bell
    • Cartoon and Animation Studies
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    • s.31
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    • pp.177-208
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    • 2013
  • This research analyzes the media which is simultaneously used with TV viewing, the behaviour of multiple media users, and the characteristics of TV programs under Nscreen circumstances. According to the results, TV provides stories and positions it as the main screen (84.5%, n=566), under the Nscreen circumstances. Personal media reproduces stories from TV by utilizing aspects of social media functions such as participation, open communication, community, connectivity, etc. The content is classified under three categories: 1) elicit emotion(point feeling), 2) information production, 3) participation, which is further divided into three subcategories; watch, wish, and result guarantee based on the level of interactivity. This inquiry is based upon diary survey and focus group interviews emphasized the role change of TV, specifically that TV takes a key role in generating original stories with the expansion of screen and social media. Even though users were able to watch the same TV programs at the same time and same place, the different methods of viewing: smartphone, tablet PC, web based mp3 and etc., it reproduced different stories and elicited different characteristics from its TV viewers.

Effect of Ketamine on the Oxidative Burst Activity of Canine Peripheral Blood Leukocytes In Vitro (In Vitro에서 개 말초혈액 백혈구의 순간산소과소비현상에 대한 케타민의 효과)

  • Kim, Min-Jun;Kang, Ji-Houn;Yang, Mhan-Pyo
    • Journal of Veterinary Clinics
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    • v.23 no.4
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    • pp.393-399
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    • 2006
  • Ketamine, one of general anesthetics for human and veterinary use, is a non-competitive N-methyl-D-aspartate (NMDA) receptor antagonist which interferes with the action of excitatory amino acids. It has been reported to impair various leukocyte functions. In this study, the effect of ketamine on the oxidative burst activity (OBA) of canine peripheral blood leukocytes was examined. The OBA of canine peripheral blood phagocytes was analyzed by flow cytometry system. Ketamine at higher concentration such as $1,000{\mu}M$ exhibited a low viability of leukocytes. Thus, ketamine was used at concentration of 10 to $500{\mu}M$ showing no cytotoxic effect and high cell viability. The OBA of leukocytes in the presence or absence of latex beads was analyzed by addition of dihydrorhodamine 123. The direct treatment of ketamine revealed the inhibitory effect on the OBA of peripheral blood polymorphonuclear cells (PMN) and monocyte-rich cells but not peripheral blood mononuclear cells (PBMC) in the presence of latex beads. However, when latex beads were not added to PMN, its OBA was not inhibited by ketamine. The OBA of PMN and monocyte-rich cells but not PBMC in the presence of latex beads was also inhibited by culture supernatant from ketamine-treated- PBMC but not -PMN. But the OBA of PMN in the absence of latex beads was not inhibited by culture supernatant from PBMC treated with ketamine. Therefore, these results suggested that ketamine has the inhibitory effect on the OBA of canine peripheral blood phagocytes such as neutrophils and monocytes during phagocytic response.

Dna2 Helicase/endonuclease Interacts with a Novel Protein YHR122W Protein in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Dna2 helicase/endonuclease와 YHR122W 단백질의 상호작용)

  • Lee, Hyun-Sun;Choi, Do-Hee;Kwon, Sung-Hoon;Kim, Na-Yeon;Lee, In-Hwan;Kim, Hyun-Jung;Bae, Sung-Ho
    • Korean Journal of Microbiology
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    • v.42 no.1
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    • pp.1-6
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    • 2006
  • Saccharomyces cerevisiae Dna2 helicase/endonuclease plays an essential role in removing DNA primers during Okazaki fragment processing in eukaryotic DNA replication. Genome-wide scale co-immunoprecipitation experiments predicted that Dna2 interacts with a novel protein YHR122W (1). In this study, we observed that overexpression of YHR122W gene suppressed the temperature-sensitive phenotype of $dna2\Delta405N$ mutation. To investigate direct interaction between these two proteins, a histidine-tagged recombinant YHR122W protein was expressed and purified from E. coli. Physical interaction between the purified YHR122W and Dna2 proteins was detected by enzyme-linked immunosorbent assays. Further more, the complex formation was most efficient at physiological salt concentration, 150 mM NaCl. The genetic and physical interactions between YHR122W and Dna2 shown in this study suggest that the biological functions of these two proteins may be closely related each other.

Isolation and Characterization of a Formate Dehydrogenase cDNA in Poplar (Populus alba ${\times}$ P. glandulosa) (현사시나무에서 Formate Dehydrogenase cDNA의 분리와 특성 구명)

  • Bae, Eun-Kyung;Lee, Hyoshin;Lee, Jae-Soon;Choi, Young-Im;Yoon, Seo-Kyung;Eo, Soo Hyung
    • Journal of Korean Society of Forest Science
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    • v.102 no.3
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    • pp.331-337
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    • 2013
  • Formate dehydrogenase (FDH), catalyzing the oxidation of the formate ion to carbon dioxide, is known as the stress protein in response to drought, low temperature and pathogen infection. To study the functions of FDH in poplar (Populus alba ${\times}$ P. glandulosa), we isolated a FDH cDNA (PagFDH1) and examined its expressional characteristics. The PagFDH1 is 1,499 base pairs long and encodes a putative 388 amino acid protein with an expected molecular mass of 42.5 kDa. The PagFDH1 protein has N-terminal mitochondria signal peptide and $NAD^+$ binding domain. Southern blot analysis indicated that a single copy of the PagFDH1 is present in the poplar genome. PagFDH1 is expressed highly in the suspension cells (especially in the lag and early exponential phases) and moderately in roots, flowers and leaves. ABA-mediated enhanced expression of PagFDH1 in response to drought and salt stress treatments indicates that the gene product could play an important role in the development of stress resistant trees.

Application of Echo-Sounder Monitoring Technique as Ecological Impact Assessments of Fish on Artificial Weir Construction (인공보의 어류생태영향 평가를 위한 Echo-sounder 모니터링 기법 적용)

  • Han, Jeong-Ho;Lee, Jae Hoon;Choi, Ji-Woong;Lim, Byung Jin;Park, Jong-Hwan;An, Kwang-Guk
    • Journal of Environmental Impact Assessment
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    • v.21 no.6
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    • pp.859-877
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    • 2012
  • In this study, Echo-sounder($E_s$) monitoring methodology was applied to test environmental impacts of artificial weirs(SCW and JSW) in the Yeongsan River on fish community and habitat structures at first time, and was compared with conventional fish sampling methodologies($C_s$). For the Es monitoring in the fields, parallel transect methods was employed in determining the survey distance interval with every 125 m within the upper-lower 2 km of the weirs. Four different fish sampling gears such as casting nets, kick nets, fyke nets, and gill nets were used for applications of $C_s$ monitoring methodology. According to the Echo-sounder monitoring, fish density, expressed as a number of individuals per square meter, along the longitudinal axis of the weir was significantly greater(JSW, t = 3.506, n = 30, p < 0.001) in the down-river reach of the weir than in the up-river reach. Also, fish density along the vertical water column was highest at mid-depths of Seungchon weir, which has simple habitat substrates, while fish density was highest at hypolimnetic depth of Juksan weir. According to fish sampling by the $C_s$ methodology, the fish compositions decreased as the river goes upward, and significant differences(JSW, t = 0.248, n = 30, p < 0.05) in the compositions of fish species occurred between up-reach and down-reach of the weirs. The dominant species near the two weirs were Opsarichthys uncirostris amurensis, Hemiculter eigenmanni and Coilia nasus. Overall, our fish and habitat data, based on $E_s$ and $C_s$ monitoring methodologies, suggest that the weirs disturbed the rivers due to initial habitat disturbances by the weir constructions as well as the barrier roles of weirs on fish passage and migrations. More long-term scientific and systematic fish impact monitoring and assessments($E_s$ and $C_s$) are required in the future to predict changes of ecological structures and functions on the constructions of the weirs.

Forecasting the Sea Surface Temperature in the Tropical Pacific by Neural Network Model (신경망 모델을 이용한 적도 태평양 표층 수온 예측)

  • Chang You-Soon;Lee Da-Un;Seo Jang-Won;Youn Yong-Hoon
    • Journal of the Korean earth science society
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    • v.26 no.3
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    • pp.268-275
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    • 2005
  • One of the nonlinear statistical modelling, neural network method was applied to predict the Sea Surface Temperature Anomalies (SSTA) in the Nino regions, which represent El Nino indices. The data used as inputs in the training step of neural network model were the first seven empirical orthogonal functions in the tropical Pacific $(120^{\circ}\;E,\;20^{\circ}\;S-20^{\circ}\;N)$ obtained from the NCEP/NCAR reanalysis data. The period of 1951 to 1993 was adopted for the training of neural network model, and the period 1994 to 2003 for the forecasting validation. Forecasting results suggested that neural network models were resonable for SSTA forecasting until 9-month lead time. They also predicted greatly the development and decay of strong E1 Nino occurred in 1997-1998 years. Especially, Nino3 region appeared to be the best forecast region, while the forecast skills rapidly decreased since 9-month lead time. However, in the Nino1+2 region where they are relatively low by the influence of local effects, they did not decrease even after 9-month lead time.

The Impact of Feeding Diets of High or Low Energy Concentration on Carcass Measurements and the Weight of Primal and Subprimal Lean Cuts

  • Schinckel, A.P.;Einstein, M.E.;Jungst, S.;Matthews, J.O.;Fields, B.;Booher, C.;Dreadin, T.;Fralick, C.;Tabor, S.;Sosnicki, A.;Wilson, E.;Boyd, R.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.4
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    • pp.531-540
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    • 2012
  • Pigs from four sire lines were allocated to a series of low energy (LE, 3.15 to 3.21 Mcal ME/kg) corn-soybean meal-based diets with 16% wheat midds or high energy diets (HE, 3.41 to 3.45 Mcal ME/kg) with 4.5 to 4.95% choice white grease. All diets contained 6% DDGS. The HE and LE diets of each of the four phases were formulated to have equal lysine:Mcal ME ratios. Barrows (N = 2,178) and gilts (N = 2,274) were fed either high energy (HE) or low energy (LE) diets from 27 kg BW to target BWs of 118, 127, 131.5 and 140.6 kg. Carcass primal and subprimal cut weights were collected. The cut weights and carcass measurements were fitted to allometric functions (Y = A $CW^B$) of carcass weight. The significance of diet, sex or sire line with A and B was evaluated by linearizing the equations by log to log transformation. The effect of diet on A and B did not interact with sex or sire line. Thus, the final model was cut weight = (1+$b_D$(Diet)) A($CW^B$) where Diet = -0.5 for the LE and 0.5 for HE diets and A and B are sire line-sex specific parameters. Diet had no affect on loin, Boston butt, picnic, baby back rib, or sparerib weights (p>0.10, $b_D$ = -0.003, -0.0029, 0.0002, 0.0047, -0.0025, respectively). Diet affected ham weight (bD = -0.0046, p = 0.01), belly weight (bD = 0.0188, p = 0.001) three-muscle ham weight ($b_D$ = -0.014, p = 0.001), boneless loin weight (bD = -0.010, p = 0.001), tenderloin weight ($b_D$ = -0.023, p = 0.001), sirloin weight ($b_D$ = -0.009, p = 0.034), and fat-free lean mass ($b_D$ = -0.0145, p = 0.001). Overall, feeding the LE diets had little impact on primal cut weight except to decrease belly weight. Feeding LE diets increased the weight of lean trimmed cuts by 1 to 2 percent at the same carcass weight.

Validation of RESPECT-Music With a Korean Sample (한국판 음악 기능 척도의 타당화와 정서적 적응과의 관계)

  • Lee, Jung Yun;Kim, Minhee
    • Journal of Music and Human Behavior
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    • v.14 no.2
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    • pp.45-70
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    • 2017
  • The purpose of this study was to validate the Korean version of RESPECT-Music, which measures personal, social and cultural function of music, and to examine the correlation between the measured RESPECT data and the data measured from other scales for emotions. A survey was conducted with two separate groups of undergraduate students. Exploratory factor analysis with sample A (N=212) and confirmatory factor analysis and correlation analyses with sample B (N=296) were conducted. The result of exploratory factor analysis generated 10 factors as influential factors in music use, which was the similar results to the original scale: background, values, focus, dancing, family bonding, cultural identity, political attitudes, venting, emotional expression and social bonding. In the confirmatory factor analysis, this 35-item measurement was found to obtain adequate internal consistency and reliability. In addition, the correlations were found with other scales measuring emotional adjustment. Specifically, RESPECT showed a positive correlation with scales for positive affect, reappraisal, negative mood regulation and repair. Among the generated factors as music function, dancing was highly correlated with emotional adjustment, while political attitudes was negatively correlated with emotional adjustment. The results indicate that music use in our everyday lives is intercorrelated with the intrapersonal and interpersonal motives and emotional adjustment, while the function of music that influences cultural identity was not associated with the level of emotional adjustment. Implications for future studies were also suggested.

A Study on Suspension Optimization of the Korean Personal Rapid Transit Vehicle (한국형 PRT차량의 현가장치 최적화 연구)

  • Kim, Hyun Tae;Kim, Jun Woo;Cho, Jeong Gil;Koo, Jeong Seo;Kang, Seokwon;Jeong, Raggyo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.3
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    • pp.317-326
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    • 2016
  • In this study, running stability and ride quality analyses, applying the 'ISO 3888 (double lane change)' and 'ISO 2631-1' (mechanical vibration and shock) tests, were performed for the suspension optimization of the Korean personal rapid transit (PRT) vehicle. The suspension optimization results for running stability and ride quality were derived by applying the multiresponse surface method. From the comparisons of the optimization results for different ratios of the objective functions of running stability and ride quality, we derived the best objective function ratio of 3.9-to-6.1 to improve both the running stability and the ride quality. With the optimized results, the suspension stiffness became 30.68 N/mm, between the value of the $S_2$ and $S_3$ models, and the damping coefficient equaled that of the $D_1$ model. When compared with the suspension of the current PRT vehicle, the roll angle, yaw rate, sideslip angle, and ride comfort were improved by 0.37, 0.37, 2.8, and 5, respectively.

Characterization of in vitro Growth and Differentiation of an Albino Mutant of Nicotiana tobacum L. (Albino 담배 변이체의 기내 생장과 기내 분화의 특성)

  • ;;;;;;Yoshida Shigeo
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.197-203
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    • 1999
  • The albino plants of tobacco (Nicotiana tobacum L. cv. BY-4) were isolated from seed populations that were induced by heavy-ion ($^{14}N$) beam irradiation to proembryo and the in vitro growth and differentiation have been characterized. The in vitro cultured albino plants showed significant reduction of chlorophyll content and possessed larger number of stomata on both upper and lower epidermis than that of wild-type plants. Stem growth of the mutants remained dwarfed, however, the internode recovered its normal length after GA$_3$ treatment (10.0mg/L) on the MS medium containing sucrose under continuous light. When explants of leaf blades of albino plants were cultured, multiple shoots formed directly on MS medium containing 1.0mg/L of BAP or kinetin and a large number of calli were induced on the MS medium containing 1.0mg/L NAA or 1.0 mg/L 2,4-D. The albino calli regenerated multiple albino plantlets in the MS medium containing 0.1mg/L NAA + 1.0 mg/L BAP. No significant differences between the wild-type and albino plants were detected in the multiple shoot induction, callus formation from the explants and the plantlets regeneration from calli. In addition, albino plants have a similar organogenesis Pattern to that of the wild-type in the media with different combinations of NAA (0 to 5.0mg/L) and BAP (0 to 5.0mg/L) treatment. These results indicate that the albino mutant has the same normal regeneration ability as that of wild-type, although the mutant has lost functions in photosynthesis, such as pigmentation.

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