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Dna2 Helicase/endonuclease Interacts with a Novel Protein YHR122W Protein in Saccharomyces cerevisiae  

Lee, Hyun-Sun (Department of Biological Sciences, College of Natural Science, Inha University)
Choi, Do-Hee (Department of Biological Sciences, College of Natural Science, Inha University)
Kwon, Sung-Hoon (Department of Biological Sciences, College of Natural Science, Inha University)
Kim, Na-Yeon (Department of Biological Sciences, College of Natural Science, Inha University)
Lee, In-Hwan (Department of Biological Sciences, College of Natural Science, Inha University)
Kim, Hyun-Jung (Department of Biological Sciences, College of Natural Science, Inha University)
Bae, Sung-Ho (Department of Biological Sciences, College of Natural Science, Inha University)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 1-6 More about this Journal
Abstract
Saccharomyces cerevisiae Dna2 helicase/endonuclease plays an essential role in removing DNA primers during Okazaki fragment processing in eukaryotic DNA replication. Genome-wide scale co-immunoprecipitation experiments predicted that Dna2 interacts with a novel protein YHR122W (1). In this study, we observed that overexpression of YHR122W gene suppressed the temperature-sensitive phenotype of $dna2\Delta405N$ mutation. To investigate direct interaction between these two proteins, a histidine-tagged recombinant YHR122W protein was expressed and purified from E. coli. Physical interaction between the purified YHR122W and Dna2 proteins was detected by enzyme-linked immunosorbent assays. Further more, the complex formation was most efficient at physiological salt concentration, 150 mM NaCl. The genetic and physical interactions between YHR122W and Dna2 shown in this study suggest that the biological functions of these two proteins may be closely related each other.
Keywords
DNA replication; Dna2; Okazaki fragment processing; YHR122W;
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