• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제31권2호
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• pp.143-149
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• 2005

Objects : With the advancement of tissue engineering techniques, the effort to develop bioartificial mucosa have been actively delivered. The problem we met with this technique is the lack of mechanical strength between kerationocyte layer and dermal layer, where in the normal skin and mucosa, they are tightly bound with rete ridge structure. The purpose of this study is to understand the 2D and 3D structure of rete ridge of mucosa and skin paddle for rendering more biomimetic structure to the artificial mucosa. Materials and Methods : Oral mucosa and skin from the patients who received the oral surgery and maxillofacial reconstruction were harvested. The epidermis was separated from the dermis after treating with dispase for 12-16 hours. H&E staining was performed for 2D(dimensional) structure study and confocal LASER and SEM study were performed for 3D structure. Mean height(Sc) and arithmetic mean deviation(Sa) of all surface height were calculated. Results : The average height of rete ridge of skin flap was between $67.14{\mu}m$ and $194.55{\mu}m$. That of oral mucosa was between $146.26{\mu}m$ and $167.51{\mu}m$. Pressure bearing area and attached gingiva of oral mucosa showed deeper rete ridges. Conclusion : To obtain the adequate strength of artificially cultured keratinocyte skin and mucosa flap, it is necessary to imitate the original skin and mucosa structure, especially rete ridge. Through this study, 2D and 3D rete ridge structure of normal mucosa and skin was obtained. These results can be used as basis for substrate morphology for keratinocytes culture.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.53-73
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• 1996

• 생명과학회지
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• 제28권9호
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• pp.1081-1091
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• 2018

자일렌이 흡입된 흰쥐 후각점막의 구조와 복합당질 특성을 조사할 목적으로 Sprague-Dawley계 수컷 흰쥐를 사용하여 300 ppm의 자일렌을 하루에 5시간씩 5일간 흡입시키고 30일까지 회복시켰다. 자일렌 흡입에 대한 후각점막의 형태적 변화를 비교하였고, PSA, UEA I, PHA-L, GSL I B4, GSL I, PNA, ECL, SBA, GSL II 및 sWGA의 10종류 렉틴을 이용한 렉틴조직화학을 실시하여 복합당질의 특성을 비교하였다. 자일렌 흡입 후 20일까지 후각상피는 상피세포의 수와 상피의 높이가 감소되는 퇴행성 변화를 보였다. 대조군에서 후각상피의 후각신경세포는 PSA, UEA I, PNA, SBA 및 sWGA에서, 지지세포는 PSA, PHA-L, GSL I, PNA, ECL, SBA, GSL II 및 sWGA에서, 그리고 후각샘은 10종류의 모든 렉틴에서 양성반응이 관찰되었다. 실험군에서 후각신경세포는 PSA, PNA 및 SBA에서 반응성이 감소하였고, 지지세포는 PSA, PNA, SBA 및 GSL II에서 반응성이 감소하였으며, 후각샘은 PSA, UEA I, GSL I 및 sWGA에서 그 반응성이 감소하였다. 결론적으로, 자일렌에 노출된 후각점막은 구조와 복합당질양상이 대조군에 비하여 많은 변화를 나타내었다. 이상의 결과들은 후각점막의 복합당질 당잔기가 자일렌 흡입 후에 변화할 수 있음을 시사한다.

• 대한방사선치료학회지
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• 제7권1호
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• pp.92-96
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• 1995

All patients who will Undergo irraidiation of the oral cavity cancer will need dental before and during Radiotherapy. The extent of the region and the presence of numerous critical normal tissues(mucosa, gingiva, teeth and the alveolar ridge, alveolar bony structure, etc) in the oral cavity area, injury to which could result in serious functional impairment. Therefore I evaluate the Usefulness of custom-made intraoral shielding device before and during Radiotherapy in oral cavity cancer. Materials and Methods(1) : Manufacture process of Custom-made intraoral shielding device Containing Cerroband. A. Acquisition of impression B. Matrix Constitution C. Separation by Separator D. Sprincle on method E. Trimming F. Spacing G. Fill with Cerroband Materials and Methods (2) A. Preannealing B. TLD Set up C. Annealing D. TLD Reading = Results = Therefore dosimetric characteristics in oral cavity by TLD Compared to isodose curve dose distribution Ipsilateral oral mucosa, Contralateral oral mucosa, alveolar ridge, tongue, dose was reduced by intraoral shielding device containning Cerroband technique Compard to isodose plan = Conclusions = The custom-made intra-oral shielding device containing Cerroband was useful in reducing the Contralateral oral mucosa dose and Volume irradiated.

• The Korean Journal of Physiology and Pharmacology
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• 제20권2호
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• pp.147-152
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• 2016

Present study aimed to investigate the effect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-${\alpha}$ levels were measured. Expression of intestinal TNF-${\alpha}$ and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-${\alpha}$ leves were significantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment significantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the effect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this effect may be partly attributed to the TNF-${\alpha}$ related pathway.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제49권5호
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• pp.304-307
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• 2023

Venous malformation (VM) is a benign lesion of blood vessels caused by an error in vascular morphogenesis during the embryologic phase. This entity mostly affects the head and neck region, including the lips, tongue, buccal mucosa, gingiva, or palate. VM may cause functional and aesthetic impairments. The anatomical structure and shape of the lips provide an important aesthetic accent for an individual. Therefore, management of VM in the lip area without postoperative defects or scarring is challenging. In this brief communication article, we present a conservative approach to lip VM in a nine-year-old boy using a bleomycin injection that had good aesthetic and functional outcomes. Injection of 2 mL of 1/10 of 15 mg bleomycin in a saline dilution into the lip mucosa may present a drug reaction as a white plaque and reddish owl eye lesion that takes up to three weeks to resolve without a scar. It is important to recognize the characteristics and self-limiting nature of postoperative bleomycin complications to avoid unnecessary treatment.

• 대한임상검사과학회지
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• 제36권2호
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• pp.173-177
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• 2004

This study is performed in order to clarify the histochemical structure, the distribution of mucous cell and goblet cell, and the histochemical properties of the mucosubstances in the middle region of intestinal mucosa and rectum of Raja kenojei. In the H&E stain, distribution of the mucous cells and acidophilic cells were a more compacted middle portion than other regions of intestine, but the former was more than the latter in the number of mucous cells to rectum. The mucosubstances were stained with aldehyde fuchsin pH 1.7-alcian blue(pH 2.5) stain and then compared to distribution of the mucosubstances used in image and microscope technology(IMT-Size5). The middle intestine of Raja kenojei was composed of mucous cells having only large amounts of mucosubstances in the distal region was much more than that of proximal region. It was two types of mucous cells to rectum, one type was the same as proximal intestine while the other had small amounts of weakly sulfated and large amounts of carboxylated mucins.

• 한국어류학회지
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• 제18권1호
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• pp.27-35
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• 2006

한국산 칼납자루의 암컷은 산란시기가 되면 배지느러미 기부 부근에 돌출되어 신장되어 나타나는 배측융기가 형성된다. 이러한 배측융기는 직장(항문)부분과 내측산란관으로 구성되었다. 직장은 점막층, 점막하층, 근육층과 이들을 둘러싸는 다층의 표층으로 구성되어 있으며 특히 점막층에는 산성 점액다당류인 점액세포가 존재한다. 한편 내측산란관은 직장과 비슷한 구조를 가지고 있으나 점막층에는 점액세포가 존재하지 않는 특징을 보였다. 한편 외측산란관은 배측융기와 연결되지 않아 가늘고, 긴 관의 형태를 보이고 있으나 내측산란관과 달리 근육층이 존재하지 않아 점막층, 점막하층, 다층의 표피층으로 구성되어 있다. 이러한 결과로 보아 근육층을 가지는 내측산란관은 성숙란을 외측산란관으로 내보기 위해 연동운동과 관련있는 반면에 외측산란관은 근육층이 존재하지 않아 내측산란관으로부터 방출된 성숙란을 단지 이매패 속으로 전달하는 단순한 관의 역할을 하는 것으로 생각된다.

• Journal of Periodontal and Implant Science
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• 제26권2호
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• pp.522-530
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• 1996

Most dentists are very interested in laser therapy on the intraoral soft tissue lesions because they want to accomplish the analgesic and aseptic surgery with little or no bleeding. In order to determine the difference of pain threshold according to different gingival tissues with or without inflammation, 25 patients with inflammatory periodontal disease and 10 volunteers with good general and oral health were selected as the inflamed group and the normal group, respectively. Interdental papilla, marginal gingiva, attached gingiva, and alveolar mucosa were irradiated by the contact delivery($300{\mu]m$ fiber optic, for 5 seconds) of a pulsed Nd:YAG laser(EN.EL.EN06O, Italy). And the laser power was gradually increased from 0.5W by the increment of 0.1W. The highest laser power was recorded as the first painful power when the painful gesture was recognized at first. The difference of the first painful power of laser according to different gingival tissues with or without inflammation was statistically analyzed by paired t-test in MICROSTAT program. Following results were obtained: 1. In the comparison related with the inflammation, the first painful power was significantly lower in the inflamed group than in the normal group, regardless of interdental papilla and marginal gingiva(p<0.05). 2. In the comparison related with the tissue structure, the first painful. power was significantly lower in alveolar mucosa than in attached gingiva(p<0.05). The results suggest that, for the painless therapy by a pulsed-Nd:YAG laser irradiation, the laser surgery over 2.0W of power should be necessarily accomplished under the local anethesia, and the local anesthesia should be considered according to the degree of inflammation, the tissue structure, and the purpose of laser therapy.

• The Korean Journal of Physiology and Pharmacology
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• 제14권5호
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• pp.317-324
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• 2010

We elucidated the distribution of interstitial cells of Cajal (ICC) in human stomach, using cryosection and $c-Kit$ immunohistochemistry to identify $c-Kit$ positive ICC. Before $c-Kit$ staining, we routinely used hematoxylin and eosin (HE) staining to identify every structure of human stomach, from mucosa to longitudinal muscle. HE staining revealed that the fundus greater curvature (GC) had prominent oblique muscle layer, and $c-Kit$ immunostaining $c-Kit$ positive ICC cells were found to have typical morphology of dense fusiform cell body with multiple processes protruding from the central cell body. In particular, we could observe dense processes and ramifications of ICC in myenteric area and longitudinal muscle layer of corpus GC. Interestingly, $c-Kit$ positive ICC-like cells which had morphology very similar to ICC were found in gastric mucosa. We could not find any significant difference in the distribution of ICC between fundus and corpus, except for submucosa where the density of ICC was much higher in gastric fundus than corpus. Furthermore, there was no significant difference in the density of ICC between each area of fundus and corpus, except for muscularis mucosa. Finally, we also found similar distribution of ICC in normal and cancerous tissue obtained from a patient who underwent pancreotomy and gastrectomy. In conclusion, ICC was found ubiquitously in human stomach and the density of ICC was significantly lower in the muscularis mucosa of both fundus/corpus and higher in the submucosa of gastric fundus than corpus.