[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4196/kjpp.2016.20.2.147

Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-α related mechanism

Tian, Shuying (Department of Gastroenterology, Cangzhou Central Hospital)
Guo, Ruixue (Department of Gastroenterology, Cangzhou Central Hospital)
Wei, Sichen (Department of Gastroenterology, Cangzhou Central Hospital)
Kong, Yu (Department of Gastroenterology, Cangzhou Central Hospital)
Wei, Xinliang (Department of Gastroenterology, Cangzhou Central Hospital)
Wang, Weiwei (Department of Gastroenterology, Cangzhou Central Hospital)
Shi, Xiaomeng (Department of Gastroenterology, Cangzhou Central Hospital)
Jiang, Hongyu (Department of Gastroenterology, Cangzhou Central Hospital)

Publication Information

The Korean Journal of Physiology and Pharmacology / v.20, no.2, 2016 , pp. 147-152 More about this Journal

Abstract

Present study aimed to investigate the effect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)- ${\alpha}$ levels were measured. Expression of intestinal TNF- ${\alpha}$ and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF- ${\alpha}$ leves were significantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment significantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the effect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this effect may be partly attributed to the TNF- ${\alpha}$ related pathway.

Keywords

Curcumin; Intestine; Intestinal mucosa barrier; Intestinal permeability; Ischemia/reperfusion (I/R) injury; Tight junction protein (ZO-1);

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)

Reference
Cited By KSCI

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