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http://dx.doi.org/10.4196/kjpp.2010.14.5.317

Regional Distribution of Interstitial Cells of Cajal, (ICC) in Human Stomach  

Yun, Hyo-Yung (Department of Surgery, School of Medicine, Chungbuk National University)
Sung, Ro-Hyun (Department of Pathology, School of Medicine, Chungbuk National University)
Kim, Young-Chul (Department of Physiology, School of Medicine, Chungbuk National University)
Choi, Woong (Department of Pharmacology, School of Medicine, Chungbuk National University)
Kim, Hun-Sik (Department of Pharmacology, School of Medicine, Chungbuk National University)
Kim, Heon (Department of Preventive Medicine, School of Medicine, Chungbuk National University)
Lee, Gwang-Ju (Department of Pathology, School of Medicine, Chungbuk National University)
You, Ra-Young (Department of Physiology, School of Medicine, Chungbuk National University)
Park, Seon-Mee (Department of Internal Medicine, School of Medicine, Chungbuk National University)
Yun, Sei-Jin (Department of Internal Medicine, School of Medicine, Chungbuk National University)
Kim, Mi-Jung (Department of Pediatric, College of Medicine, School of Medicine, Chungbuk National University)
Kim, Won-Seop (Department of Pediatric, College of Medicine, School of Medicine, Chungbuk National University)
Song, Young-Jin (Department of Surgery, School of Medicine, Chungbuk National University)
Xu, Wen-Xie (Department of Physiology, School of Medicine, Shanghai Jiaotong University)
Lee, Sang-Jin (Department of Physiology, School of Medicine, Chungbuk National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.14, no.5, 2010 , pp. 317-324 More about this Journal
Abstract
We elucidated the distribution of interstitial cells of Cajal (ICC) in human stomach, using cryosection and $c-Kit$ immunohistochemistry to identify $c-Kit$ positive ICC. Before $c-Kit$ staining, we routinely used hematoxylin and eosin (HE) staining to identify every structure of human stomach, from mucosa to longitudinal muscle. HE staining revealed that the fundus greater curvature (GC) had prominent oblique muscle layer, and $c-Kit$ immunostaining $c-Kit$ positive ICC cells were found to have typical morphology of dense fusiform cell body with multiple processes protruding from the central cell body. In particular, we could observe dense processes and ramifications of ICC in myenteric area and longitudinal muscle layer of corpus GC. Interestingly, $c-Kit$ positive ICC-like cells which had morphology very similar to ICC were found in gastric mucosa. We could not find any significant difference in the distribution of ICC between fundus and corpus, except for submucosa where the density of ICC was much higher in gastric fundus than corpus. Furthermore, there was no significant difference in the density of ICC between each area of fundus and corpus, except for muscularis mucosa. Finally, we also found similar distribution of ICC in normal and cancerous tissue obtained from a patient who underwent pancreotomy and gastrectomy. In conclusion, ICC was found ubiquitously in human stomach and the density of ICC was significantly lower in the muscularis mucosa of both fundus/corpus and higher in the submucosa of gastric fundus than corpus.
Keywords
Human stomach; Interstitial cells of Cajal (ICC); c-Kit; Fundus; Corpus; Greater and Lesser curvature;
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