• Title/Summary/Keyword: Morphological characterization

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Identification and Characterization of Unreported Penicillium Species in Korea

  • Choi, Doo-Ho;Kim, Young-Guk;Lee, In-Seon;Hong, Seung-Bum;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.445-456
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    • 2020
  • Fungal species belonging to the genus Penicillium are indigenous to a wide range of natural environments. Since the first published reports detailing the discovery of Penicillium, new species have been identified in various countries, including Korea. We present here a full characterization of five species of Penicillium that were previously unreported in Korea. Based on the morphologic characteristics and sequences of genes encoding fungal ��-tubulin and calmodulin, we identified five Penicillium species, including P. hetheringtonii, P. sublectaticum, and P. jacksonii, which have been unrecorded in Korea, and confirming P. maximae and P. cremeogriseum, as the endophytic fungi isolated in Gyeongsang province, Korea. In this article, we provide detailed morphological descriptions of these fungal species.

Morphological and Genetic Characterization of Penicillium spp. associated with post - harvest decay of fruits. (oral)

  • Oh, S.Y.;Yu, S.H.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.115.1-115
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    • 2003
  • Post-harvest decay, caused by Penicillium spp. is a serious problem of fruits worldwide. Morphological characteristics and molecular markers were used to characterize 22 Penicillium isolates from apples, 18 isolates from pears, 60 from oranges and 18 from grapes and 23reference isolates representing related Penicillium spp. to assess their diversity and resolve their taxonomy. Based on morphological and physiological characteristics, the isolates were grouped as identical or very similar to P. digitatum, P. italicum, P. ulaiense or very similar to P. crustosum, P. expansum, P. solitum and unidentified Penicillium spp. Based on sequence comparisons of ITS region, variable site were presented within and among the species, but there variation were not correlated with the species. Cluster analyses of AP-PCR fragment patterns using UP and L45 primer and the -tubulin gene sequence, the Penicillium species were segregated into distinct groups. Particularly. the -tubulin partial sequence data provided support for species concepts based on morphological and physiological characteristics.

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Polymorphic stages of the fresh water blue-green alga, Gomphosphaeria aponina

  • Dwivedi, V.K.;Tandon, Richa;Tiwari, G.L.
    • ALGAE
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    • v.25 no.3
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    • pp.115-120
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    • 2010
  • The natural growth of a population of Gomphosphaeria aponina Kutzing (Chroococcales, Cyanoprocaryota) was studied in a cemented freshwater tank in Allahabad, India. This population appeared to be a polymorphic species. Different species of the genus Gomphosphaeria have been segregated based on morphological features of colonies, cells and mucilage. However, these features are not well defined for different species. Our observations revealed many feature variations and, interestingly, certain features that have been described for different Gomphosphaeria species were seen in a single population. In this study, records of such variable morphological features were possible due to the availability of numerous specimens and continuous observations for more than two years. Further, this study revealed two points: (i) more detailed morphological studies are required both from nature as well as in culture to identify critical differences among the species, and (ii) molecular characterization of taxa appears to be necessary for final species settlement.

Morphological features of thermophilic activated sludge treating food industry wastewater in MBR

  • Ince, Mahir;Topaloglu, Alikemal;Ince, Elif
    • Membrane and Water Treatment
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    • v.9 no.1
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    • pp.33-42
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    • 2018
  • Microscopic examination of the activated sludge and morphological characterization of the flocs provides detailed information about the treatment process. The aim of this study is to investigate the morphological parameters of flocs obtained from a thermophilic jet loop membrane bioreactor (JLMBR) in different sludge retention times (SRTs), considering EPS and SMP concentration, hydrophobicity, zeta potential. The results showed that irregularity decreased with the increasing SRT. The compactness value was calculated to be less than 1 for all SRTs. However, the sludge had a more compact structure when the SRT increased. Zeta potential increased whereas hydrophobicity and floc size reduced, with increasing SRT. Furthermore, 2-D porosity calculated using the hole ratio was higher at greater SRTs. Hence, there was a significant correlation between the results obtained using the imaging technique and operation conditions of thermophilic JLMBR.

Codium fragile subsp. fragile (Suringar) Hariot in Tunisia: morphological data and status of knowledge

  • Cherif, Wafa;Ktari, Leila;Bour, Monia El;Boudabous, Abdellatif;Grignon-Dubois, Micheline
    • ALGAE
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    • v.31 no.2
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    • pp.129-136
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    • 2016
  • The Mediterranean Sea is currently facing dramatic changes and threats, including change in native species and accidental introductions. The introduced green alga Codium fragile subsp. fragile (Suringar) Hariot influences diversity and community structure in some parts of the world. This paper documents the distribution of this species in Tunisia and provides a morphological description of C. fragile subsp. fragile in Northern Tunisia. Results confirm the identity of Tunisian specimens as the invasive subspecies C. fragile subsp. fragile. This is the first morphological characterization of this subspecies in Tunisia.

Identification and Characterization of Unrecorded Aspergillus spp. in Korea

  • Choi, Doo-Ho;Yoon, Hyeok-Jun;Hong, Seung-Bum;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.1-10
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    • 2021
  • A survey of fungal diversity in Gyeongsang Province, Korea, revealed two previously unreported Aspergillus isolates, named KMG411 and KMG412. The phylogeny of the isolates was analyzed based on β-tubulin (BenA) and calmodulin (CaM) sequencing. Morphological analyses further identified the KMG411 and KMG412 as A. insuetus and A. nomius, respectively. Here we provide detailed morphological descriptions of the previously unrecorded Aspergillus species.

Sample Preparation for Microstructural Characterization of Ni-Yttria-Stabilized Zirconia Anodes

  • Sim, Soo-Man
    • Journal of the Korean Ceramic Society
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    • v.55 no.4
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    • pp.376-380
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    • 2018
  • Microstructural characterization of Ni-yttria-stabilized zirconia (YSZ) anodes using secondary electron images has been limited by a lack of contrast between Ni and YSZ phases. This paper reports a sample preparation method for obtaining secondary electron images that allow the detection of Ni, YSZ, and pore phases together. Ni-YSZ anode samples were obtained by reducing NiO-YSZ samples prepared by using the mixed oxide method. Colloidal silica polishing and electrolytic etching were performed on the Ni-YSZ samples. The morphological change of the sample surface after each polishing process is examined.

Recent Advances in Electrochemical Studies of π-Conjugated Polymers

  • Park, Su-Moon;Lee, Hyo-Joong
    • Bulletin of the Korean Chemical Society
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    • v.26 no.5
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    • pp.697-706
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    • 2005
  • We review the evolution of electrochemical studies of conducting polymers into the current state-of-the-art based primarily on our work. While conventional electrochemical experiments sufficed for the needs in the phase of studies of both electrochemical synthesis and characterization of conducting polymers, developments of various new experimental techniques have led to their introduction to this field for more refined information. As a result, the conventional electrochemical, spectroelectrochemical, electrochemical quartz crystal microbalance, impedance, and morphological as well as electrical characterization studies all made important contributions to a better understanding of the polymerization mechanisms and the conductive properties of these classes of polymers. From this review, we hereby expect that the electrochemical techniques will continue to play important roles in bringing this field to the practical applications such as nanoscale electronic devices.

Morphological and Genetic Characterization of Caffeine-Rich and -Poor Tea Tree (Camellia sinensis L.) Lines

  • Kim, Yong-Duck;Jeong, Mi-Jin;Song, Hyun-Jin;Yun, Seok-Rak;Heo, Chang-Mi;Kim, Chang-Soo;Moon, Hyun-Shik;Choi, Myung-Suk
    • Journal of agriculture & life science
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    • v.45 no.5
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    • pp.1-8
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    • 2011
  • In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines.

Phylogenetic and Morphological Characterization of Cladosporium perangustum Associated with Flyspeck on Shine Muscat Grapes in South Korea

  • Hassan, Oliul;Lim, Yang-Sook;Chang, Taehyun
    • Mycobiology
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    • v.49 no.2
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    • pp.183-187
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    • 2021
  • The Shine Muscat is a table grape, popular in South Korea for its unique mango-flavor taste. Flyspeck is a disease that is characterized by small, black, and circular specks on the grape cuticle was first observed in several commercial orchards in Sangju, South Korea, in August 2019. Here we identified the causal agent of flyspeck based on an advanced diagnosis approach, comprised of both morphological and molecular analyses. Morphological characteristics of the cultures isolated from grape flyspeck were identical to the fungus Cladosporium perangustum. The concatenated sequences of ITS, ACT, and EF1-α were used for molecular phylogenetic analysis, BLAST searches along with Bayesian inference-based phylogeny, confirmed that the causal agent of grape flyspeck is C. perangustum. The cultured fungal isolates also produced flyspeck symptoms on healthy fruits in pathogenicity tests. To the best of my knowledge, this is the first documented evidence of any Cladosporium sp. producing flyspeck symptoms on any plant.