Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Morphological and Genetic Characterization of Caffeine-Rich and -Poor Tea Tree (Camellia sinensis L.) Lines

  • Kim, Yong-Duck (Institute of Hadong Green Tea) ;
  • Jeong, Mi-Jin (Div. of Environ. Forest Sci., Gyeongsang National Univ. (Insti. of Agric. and Life Sci.)) ;
  • Song, Hyun-Jin (Div. of Environ. Forest Sci., Gyeongsang National Univ. (Insti. of Agric. and Life Sci.)) ;
  • Yun, Seok-Rak (Hanbaek Forest Health & Protection Institute) ;
  • Heo, Chang-Mi (Div. of Environ. Forest Sci., Gyeongsang National Univ. (Insti. of Agric. and Life Sci.)) ;
  • Kim, Chang-Soo (Dept. of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Moon, Hyun-Shik (Div. of Environ. Forest Sci., Gyeongsang National Univ. (Insti. of Agric. and Life Sci.)) ;
  • Choi, Myung-Suk (Div. of Environ. Forest Sci., Gyeongsang National Univ. (Insti. of Agric. and Life Sci.))
  • Received : 2011.05.23
  • Accepted : 2011.10.28
  • Published : 2011.10.31
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Abstract

In this study, 160 tea tree (Camellia sinensis L.) lines were classified by caffeine content using colorimetric methods. Among them, caffeine-rich lines (HR-78, HR-137, HR-82 and HR-123) and poor lines (HP-85, HP-88, HP-19, and HP-131) were selected. To know the difference in morphological and genetic characters between caffeine-rich and poor lines, we used leaf/shoot growth and RAPD methods. Cluster pattern of morphological characters (leaf width, leaf length, leaf area and shoot length) showed that shoot length was longer in caffein-rich lines than in -poor lines. In genetic analysis, amplified DNA bands having various sizes were detected in RAPD analysis where 30 random primers were used. However, the discriminated primer set that distinguish caffein-rich tree line from -poor lines was not found. These results can be used as the basic data to determine the morphological and genetic differences among caffein-rich and -poor lines.

Keywords

Acknowledgement

Supported by : Ministry for Food, Agriculture, Forestry and Fisheries

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