The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Characterization of Unrecorded Aspergillus spp. in Korea

  • Choi, Doo-Ho (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Yoon, Hyeok-Jun (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Hong, Seung-Bum (Korean Agricultural Culture Collection, National Academy of Agricultural Science, RDA) ;
  • Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University)
  • Received : 2020.10.20
  • Accepted : 2021.02.26
  • Published : 2021.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

A survey of fungal diversity in Gyeongsang Province, Korea, revealed two previously unreported Aspergillus isolates, named KMG411 and KMG412. The phylogeny of the isolates was analyzed based on β-tubulin (BenA) and calmodulin (CaM) sequencing. Morphological analyses further identified the KMG411 and KMG412 as A. insuetus and A. nomius, respectively. Here we provide detailed morphological descriptions of the previously unrecorded Aspergillus species.

Keywords

Acknowledgement

This study was supported by grants from the National Institute of Biological Resources (NIBR) and Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Environment (MOE) of the Republic of Korea NIBR201801208, the Ministry of Education (NRF-2016R1A6A1A05011910) and Research Institute for Dok-do and Ulleung-do Island of Kyungpook National University, Korea.

References

  1. Kirk PM, Cannon PF, David JC, Stalpers JA. Dictionary of the fungi. 9th ed. Wallingford: CABI Publishing; 2001.
  2. Berbee ML, Taylor JW. Dating the evolutionary radiations of the true fungi. Can J Bot 1993;71:1114-27. https://doi.org/10.1139/b93-131
  3. Gams W, Christensen M, Onions AH, Pitt JI, Samson RA. Infrageneric taxa of Aspergillus. Advances in Penicillium and Aspergillus systematics. In: Samson RA, Pitt JI, editors. Boston: Springer; 1985. p. 55-61.
  4. National Center for Biotechnology Information. GenBank overview [Internet]. Bethesda (MD): National Center for Biotechnology Information; 2015 [cited 2018 September 10]. Available from: https://www.ncbi.nlm.nih.gov/genbank/.
  5. Samson RA, Varga J. What is a species in Aspergillus? Med Mycol 2009;47:S13-20. https://doi.org/10.1080/13693780802354011
  6. Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Williams and Wilkins; 1965.
  7. Goldman GH, Osmani SA. The aspergilli: Genomics, medical aspects, biotechnology, and research methods. Boca Raton: CRC Press; 2008.
  8. Diba K, Kordbacheh P, Mirhendi SH, Rezaie S, Mahmoudi M. Identification of Aspergillus species using morphological characteristics. Pak J Med Sci 2007;23:867-72.
  9. Visagie CM, Hirooka Y, Tanney JB, Whitfield E, Mwange K, Meijer M, Amend AS, Seifert KA, Samson RA. Aspergillus, Penicillium, and Talaromyces isolated from in house dust samples collected around the world. Stud Mycol 2014;78:63-139. https://doi.org/10.1016/j.simyco.2014.07.002
  10. Nguyen TTT, Pangging M, Bangash NK, Lee HB. Five new records of the family Aspergillaceae in Korea, Aspergillus eruopaeus, A. pragensis, A. tennesseensis, Penicillium fluviserpens and P. scabrosum. Mycobiol 2020;48:81-94. https://doi.org/10.1080/12298093.2020.1726563
  11. Park MS, Chung D, Baek K, Lim YW. Three Unrecorded species belonging to Penicillium section Sclerotiora from marine environments in Korea. Mycobiol 2019;47:165-72. https://doi.org/10.1080/12298093.2019.1601330
  12. Choi DH, Kwon HJ, Kim MG, Kim DH, Kim YG, Kim JG. A-11: Diversity of endophytic fungi isolated from plant's roots in part of Korean Peninsula. Kor J Mycol 2019;31:74.
  13. Khan SA, Hamayun M, Kim HY, Yoon HJ, Seo JC, Choo YS, Lee IJ, Kim SD, Rhee IK, Kim JG. A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production. Biotechnol Lett 2009;31:283-7. https://doi.org/10.1007/s10529-008-9862-7
  14. Wang XC, Chen K, Zeng ZQ, Zhuang WY. Phylogeny and morphological analyses of Penicillium section Sclerotiora (Fungi) lead to the discovery of five new species. Sci Rep 2017;7:8233-46. https://doi.org/10.1038/s41598-017-08697-1
  15. Kil YJ, Eo JK, Eom AH. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor J Mycol 2009;37:130-3. https://doi.org/10.4489/KJM.2009.37.2.130
  16. Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CHW, Perrone G, Seifert KA, Varga J, Yaguchi T, Samson RA. Identification and nomenclature of the genus Penicillium. Stud Mycol 2014;78:343-71. https://doi.org/10.1016/j.simyco.2014.09.001
  17. Pitt JI. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. London: Academic Press; 1979.
  18. Kim HJ, Kim JS, Cheon KH, Kim DH, Seok SJ, Hong SB. Species list of Aspergillus, Penicillium, and Talaromyces in Korea, based on 'One Fungus One Name' system. Kor J Mycol 2016;44:207-19. https://doi.org/10.4489/KJM.2016.44.4.207
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  20. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  21. Samson RA, Varga J, Meijer M, Frisvad JC. New taxa in Aspergillus section Usti. Stud Mycol 2011;69:81-97. https://doi.org/10.3114/sim.2011.69.06
  22. Frisvad JC, Hubka V, Ezekiel CN, Hong SB, Novakova A, Chen AJ, Arzanlou M, Larsen TO, Sklenar F, Mahakarnchanakul W. Houbraken J. Taxonomy of Aspergillus section Flavi and their production of aflatoxins, ochratoxins and other mycotoxins. Stud Mycol 2019;93:1-63. https://doi.org/10.1016/j.simyco.2018.06.001
  23. Ropars J, Dupont J, Fontanillas E, Rodriguez de la Vega RC, Malagnac F, Coton M, Giraud T, Lopez-Villavicencio M. Sex in cheese: Evidence for sexuality in the fungus Penicillium roqueforti. PLoS ONE 2012;7:e49665. https://doi.org/10.1371/journal.pone.0049665
  24. Kurtzman CP, Horn BW, Hesseltine CW. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie van Leeuwenhoek 1987;53:147-58. https://doi.org/10.1007/BF00393843
  25. Samson RA, Varga J, Meijer M, Frisvad JC. New taxa in Aspergillus section Usti. Stud Mycol 2011;69:81-97. https://doi.org/10.3114/sim.2011.69.06