• BMB Reports
• /
• v.47 no.10
• /
• pp.593-598
• /
• 2014

RNA polymerase II carboxyl-terminal domain (RNAPII CTD) phosphatases are responsible for the dephosphorylation of the C-terminal domain of the small subunit of RNAPII in eukaryotes. Recently, we demonstrated the identification of several interacting partners with human small CTD phosphatase1 (hSCP1) and the substrate specificity to delineate an appearance of the dephosphorylation catalyzed by SCP1. In this study, using the established cells for inducibly expressing hSCP1 proteins, we monitored the modification of ${\beta}$-O-linked N-acetylglucosamine (O-GlcNAc). O-GlcNAcylation is one of the most common post-translational modifications (PTMs). To gain insight into the PTM of hSCP1, we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutinin-precipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the $Ser^{41}$ residue of hSCP1 as the O-GlcNAc modification site. These results suggest that hSCP1 may be an O-GlcNAcylated protein in vivo, and its N-terminus may function a possible role in the PTM, providing a scaffold for binding the protein(s).

• Journal of KSNVE
• /
• v.8 no.5
• /
• pp.841-848
• /
• 1998

In the gear manufacturing, tooth modification is usually applied for the prevention of tooth impact during the loading. In contrary, tooth profile error causes amplifying the whine noise which is cumbersome to reduce in the automobile gear box. So optimum quantity of the modifications must be obtained for the good performance in the vibrational sense. In this paper, a formulation to define the tooth curve by considering the profile manufacturing error and loading deformation of the gear tooth is suggested and the transmission error and loading deformation of the gear tooth is suggested and the transmission error with modified tooth in the gear system is evaluated. A pair of gear set is mathematically modelled. The equivalent excitation in the gear vibratonal model is formulated. For the experimental evaluaton on the derived transmission error function, a simple geared system is set up in which the gears are designed to give pre-designed tooth profile modification and manufactured by CNC Wire Cutting Machine. Under slow speed operaton, the transmission error of the gear pair is measured by using two rotational laser vibrometers, compared with the calculated one of which the result shows good agreement.

• Journal of Microbiology
• /
• v.40 no.3
• /
• pp.219-223
• /
• 2002

In order to investigate the function of Soo1p/${\alpha}$-COP during post-translational modification and intra-cellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/ or Ο-glycosylation. Analysis of cell wall proteins with antibodies against ${\beta}$-1,3-glucan and ${\beta}$-1,6-glucan revealed alteration of the linkage between cell wall proteins and ${\beta}$-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.

• Journal of Advanced Marine Engineering and Technology
• /
• v.23 no.4
• /
• pp.574-582
• /
• 1999

This paper discusses the multi-level optimization method in dynamic optimization problems through stiffened plate of ship structures. In structural optimization the computational cost increases rapidly as the number of design variables increases. And we need a great amount of cal-culation and time on problems of modified dynamic characteristics of large and complicated struc-tures. In this paper the multi-level optimization is proposed which decreases computational time and cost. the dynamic optimum designs of stiffened plate that control the natural frequency and minimize weight subjected to constraints condition are derived. The way to apply the multi-level optimization methods in this study follow: In the first step the dynamic characteristics is controlled for the two-dimensional model of stiffened plate by sensitivity analysis and quasi-least squares methods. In the second step the cross-section of the stiffener is decided so that the weight is minimized under needed constraints by the steepest descent or ascent method. In the third the three-dimensional model is made based on the results of the first step and the second step confirmation and finer tuning of the objective function are carried out. It is shown that the results are effective in the optimum modification for dynamic characteristics of the stiffened plate.

• Elastomers and Composites
• /
• v.44 no.3
• /
• pp.260-268
• /
• 2009

Modification of clay surface was attempted by treating the clay with bis[(3-triethoxysilylpropyl)tetra sulfide (TSS) to raise the hydrophobicity and to induce a chemical reaction between the clay and the high temperature vulcanization-type silicone rubber matrix with purpose of improving the compatibility between the components, and thereby Na-$MMTS_4$ and Fe-$MMTS_4$ were synthesized by treating Na-MMT and Fe-MMT with TSS, respectively. Silicone rubber/clay composites were prepared by compounding the clays with silicone rubber. Thermal stability and mechanical properties were evaluated as a function of the clay types and the surface modification.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.12 no.5
• /
• pp.2348-2365
• /
• 2018

QR codes as public patent are widely used to acquire the information in various fields. However, it faces security problem when delivering the privacy message by QR code. To overcome this weakness, we propose a secret hiding scheme by improving exploiting modification direction to protect the private message in QR code. The secret messages will be converted into octal digit stream and concealed to the cover QR code by overwriting the cover QR code public message bits. And the private messages can be faithfully decoded using the extraction function. In our secret hiding scheme, the QR code public message still can be fully decoded publicly from the marked QR codes via any standard QR Code reader, which helps to reduce attackers' curiosity. Experiments show that the proposed scheme is feasible, with high secret payload, high security protection level, and resistant to common image post-processing attacks.

• Proceedings of the Earthquake Engineering Society of Korea Conference
• /
• 2000.10a
• /
• pp.432-439
• /
• 2000

According as ground acceleration becomes to exceed gravity acceleration recently, design is impossible or economical efficiency is poor in existing seismic design method. So increase of seismic isolated bridges is currently in progress. However, because base isolation design method is developed in high seismic regions. it may not be compatible in Korea. Therefore, this research has objective to evaluate ductility of pier and response modification factor according to the ductility of pier in seismic isolated bridges and to adapt to seismic characteristics in Korea. For this purpose, nonlinear analysis is accomplished with so many time histories derived from spectral density function compatible with response spectrum described in the design code and base isolation system modeled linear system, bi-linear system, and friction system. Through application of the proposed method, we had result that it may be compatible that response modification factor for the seismic isolated bridges is smaller than half of that for the conventional bridges when natural period of structures exceeds proper level.

• ETRI Journal
• /
• v.32 no.6
• /
• pp.932-939
• /
• 2010

In this paper, a fast migration method is proposed. Our method executes local relocation on a model placement where an additional module is added to it for modification with a minimum number of displacements. This method is based on mean-field annealing (MFA), which produces a solution as reliable as a previously used method called simulated annealing. The proposed method requires substantially less time and hardware, and it is less sensitive to the initial and final temperatures. In addition, the solution runtime is mostly independent of the size and complexity of the input model placement. Our proposed MFA algorithm is optimized by enabling module rotation inside an energy function called permissible distances preservation energy. This, in turn, allows more options in moving the engaged modules. Finally, a three-phase cooling process governs the convergence of problem variables called neurons or spins.

• 제어로봇시스템학회:학술대회논문집
• /
• 1997.10a
• /
• pp.1367-1370
• /
• 1997

This paper presents a vision algorithm for finding the centers of steam generator tubes using the generalized symmetry transform, which is used for ECT(Eddy Current Test) of steam generator tubes in nuclear power plants. The geometrical properties of the image representing steam generator tubes shows that they have amost circular or somewhat elliptic appearances and each tube has strong symmetry about its center. So we apply the generalized symmetry transform to finding centers of steam geneator tubes. But applying the generalized symmetry transform itself without any modification gives difficulties in obtaining the exact centers of steam generator tubes. But applying the generalized symmetry transform itself without any modification gives difficulties in obtaining the exact centers of tubes due to the shadow effect generated by the local light installed inside steam generator. Therefore we make the generalized symmetry transform modified, which uses a modified phase weight function in getting the symmetry magnitude in order to overcome the misleading effect by the local light. The experimental results indicate that the proposed vision algorithm efficiently recongnizes centers of steam generator tubes.

• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 2003.10a
• /
• pp.9-19
• /
• 2003

Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. Many efforts have made characterization of GGTA1 in structure and function, improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTAl gene by Missouri group in 2002 To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. Of other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.