• 한국응용과학기술학회지
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• 제29권2호
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• pp.175-183
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• 2012

본 연구는 아토피 피부염을 갖는 질환동물에서 어성초를 비롯한 다양한 식물추출물을 이용하여 제조한 크림의 항아토피 피부염증에 대한 효능을 평가하였다. BALB/c 마우스 를 이용하여 아토피 피부염 유발 대조군 및 크림 처리군으로 완전 임의 배치하였다. 혈액 IgE와 히스타민 농도는 크림 처리군이 DNCB 유도 아토피 대조군에 비해서 유의하게 감소하였으며, 아토피 피부염이 심각한 상태의 마우스에서 정상적인 피부상태로 회복되었음을 관찰하였다. 본 결과는 아토피질환 모델 BALB/c 마우스에서 어성초 혼합 식물추출물의 투여가 혈액 IgE를 낮춤과 동시에 비만세포에서 생성되는 히스타민의 배출량을 억제함으로써 항아토피 효능을 갖는다는 점을 시사 해준다.

• Journal of Nutrition and Health
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• 제45권3호
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• pp.211-217
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• 2012

UV-irradiation is a major factor of photo-aged skin, by which pigmentation, wrinkles and laxity are increased. In addition, the epidermal barrier is disrupted, ultimately causing dryness in photo-aged skin. As an effort to search dietary sources for improving the dryness of UV irradiated skin, the dietary effect of red ginseng based functional foods on the epidermal level of ceramides, a major lipid maintaining epidermal barrier, was determined in this study. Albino hairless mice were fed either a control diet [group UV (UV-irradiated control)] or diets with 0.5% (group M0.5) or 1% (group M1.0) of red ginseng extracts mixed with Torilis fructus and Corni fructus (66.7% red ginseng) in parallel with UV irradiation for 5 wks. A normal control group (group C) was fed a control diet without UV irradiation for 5 wks. The epidermal level of ceramides in group UV was significantly lower than that in group C, in which ceramidase, an enzyme involved in ceramide degradation, was highly expressed. In group M0.5, the epidermal level of ceramide was significantly increased to the level even higher than in group C. In addition, protein expression of serine palmitoyl transferase (SPT), a key enzyme involved in de novo ceramide synthesis, was increased in group M0.5. However the epidermal levels of ceramides as well as of ceramidase protein expression in group M1.0 did not differ from those in group UV. In conclusion, we demonstrate that dietary supplementation of red-ginseng extracts mixed with Torilis fructus and Corni fructus at a level of 0.5% level in diet increased the epidermal level of ceramides coupled with the elevated expression of SPT protein.

• 동아시아식생활학회:학술대회논문집
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• 동아시아식생활학회 2003년도 춘계학술대회: 동아시아의 주식문화
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• pp.83-83
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• 2003

Electron donating ability by DPPH and nitrite scavenge were measured in ethanol extracts from raw, simple cooked and seasoned materials of Bibimbab(mixed rice), Korean traditional one-dish meal. Ethanol extracts of raw frugrantedible wild aster showed the highest electron donating abilities(80.28%) by DPPH. When its seasoned materials were compared with raw and simple cooked materials, it showed the highest electron donating abilities by DPPH.(중략)

• 한국자원식물학회지
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• 제23권5호
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• pp.423-429
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• 2010

본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당대사 관련 주요 효소인 glucokinase(GK)와 acetyl-CoA carboxylase(ACC)의 활성 및 $\alpha$-glucosidase 저해 활성을 측정하였다. 사용된 소재로는 지골피(Lycium chinense), 동충하초(Cordyceps militaris), 가시오가피(Acanthopanax senticosus)를 선정하였고, 이를 단독으로 사용하기 보다는 유효성분의 항당뇨 효능에 대한 상승효과를 알아보기 위하여 일정비율로 혼합하였다. 각 소재들을 일정 비율로 혼합한 모든 추출물에서 GK와 ACC의 활성이 증가하였으며, 가시오가피와 동충하초의 혼합 비율이 높아질수록 효소의 활성 증가율도 높아진 것으로 나타났다. 이는 지골피와 가시오가피, 동충하초의 상승작용 때문으로 사료된다. $\alpha$-glucosidase의 저해 활성은 JDG 5(1:6:6) 처리군에서 acarbose 보다 높은 억제율을 나타내었다. 결론적으로 지골피, 가시오가피, 동충하초의 혼합 비율이 1:6:6 일 때 당대사 관련 효소인 GK와 ACC 효소를 활성화시키고, $\alpha$-glucosidase의 활성을 저해하여 흡수된 당의 이용에 유리하게 작용함으로써 항당뇨 효과를 나타낼 수 있을 것으로 사료된다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.100-100
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• 2001

To develop an antimutagenic functional diet, the foods that have shown anticancer activity were mixed to make ready-to-eat powdered diets. The diets were prepared with various kinds of powdered cooked cereals, cooked legumes, oil seeds and sea tangles, and freeze-dried vegetables. The antimutagenic effects of methanol extracts from three mixed diets were investigated in the Ames test, SOS chromotest, and in vivo supravital staining micronucleus assay in the mice.(omitted)

• 한국식품과학회지
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• 제30권6호
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• pp.1404-1408
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• 1998

울금과 자초, 자초와 고삼, 고삼과 감초, 감초와 오미자 추출물 혼합액 1% 첨가가 배추김치의 보존성 향상에 미치는 영향을 $10^{\circ}C$에서 25일간 숙성시키면서 조사하였다. 김치의 pH와 산도는 숙성이 진행됨에 따라 급격히 감소, 증가하는 경향을 나타내었는데, 한약재 추출물을 혼합 첨가한 김치는 대조구에 비해 이의 속도가 매우 완만하게 나타났다. 특히 SG, GS 첨가구의 pH 감소속도와 산도 증가속도는 매우 느려 pH는 숙성 20일경 각각 4.19, 4.16을 나타내었고, 산도는 0.62, 0.64%를 나타내었다. 미생물 변화는 한약재 추출물 혼합 첨가구가 대조구에 비해 균 성장을 크게 억제시켰으며, 특히 GS, SG 첨가 김치는 적숙기인 숙성 15일째 대조구에 비해 총균수는 각각 1.3, 1.32 $log_{10}$ cycle, 유산균수는 1.24, 1.14 $log_{10}$ cycle 정도 억제되었다. 김치의 신맛은 김치 적숙기인 $10{\sim}15$일경 대조구에 비해 한약재 추출물 첨가구에서 약하게 나타났으며, 종합적인 맛은 한약재 고유의 향과 맛으로 인해 전반적으로 나쁘게 나타나 이를 보완하기 위한 연구가 수행되어야 할 것으로 보여진다.

• 대한한의학회지
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• 제29권1호
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• pp.85-94
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• 2008

Objectives : The purpose of this study was to investigate effects of extract from mixed culture with Tricholoma matsutake mycelium and Cordyceps militaris mycelium in oriental medicine and cereal midium (OCM) considered effective for the treatment of diabetes. Methods : We examined antioxidant activity and $\alpha$-glucosidase inhibit activity of OCM and orally administered OCM extracts to Streptozotocin-induced diabetic ratsat the dose of 100mg/kg, 200mg/kg and 300mg/kg body weight. Results : Total polyphenol content of extracts of OCM was 26.41 mg/g, DPPH radical scavenging activity was 32.24% and SOD-like activity was 31.43%. $\alpha$-glucosidase inhibit activity showed 85.3% of acarbose. Blood glucose levels of the OCM extracts-treated groups were lower than those of the diabetic control group (DC), total cholesterol and triglyceride contents were similar to the acarbose-treated group (PC) and ALT, AST were lower than the DC and PC groups. The 300 mg/kg body weight administered group (UM-300) were superior to other OCM-treated groups (UM-100, UM-200). Conclusions : These results showed extract from mixed culture with Tricholoma matsutake mycelium and Cordyceps militaris mycelium in oriental medicine and cereal midium has potentiality to beused in pharmaceutical products and medicinal food for preventive and treatment diabetes mellitus.

• 한국응용과학기술학회지
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• 제37권4호
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• pp.1020-1033
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• 2020

본 연구에서는 인체에 해가 없으며 항균작용 및 항산화 효과가 있는 오배자, 계피, 로즈마리 추출물을 이용하여 천연방부제 소재로서의 활용 가능성을 연구하였다. 실험식물의 추출방법으로는 계피의 경우 70% ethanol 추출, 오배자는 열수추출법, 로즈마리는 70% ethanol 추출과 열수추출법을 이용하였다. 실험 방법으로는 항균력, DPPH를 통한 항산화능, 화장품의 challenge test, 화장품 안정성 및 안전성 평가를 진행하였다. 시험 결과, 항균력 시험에서 오배자 추출물은 세균류, 계피 추출물은 진균류에서 항균력이 우수, 로즈마리 추출물의 경우 항균력이 미비해 제외, 복합 추출물(오배자·계피)의 경우는 1:1비율로 조합한 복합 추출물(complex C)에서 항균효과, 복합 추출물의 최소저해농도(MIC)와 최소살균농도(MBC)에서는 5종의 실험균주에서 억제효과와 살균효과, 천연 단일 추출물과 복합 추출물(complex C)에서는 항산화능이 확인 되었다. 또한 합성 방부제 대신 complex C 10%를 화장품에 적용한 시험에서 제형의 안정성 및 안전성이 확인되었다. 따라서 본 연구를 통해 천연방부제 원료로서의 발전 가능성을 확인할 수 있었다.

• Mycobiology
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• 제28권4호
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• pp.185-189
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• 2000

The antifungal activity of methanolic extracts of Centella asiatica and Andrographis paniculata leaves was observed against fourteen fungi, viz., Alternaria alternata, A. brassicae, A. brassicicola, A. solani, A. tenuissima, Cercospora blumae, Curvularia lunata, C. penniseti, and Drechslera monoceras, D. oryzae, D. turitica, Fusarium albizziae and F. udum. Different concentrations of the methanolic extract (1000, 2000, 3000, 5000, 7000, 10000ppm) were used. The effect of mixed leaf extract (1500 ppm of C. asiatica + 1500 ppm of A. paniculata) and its 1:2 ad 1:4 dilutions were also studied. The individual extracts of both the plants showed significant inhibitory effect on spore germination of all the fungi tested. F. udum, F. albizzae, D. oryzae, D. turtica, and D. monoceras were particularly sensitive to these extracts. In general, the extract of C. asiatica showed a higher inhibitory effect in all concentrations against all the fungi as compared to A. paniculata, except for A. brassicae A. solani, D. oryzae, D. penniseti and Curvularia sp. The inhibitory effect of extracts increased when they were used in combination with or without dilutions against A. brassicicola, A. solani A. brassicae, A. alternata, A. tenussima, C. blumae, C. lunata, C. penniseti and Curvularia species. Higher efficacy of active ingredient of these extracts under field condition is envisaged against plant pathogens.

• 한국지역사회생활과학회지
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• 제21권1호
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• pp.139-148
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• 2010

This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.