• Title/Summary/Keyword: Mixed Compound

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Isolation and In Vitro Antimicrobial Activity of Low Molecular Phenolic Compounds from Burkholderia sp. MP-1 (Brukholderia sp. MP-1 에서의 페놀화합물의 분리와 항균활성의 측정)

  • Mao, Sopheareth;Jin, Rong-De;Lee, Seung-Je;Kim, Yong-Woong;Kim, In-Seon;Shim, Jae-Han;Park, Ro-Dong;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.195-203
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    • 2006
  • An antagonistic strain, Burkholderia MP-1, showed antimicrobial activity against various filamentous plant pathogenic fungi, yeasts and food borne bacteria (Gram-positive and Gram-negative). The nucleotide sequence of the 16S rRNA gene (1491 pb) of strain MP-1 exhibited close similarity (99-100%) with other Burkholderia 16S rRNA genes. Isolation of the antibiotic substances from culture broth was fractionated by ethyl acetate (EtOAc) solvent and EtOAc-soluble acidic fraction. The antibiotic substances were purified through a silica gel, Sephadex LH-20, ODS column chromatography, and high performance liquid chromatography, respectively. Four active substances were identified as phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid and 4-hydroxyphenylacetate methyl ester by gas chromatographic-mass spectrum analysis. The minimum inhibition of concentration (MIC) of each active compound inhibited the growth of the microorganisms tested at 250 to $2500{\mu}g\;ml^{-1}$. The antimicrobial activity of crude acidic fraction at 1 mg of dry weight per 6 mm paper disc was more effective than authentic standard mixture (four active substances were mixed with the same ratio as acidic fraction) over a wide range of bacterial test.

Study on the Fire Cause Analysis for Explosives Waste by Thermal Analysis Experiment (열분석 실험에 의한 화약류 폐기물의 화재원인분석에 관한 연구)

  • Koh, Jae-Sun
    • Journal of the Society of Disaster Information
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    • v.14 no.1
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    • pp.89-100
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    • 2018
  • when the explosive wastes to be treated as designated wastes are brought into the wastes treatment plant by mistake and lead to an explosion in the wastes disposal process, many people and property damage are involved. Waste should be treated properly. As mentioned in this paper, ignition reac- tion tests of ignitable re-burning of explosives packing material waste (solid butadiene) confirmed that ignition was easily occurred, and that even small ignition sources were easily ignited and burned quickly and explosively. In particular, when explosives are loaded into incineration wastes in large quantities and mixed with organic compound wastes, such as fire and explosion accidents caused by explosives packing materials at waste disposal sites, flammable and oxidative gases are generated due to mutual oxidation and pyrolysis It is confirmed that there is a possibility that ignition sources such as spark ignite and instantaneously lead to explosion. It is hoped that this study will be a small reference for on - site detection in the field of fire, and it is expected that the fire - fighting agency will be recognized as a fire investigation agency and will contribute to the improvement of the credibility.

Extraction and Characterization of an Anti-hyperglycemic α-Glucosidase Inhibitor from Edible Mushroom, Pleurotus cornucopiae (식용버섯인 노랑느타리버섯으로부터 혈당상승억제성 α-glucosidase 저해제의 추출 및 특성)

  • Bae, Sang-Min;Han, Sang-Min;Lee, Yun-Hae;Jung, Youn-Kyung;Ji, Jeong-Hyun;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
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    • v.44 no.2
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    • pp.124-129
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    • 2016
  • The extraction and purification of the anti-hyperglycemic α-glucosidase inhibitor from an edible mushroom, Pleurotus cornucopiae, were investigated. The inhibitor was maximally extracted when the P. cornucopiae fruiting body was treated with distilled water at 30℃ for 12 h. Purification was achieved using Sephadex G-100 and G-50 filtration chromatography, pepsin hydrolysis, and reverse-phase HPLC. The compound’s solid yield and inhibitory activity were 12.2% and 9.10 mg/ml of IC50, respectively. The purified inhibitor contained two hexapeptides with Thr-Ile-Ala-Phe-Ile-Asp (A) and Tyr-Tyr-Ala-Ile-Gly-Asp (B) sequences and molecular weights of 678.79 Da (A) and 643.7 Da (B). The purified inhibitor showed a mixed inhibition pattern to α-glucosidase and a dose-dependent anti-hyperglycemic effect in a streptozotocininduced diabetic Sprague-Dawley rat model, exhibited by decreased blood glucose levels at doses of 50 and 300 mg/kg.

Mixed Noise Reduction Filters for CR Images (CR X선 영상의 복합잡음 감소에 관한 연구)

  • Min, Jung-Whan;Jeong, Hea-Won;Kim, Jung-Min
    • Journal of radiological science and technology
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    • v.30 no.1
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    • pp.1-6
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    • 2007
  • This study is to decrease compound noise in x-ray films. This study compared Signal to noise ratio(SNR), Peak signal to noise ratio(PSNR), Mean square error(MSE) to surface of the earth. In addition, we evaluated noise elimination effect according to the kernel size of Median filter. This experiments show that some filters are useful by finding image that is near in circle image comparing circle picture with each processed picture. In noise power value, when cutoff frequency was compared with other filters of cutoff frequency. Cutoff frequency of $2/3\pi{\sim}3/4\pi$ is good and it shows good SNR and PSNR. Therefore, it can display high filter effect. As Median Filter's Kernel size grows SNR value gets bigger, which shows better filter effect. Most pictures are distorted after filter application in medical treatment image. It is important to keep spatial resolution in most medical images. Visual estimation as well as quantitative indicators should be necessary for a better image.

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Field Applicability Evaluation Using Effective Microorganism Brewing Cycle for Contaminated Soil in Water Retention Basin (복합발효미생물을 이용한 하천유수지 오염토의 현장적용성 평가)

  • Shin, Eunchul;Jung, Minkyo;Kim, Kyeongsig;Kang, Jeongku
    • Journal of the Korean GEO-environmental Society
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    • v.17 no.11
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    • pp.35-43
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    • 2016
  • In this study, by using a Effective Microorganisms Brewing Cycle, it confirmed the purification effect of pollutants that are adsorbed on the basins stench removal and retarding soil. On the basis of on-site application test, a soil decontamination system will be suggested. Using a Effective Microorganisms Brewing Cycle, the odor concentration is reduced 2.5 times than that of natural purification treatment method. It was measured and found that the quality of the pore water discharged from the soil is improved. In addition, it was found that a composite of copper and lead with the fermentation microorganisms adsorbed on soil particles from the surface of the stirred experiments lagoon mixed soil is reduced to 65% and 66%, respectively, The TPH organic component was confirmed that the reduction effect of 85%. Restoration of reservoir contaminated soils using the effective microorganism brewing cycle needs to be more developed and implemented as a long-term purification system. This study may be a good reference of developing more complete microorganism brewing system which will efficiently reduce the odor and soil contamination based on optimal stirring and mixing ratio of the compound solutions and contaminated soils in reservoir.

Quality Characteristics of Makgeolli Added with Kiwifruit (Actinidia deliciosa) (키위를 첨가한 막걸리의 품질특성)

  • Kim, Eunkyung;Chang, Yoon Hyuk;Ko, Jae Youn;Jeong, Yoonhwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.11
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    • pp.1821-1828
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    • 2013
  • This study was conducted to investigate the physicochemical and microbial properties of Makgeolli supplemented with kiwifruit (Actinidia deliciosa). Four hundred grams of kiwifruit were added to 3.1 L of distilled water, followed by 2.0 kg of rice, 40.0 g of Nuruk, and 14.0 g of yeast. The mixed rice solution was then fermented at $28^{\circ}C$ for 6 days to prepare the kiwifruit Makgeolli. The pH values of the kiwifruit Makgeolli decreased from 5.31 to 4.37, but the total acidity values increased from 0.05 to 0.34% during fermentation. The total viable cells ($3.18{\times}10^7$ and $2.88{\times}10^7$, respectively), lactic acid bacteria ($1.51{\times}10^6$ and $1.50{\times}10^6$, respectively), and yeast counts ($1.96{\times}10^7$ and $1.90{\times}10^7$, respectively) of the kiwifruit Makgeolli and control were similar throughout the fermentation process. Glucose was the major free sugar in the control and kiwifruit Makgeolli and significantly decreased during fermentation. Succinic acid was the highest organic acid in both the control (24.6 mg/mL) and kiwifruit Makgeolli (26.3 mg/mL). In a volatile compound analysis, 3-methyl-1-butanol, 2-methyl-1-propanol and ethyl acetate were the major volatile compounds in the kiwifruit Makgeolli.

Weathering Impact for Rock Properties and Material Characteristics of Concretes Used Stone Pagoda of the Mireuksaji Temple Site, Iksan, Korea (익산 미륵사지 석탑에 사용된 콘크리트의 재료학적 특성과 석재의 풍화에 미치는 영향)

  • Lee Dong-Sik;Lee Chan-Hee;Kim Ji-Young
    • Economic and Environmental Geology
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    • v.39 no.3 s.178
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    • pp.285-299
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    • 2006
  • The Mireuksaji stone pagoda in Iksan is the largest stone pagoda existing in Eastern Asia. It was assumed that originally it had been established in the shape of nine-storied pagoda but as time went by only six-storied pagoda remained partially due to collapsing, repair and reconstruction. According to the reference, we can't make sure when its modification happened. The form that the pagoda is having now, was modified with concrete by the Japanese during the 1910s. The materials mixed in concrete were mixture of Portland cement, all sorts of stone, sand, and a little bit of new building stone, additive and compound. And also these materials were applied to cultural assets without any experiment at the time of 1910s as maintenance, which are still used recently. To prevent the change of its shape, the west side, south side and the north side which is partially destructed was rebuilt and reinforced with concrete and some of the deformed parts were also filled. The amounts of concrete used were about 200 tons. Such method had prevented the pagoda from destructing, however, by choosing a wrong repair method, its surface of the stone has secondary contaminants and precipitation caused by concrete. This kind of contamination speeds up the weathering which accelerates the aging mechanism of the stone to make it even harder to revive the absence of historical nature. Therefore, we are to find the best cleaning method to remove the secondary hazard contaminants.

Dispersion Characteristics of Carbon Black Particles in a High Viscous Simulated Solution (고점성 모사용액 내 Carbon Black 입자의 분산특성)

  • Jeong, Kyung-Chai;Eom, Sung-Ho;Kim, Yeon-Ku;Cho, Moon Sung
    • Applied Chemistry for Engineering
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    • v.24 no.2
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    • pp.165-170
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    • 2013
  • An external gelation method in place of an internal gelation method applied to the fabrication process of an intermediated compound of Uranium Oxy-Carbide (UCO) kernel spheres for Very High Temperature Reactor (VHTR) fuel preparation is under development in Korea. For the preliminary experiments of the UCO kernel sphere preparation using an external gelation method, the carbon black dispersion experiments were carried out using a simulated broth solution. From the selection experiments of various kinds of carbon black through dispersion experiments in a viscous metal salt solution, Cabot G carbon black was selected owing to its dispersion stability, and the homogeneous dispersing state of carbon black particles in our system. For the effective dispersion of nano-size aggregated carbon black particles in a high viscous liquid, the carbon black particles in a metal salt solution were first de-aggregated with ultrasonic force. The mixed solution was then dispersed secondly by the use of the extremely high-speed agitation with a mechanical mixer of 6000 rpm after feeding the Poly Vinyl Alcohol (PVA) in the solution. This results in the broth solution with good stability and homogeneity alongside no further changes in physical properties.

Refractive Error in Middle and High School Pupils (중고등학생의 안굴절 상태조사 및 연구)

  • Pyo, Kyoung-Min;Kim, Woong-Tea
    • Journal of Korean Ophthalmic Optics Society
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    • v.1 no.1
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    • pp.119-125
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    • 1996
  • From 1995. 3. 20 to 1995. 4. 10 refractive errors were surveyed on the 1,197 middle and high school pupils in Dong-Du-Cuon area. On the basis of the power of the old glasses or newly examined power, we surveyed the distribution of the refractive errors according to their types, ages, heights, weights and differences between both eyes. Among 2,394 eyes examined, 1,125eyes(47%) were emmetropia, 967 eyes(40.4%) were myopic, and 302eyes(12.6%) were hyperopic. As to the distribution of refractive errors, simple myopia(42.9%) was most common, and mixed astigmatism and compound hyperopic astigmatism were below 1%, Among the types of astigmatism, 62% were with the rule, 23% were against the rule and 15% were oblique, Differences between both eyes, the left eye showed more myopic than those of right eye. The distribution of myopia according to their ages, 27.7 at age 11, 38.5% at age 12, 35.5% at age 41.5% at age 14, 37.7% at age 15, 48.3% at age 16 and 46.8% at age 17., there was a significant increase in the prevalence of the myopia but that or hyperopia decreases with increases of age.

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Development of Meat-like Flavor by Maillard Reaction with Addition of Natural Flavoring Materials (천연 조미향상물질의 첨가에 의한 Maillard 반응에서 Meat-like Flavor의 개발)

  • Ko, Soon-Nam;Nam, Hee-Sop;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.839-846
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    • 1997
  • Addition of three natural flavoring materials, hydrolyzed vegetable protein (HVP), hydrolyzed animal protein (HAP) and yeast extract (YE), into 0.2 M cystine-0.1 M lactose-0.1 M maltose solution (control) was studied for development of meat-like flavor by Maillard reaction. The HVP, HAP and YE were added individually at various concentrations and were mixed at selected concentration in order to compare their effects. The absorbance, color, sensory characteristics and volatile compounds of the solutions after the reaction at $100^{\circ}C$ for 8 hr were measured. The results showed that the absorbances of reaction solution at 420 nm and 278 nm were increased as reaction time and the concentration of the natural flavoring material increased. Also ‘L’ values of reaction solutions added with HVP, HAP or YE decreased while the ‘b’ value increased slightly. From the results of sensory evaluation 1.16% HVP, 0.94% HAP, 1.48% YE or 1.16% HVP + 0.94% HAP were selected as the appropriate substrates for the meat-like flavor development. The volatile compounds identified by GC/MS for the control and those added with 1.16% HVP or 1.16% HVP+0.94% HAP were 1 hydrocarbons, 9 aldehydes, 5 ketones, 1 ester, 5 alcohols, 2 aromatics(benzene), 2 furans, 1 sulfur compound.

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