Objectives: To determine the relationship between 2D:4D ratio and Minnesota Multiphasic Personality Inventory-2 Questionnaire (MMPI-2 scales). Methods: The 2D:4D ratio in 101 Korean medical students was measured. MMPI-2 test was performed. Results: In the unisex group, there were significantly positive correlations between the 2D:4D ratio and MMPI-2 scales of Hs, D, Pt, Sc, Si, RC1, RC2, RC8, INTR, ANX, OBS, HEA, BIZ, LSE, WRK, TRT, A, R, Mt, PK, and AAS while there were significantly negative correlations between the 2D:4D ratio and MMPI-2 scales of Es and Do. In the male group, there were significantly positive correlations between the 2D:4D ratio and MMPI-2 scales of Hs, RC1, RC8, HEA, and AAS while there were significantly negative correlations between the 2D:4D ratio and MMPI-2 scale of AGGR. In the female group, the 2D:4D ratio showed significantly positive correlations with MMPI-2 scales of Pt, Sc, Si, RC1, RC2, RC6, INTR, DEP, BIZ, LSE, WRK, A, Mt, PK, and Ho while it showed significantly negative correlations with MMPI-2 scale of Do. Conclusions: MMPI-2 scales are correlated with the 2D: 4D ratio. Therefore, 2D: 4D ratio could be used as an indicator to reflect multiphasic personality of individuals.