• Food Science and Biotechnology
• /
• v.18 no.4
• /
• pp.823-832
• /
• 2009

In recent years, special concerns have been raised about the safety assessment of foods and food ingredients derived from genetically modified organisms (GMOs). A growing number of countries establish regulations and laws for GMOs in order to allow consumers an informed choice. In this case, a lot of methods have been developed for the detection of GMOs. However, the reproducibility among methods and laboratories is still a problem. Consequently, it is still in great demand for more effective methods. In comparison with the gel electrophoresis, the capillary electrophoresis (CE) technology has some unique advantages, such as high resolution efficiency and less time consumption. Therefore, some CE-based methods have been developed for the detection of GMOs in recent years. All kinds of CE detection methods, such as ultraviolet (UV), laser induced fluorescence (LIF), and chemiluminescence (CL) detection, have been used for GMOs detection. Microchip capillary electrophoresis (MCE) methods have also been used for GMOs detection and they have shown some unique advantages.

• Journal of the Korean Society for Nondestructive Testing
• /
• v.22 no.6
• /
• pp.599-608
• /
• 2002

The mechanical properties of mullite $(3Al_2O_3\;2SiO_2)$ environmental barrier coatings are determined using a laser-based ultrasonic system. The waveforms generated by a laser source in mullite coatings in the $1-20{\mu}m$ thickness range are evaluated theoretically using the integral transform technique. It is shown that the laser source generated the two lowest order SAW modes in these systems. Experimental waveforms are generated using a 600ps pulsed Nd:YAG microchip laser and detected using a stabilized Michelson interferometer. The dispersion curves for the generated modes are extracted from the experimental data and the mechanical properties of the coatings are obtained by minimizing the error between the measured and calculated velocity values. The waveforms generated in mullite coatings agree well with theory and laser-based ultrasonics is shown to provide an effective tool for the nondestructive evaluation of ceramic coatings.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2008.10a
• /
• pp.1335-1336
• /
• 2008

A compact diode-pumped microchip(DPM) green light source which consist of pump LD, $Nd:YVO_4$, KTP and a built-in thermoelectric element has been fabricated for laser projection display. The volume of the source is as small as $0.7\;cm^3$ and we obtained the output power of 25mW with the average electric power consumption in pump LD of 304mW which correspond to the electrical-to-optical efficiency of 7.8%. Another approach using quasi phase matching(QPM) technique has been attempted. We obtained the output power of 90mW of green beam with 350mW pumping of a $Nd:YVO_4$ laser with 20-kHz rep. rate and 10-ns pulse duration. Detailed characteristics and issues of the DPM and QPM green light sources will be addressed.

• Proceedings of the IEEK Conference
• /
• 2001.09a
• /
• pp.777-783
• /
• 2001

This paper is mainly concerned with the state of the practical developments of a constants PWM bridge type resonants DC-DC suitable converter for Nd:YAG Laser with a Microprocessor. (PIC16C54 & 8051) The use of IGBT power supply with feedback control of flashLamp currents imparts a advantages to Nd:YAG Laser for materials processing. these include the alility to tailor the pulseshape and modify pulse parameters on a pulse- by pulse basis. And Correct choice of pulseshape can enhance the repeatability of the process. as higher power IGBT became available, act ive pulseforming power supplies will find greater user in deep hole drilling machine By Using certain control tecniques, utililized in designing Pic16c54 from Microchip technology and Intel 8051, also Mornitoring from Microsoft Visual Basic 5, And it allowed us to designed and fabricate ahigh repel it ion rate and high power(HRHP) pulsed Nd:YAG laser system, As a result of that, the current pulsewidth could be contort led 200s to 350s(step 50s) , and the pulse repetition rate could be adjusted 500pps to 1150pps. In addition, in the case of one laser head consisting of a Nd:YAG laser rod and two flashlamps , the maximum laser output of 240w was produced at the condition of 350s and 1150pps, and that of about 480w was generated at the same condition when two laser heads were arranged in cascade.

• Proceedings of the Optical Society of Korea Conference
• /
• 2002.07a
• /
• pp.72-73
• /
• 2002

매우 작은 이득 매질 안에 높은 농도의 레이저 이온을 도핑하여 공진기의 길이를 수 3m 이내로 대폭 축소하였으면서도 기존의 고체 레이저보다 효율적이며, 소형화된 마이크로 칩 레이저에 관한 연구는 최근 여러 연구진에 의해 활발하게 진행되고 있다. 기존의 YAG는 열 전달 계수가 크며, 역학적, 광학적, 화학적 성질이 모두 매우 우수한 레이저 매질로 평가되어왔으나 대표적인 레이저 이온인 Nd이온과 비교하여 Yttrium이온의 크기가 커서 도핑 농도를 높이는데 한계를 가지고 있다. (중략)

• Korean Journal of Optics and Photonics
• /
• v.11 no.4
• /
• pp.294-298
• /
• 2000

The output characteristics of intracavity frequency doubling of laser-diode end-pumped Nd:S-VAP laser were investigated. Nd:S-VAP is suitable for a microchip laser medium, which has a low threshold property because of a very high value of the stimulated emission cross-section and lifetime product. The threshold energy measured was 81 J.ll. The second harmonic output energy measured was $126\mu\textrm{J}$at a pump energy of $2\mu\textrm{J}$. We described for intracavity frequency doubling by using theoretical calculations. Q-switched second harmonic energy measured was $15\mu\textrm{J}$per pulse with a pulse-width of 26 ns. at a pump energy of 2 mJ and an $M^2$ of 1.47 represented a good beam quality. ality.

• Proceedings of the KIEE Conference
• /
• 2006.07c
• /
• pp.1629-1630
• /
• 2006

This paper describes a micro total analysis system ($\mu$ TAS) for detecting and digesting the target protein which includes a bead based temperature controllable microchip and computer based controllers for temperature and valve actuation. We firstly combined the temperature control function with a bead based microchip and realized the on-chip sequential reactions using two kinds of beads. The PEG-grafted bead, on which RNA aptamer was immobilized, was used for capturing and releasing the target protein. The target protein can be chosen by the type of RNA aptamer. In this paper, we used the RNA aptamer of HCV replicase. The trypsin coated bead was used for digesting the released protein prior to the matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI TOF MS). Heat is applied for release of the captured protein binding on the bead, thermal denaturation and trypsin digestion. PDMS microchannel and PDMS micro pneumatic valves were also combined for the small volume liquid handling. The entire procedures for the detection and the digestion of the target protein were successfully carried out on a microchip without any other chemical treatment or off-chip handling using $20\;{\mu}l$ protein mixture within 20 min. We could acquire six matched peaks (7% sequence coverage) of HCV replicase.

• Proceedings of the Optical Society of Korea Conference
• /
• 2003.02a
• /
• pp.234-235
• /
• 2003

All solid-state UV lasers provide efficient, clean and semipermanent light sources for various applications, such as eye surgery, microchip lithography. $CsLiB_{6}O_{10}$ (CLBO) is one of the most suitable crystals for UV generation because of its small walk-off, large effective nonlinear susceptibility in UV region and high damage threshold. We produced fourth (266 nm) and fifth (213 nm) harmonic generation of an Nd：YAG laser (1064 nm) with $LiB_{3}O_{5}$ as a second harmonic generation medium and CLBO as a fourth harmonic and fifth harmonic mediums. (omitted)

• Journal of Welding and Joining
• /
• v.26 no.3
• /
• pp.30-36
• /
• 2008

A flip-chip bonding system using IR laser with a wavelength of 1064 nm was developed and associated process parameters were analyzed using Taguchi methods. An infrared laser beam is designed to transmit through a silicon chip and used for transferring laser energy directly to micro-bumps. This process has several advantages: minimized heat affect zone, fast bonding and good reliability in the microchip bonding interface. Approximately 50 % of the irradiated energy can be directly used for bonding the solder bumps with a few seconds of bonding time. A flip-chip with 120 solder bumps was used for this experiment and the composition of the solder bump was Sn3.0Ag0.5Cu. The main processing parameters for IR laser flip-chip bonding were laser power, scanning speed, a spot size and UBM thickness. Taguchi methods were applied for optimizing these four main processing parameters. The optimized bump shape and its shear force were modeled and the experimental results were compared with them. The analysis results indicate that the bump shape and its shear force are dominantly influenced by laser power and scanning speed over a laser spot size. In addition, various effects of processing parameters for IR laser flip-chip bonding are presented and discussed.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.1 no.4
• /
• pp.239-247
• /
• 2001

The Fluorescence-Activated Cell Sorter (FACS) is a well-established instrument used for identifying, enumerating, classifying and sorting cells by their physical and optical characteristics. For a miniaturized FACS device, a disposable plastic microchip has been developed which has a hydrodynamic focusing chamber using soft lithography. As the characteristics of the spatially confined sample stream have an effect on sample throughput, detection efficiency, and the accuracy of cell sorting, systematic fluid dynamic studies are required. Flow visualization is conducted with a laser scanning confocal microscopy (LSCM), and three-dimensional flow structure of the focused sample stream is reconstructed from 2D slices acquired at $1\mutextrm{m}$ intervals in depth. It was observed that the flow structure in the focusing chamber is skewed by unsymmetrical velocity profile arising from trapezoidal cross section of the microchannel. For a quantitative analysis of a microscopic flow structure, Confocal Micro-PIV system has been developed to evaluate the accelerated flow field in the focusing chamber. This study proposes a method which defines the depth of the measurement volume using a detection pinhole. The trajectories of red blood cells (RBCs) and their interactions with surrounding flow field in the squeezed sample stream are evaluated to find optimal shape of the focusing chamber and fluid manipulation scheme for stable cell transporting, efficient detection, and sorting