• Title/Summary/Keyword: Mass activity

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High-Throughput Active Compound Discovery using Correlations between Activity and Mass Profiles

  • Park, Kyu-Hwan;Yoon, Kyo-Joong;Kwon, Kyung-Hoon;Kim, Hyun-Sik
    • Mass Spectrometry Letters
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    • v.1 no.1
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    • pp.13-16
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    • 2010
  • The active components in a plant extract can be represented as mass profiles. We introduce here a new, multi-compound discovery method known as Scaling of Correlations between Activity and Mass Profiles (SCAMP). In this method, a correlation coefficient is used to quantify similarities between the extract activity and mass profiles. The method was evaluated by first measuring the anti-oxidation activity of eleven fractions of an Astragali Radix extract using DPPH assays. Next, 15 T Fouriertransform ion cyclotron resonance (FT-ICR) MS was employed to generate mass profiles of the eleven fractions. A comparison of correlation coefficients indicated two compounds at m/z 285.076 and 286.076 that were strong antioxidants. Principal component analyses of these profiles yielded the same result. FT-ICR MS, which offers a mass resolving power of 500,000, was used to discern isotopic fine structures and indicated that the molecular formula corresponding to the peak at m/z 285.076 was $C_{16}H_{13}O_5$. SCAMP in combination with high-resolution MS can be applied to any type of mixture to study pharmacological activity and is a powerful tool for active compound discovery in plant extract studies.

Attenuated Sympathetic Activity and Its Relation to Obesity in MSG Injected and Sympathectomized Rats

  • Park, So-Young;Kim, Yong-Woon;Dan, Jin-Myoung;Kim, Jong-Yeon
    • The Korean Journal of Physiology and Pharmacology
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    • v.11 no.4
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    • pp.155-161
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    • 2007
  • In order to characterize the role of sympathetic activity in obesity, we repeatedly assessed sympathetic activity via power spectral analyses of heart rate variability in the same subjects at 7, 11, 25, and 60 weeks, using monosodium glutamate (MSG)-induced obese and control rats. The effects of lower sympathetic activity on obesity were also evaluated. Fat mass in MSG rats was already higher at 7 weeks, but the sympathetic activity did not differ between 7 and 25 weeks. Between 25 and 60 weeks, the increase in fat mass, food efficiency, and body weight gain was higher in MSG rats. The increase in sympathetic activity between 25 and 60 weeks and sympathetic activity at 60 weeks were lower in MSG rats. Fat mass at 60 weeks was inversely correlated with changes in sympathetic activity between 25 and 60 weeks. Reduced plasma epinephrine levels by bilateral adrenal demedullation induced increase of fat mass. In, an attenuated increase of sympathetic activity with age may partly be responsible for aggravated obesity in MSG rats. Additionally, reduced sympathetic activity per se induced obesity in rats. These results suggest that lower sympathetic activity contributes to obesity in rats.

Disruption of Established Bacterial and Fungal Biofilms by a Blend of Enzymes and Botanical Extracts

  • Gitte S. Jensen;Dina Cruickshank;Debby E. Hamilton
    • Journal of Microbiology and Biotechnology
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    • v.33 no.6
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    • pp.715-723
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    • 2023
  • Microbial biofilms are resilient, immune-evasive, often antibiotic-resistant health challenges, and increasingly the target for research into novel therapeutic strategies. We evaluated the effects of a nutraceutical enzyme and botanical blend (NEBB) on established biofilm. Five microbial strains with known implications in chronic human illnesses were tested: Candida albicans, Staphylococcus aureus, Staphylococcus simulans (coagulase-negative, penicillin-resistant), Borrelia burgdorferi, and Pseudomonas aeruginosa. The strains were allowed to form biofilm in vitro. Biofilm cultures were treated with NEBB containing enzymes targeted at lipids, proteins, and sugars, also containing the mucolytic compound N-acetyl cysteine, along with antimicrobial extracts from cranberry, berberine, rosemary, and peppermint. The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. For B. burgdorferi, we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. For P. aeruginosa, low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. The results suggest that targeted nutraceutical support may help disrupt biofilm communities, offering new facets for integrative combinational treatment strategies.

Differences in Chronic Diseases and Physical Activity of Elderly Women According to Body Mass Index (재가여성노인의 비만도에 따른 만성질병과 신체활동정도의 차이)

  • Jin, Eun-Hee
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.17 no.2
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    • pp.120-126
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    • 2010
  • Purpose: The study sought to identify the differences in chronic diseases and physical activity in elderly women by BMI. Method: The subjects of this study were 644 elderly women 60-80-years-of-age living at home. The research instruments were physical activity levels and chronic diseases. Subjects were given a self-report questionnaire. Data were analyzed using the SPSS win program. Result: Hypertension, prevalence of diabetes mellitus and hyperlipemia were significantly different in the subjects according to body mass index, being higher in obese subjects than in non-obese subjects. Physical activity in each of the body mass index groups did not differ significantly. Conclusion: Obesity increases the risk of chronic diseases. This knowledge could help elderly women control their weight, reduce chronic diseases, and ultimately, gain better health.

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Relationship between Exercising Activity, Muscle Mass, Muscular Strength and Cognitive Function in the Elderly (노인의 운동참여, 근육량, 근력 및 인지기능과의 관련성)

  • Choi, Jae-won;Yoo, Ha-Na;Lee, Hyun-Woo;Kang, Sung-Goo;Jung, Hye-Yeon
    • Journal of the Korea Convergence Society
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    • v.9 no.5
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    • pp.219-229
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    • 2018
  • The purpose of this study was to investigate the relationship among Exercising Activity, muscle mass, Muscular strength and cognitive function of 274 elderly people aged 65 years and older living in Seoul and Gyeonggi-do As for the data collection methods, were measured the Exercising Activity, muscle mass and muscular strength and the Korean version of Mini Mental State Examination(MMSE-K) and digit span test were measured. The results are as follows First, there was a statistically significant correlation between Exercising activity, muscle mass, muscular strength, and cognitive function of the elderly. Second, Exercising activity of the elderly showed a positive (+) influence on muscle mass, muscular strength, and cognitive function. Third, the muscle mass of the elderly showed a statistically significant (+) influence on muscular strength and cognitive function. Fourth, the strength of the elderly showed a positive (+) influence on cognitive function.

Prediction Model for Reduced Bone mass in Women using Individual Characteristics & Life Style Factors (여성의 개인적 특성과 생활양식요인을 이용한 골량감소 예측모형)

  • Lee, Eun-Nam;Lee, Eun-Ok
    • Journal of muscle and joint health
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    • v.5 no.1
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    • pp.83-109
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    • 1998
  • This study was carried out to identify the Important modifiable risk factors for reduced bone mass and to construct prediction model which can classify women with either low or high bone mass. Through the literature review, individual characteristics such as age, body weight, height, education level, family history, age of menarche, postmenopausal period, gravity, parity, menopausal status, and breast feeding period were identified and factors of life style such as past milk consumption, past physical activity, present daily activity, present calcium intake, alcohol intake, cigarette smoking, coffee consumption were identified as influencing factors of reduced bone mass in women. Four hundred and eighty women aged between 28 and 76 who had given measurement bone mineral density by dual energy x-ray absortiometry in lumbar vertebrae and femur from July to October, 1997 at 4 general hospitals in Seoul and Pusan were selected for this study. Women were excluded if they had a history of any chronic illness such as rheumatoid arthritis, diabetes mellitus, hyperthroidism, & gastrointestinal disorder and any medication such as calcium supplements, calcitonin, estrogen, thyroxine, antacids, & corticosteroids known affect bone. As a result of these exclusion criteria, four hundred and seventeen women were used for analysis. Multiple logistic regression model was developed for estimating the likelihood of the presence or absence of reduced bone mass. A SAS procedure was used to estimate risk factor coefficient. The results are as follows For lumbar spine, the variables significant were age, body weight, menopause status, daily activity, past milk consumption, and past physical activity(p<0.01), while for femoral Ward's triangle, age, body weight, level of education, past milk consumption, past physical activity(p<0.001). Past physical activity, present daily activity and past milk consumption are the most powerful modifiable predictors in vertebrae and femur among the predictors. When the model performance was evaluated by comparing the observed outcome with predicted outcome, the model correctly identified 74.1% of persons with reduced bone mass and 84.5% of persons with normal bone mass in the lumbar vertebrae and 82.9% of persons with reduced bone mass and 75.0% of persons with normal bone mass in the femoral Ward's triangle. On the basis of these results, a number of recommendations for the management of reduced bone mass may be made : First, those woman who are classified as high risk group of the reduced bone mass in the prediction model should examine the bone mineral density to further examine the usefulness of this model. Second, the optimal amount of milk consumption and a regular weight bearing exercise in childhood, adolescence, and early adult should be ensured.

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A Longitudinal Study on Seasonal Variations of Physical Activity and Body Composition of Rural Women (농촌여성들의 계절별 활동량과 체성분 차이에 관한 종단 연구)

  • 임화재
    • Journal of Nutrition and Health
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    • v.28 no.9
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    • pp.893-903
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    • 1995
  • This study was attempted to estimate seasonal variations of physical activity level, energy balance & body composition of 38 rural women, aged 31 to 67yrs in three seasons ; farming season(June), harvesting season(October), nonfarming season(February) Energy intake, energy expenditure and body composition were assessed using questionnaire, daily activity diary & bioelectrical impedance. The type of activities & the period of the spent on each activity were changed significantly with the seasons. Daily mean duration(min) of farming activity was significantly higher in June & October than in February(p<0.005) & daily mean energy expenditure for farming activity was significantly high in June & low inFebruary (p<0.005). Daily mean energy expenditure was 2892.9㎉ in June, 2487.4㎉ in October and 2130.9㎉ in February and changed significantly in three seasons(p<0.005). Daily mean energy intake was significantly higher in June(=1950.3㎉) & October (=1946.9㎉) than in February(=1423.3㎉)(p<0.005). According to RDA for koreans, the level of physical activity fell into exceptional activity category in June, heavy in October, moderate in February. Mean energy balance was negative in all seasons ; 0.682 in June, 0.812 in October and 0.698 in February. In three seasons mean body weight, mean obesity rate and mean body mass index(BMI) were not changed significantly. But there were significant seasonal variations in body fat(%) & lean body mass(LBM)(%). The mean percentage of body fat(%) was within normal range(24.44-24.79%) & the mean percentage of lean body mass(LBM)(%) representing long term physical activity was significantly higher in June(75.56%) & October(75.21%) & October (75.21%) than in February(72.75%)(p<0.05).

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Activity-oriented Modeling of Mass Production System (대량생산 체제의 Simulation을 위한 Activity 중심 Modeling)

  • Choe, Byeong-Gyu;Park, Seong-Ju;Sin, Ha-Yong
    • Journal of Korean Institute of Industrial Engineers
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    • v.12 no.1
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    • pp.119-131
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    • 1986
  • Described in this paper is a modelling methodology for mass production system simulation. The mass production system under consideration consists of various types of flow lines, special purpose production facilities, conveyor lines, palletized carts, and storage facilities. This type of production system is typical in home appliance industry, automobile industry, footwear industry, etc. where a variety of product mix are mass-produced. The modelling methodology is based on the "discrete-event formalism", and an "activity-oriented world view" is adopted to formalize the system description. A distinctive feature of the modelling methodology is that only the static structure (ie, system components) is included in the fixed model. The dynamic structure of the system is specified through a "data-driven" mechanism, which is an extension of the "experimental frame" concept. Each type of system components (ie, flow line, conveyors, carts, etc.) is formally modeled by using Activity Cycle Diagrams. The issue of "model structuring" is also addressed. The modeling methodology has been successfully applied in a real simulation study of a mass production system.

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Beneficial effects of body fat and obesity through the inhibition of the digestion of carbohydrate and lipid in gastrointestinal tract (장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구)

  • 정은희;윤승원;이홍석;윤유식;유경미;황인경
    • Korean journal of food and cookery science
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    • v.19 no.1
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    • pp.107-113
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    • 2003
  • In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3 kg of body weight and 3.5 kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.

Influence of Lifetime Sports Activity Based on a Ground Reaction Force on Bone Mineral Density in Korean Adults (지면반력에 근거한 과거 신체활동정도가 골밀도에 미치는 영향 -아이오와에 거주하는 한인을 대상으로-)

  • Lee Eun Nam;Clark Mary Kathleen
    • Journal of Korean Academy of Nursing
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    • v.35 no.3
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    • pp.621-630
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    • 2005
  • Purpose: The purpose of this study was to -retrospectively describe the childhood sports activity level of Korean adult men and women and to determine whether a higher level of childhood sports activity was positively associated with adult bone mineral density. Methods: A cross-sectional study of 100 Korean men (n=40) and women (n=60) was completed. Participants completed a detailed lifetime sports activity questionnaire and had their bone mineral density of the femur and lumbar spine measured using dual energy x-ray densitometry (DEXA). All sports activities were classified into four categories of peak strain score on the basis of ground reaction forces (GRF). Results: During the age of high school, women and men who participated in a high intensity sports activity demonstrated higher bone density in the femur site after adjustment for the effects of body weight, fat body mass, lean body mass, the level of calcium intake, and breast feeding period than those who did not participate in sports activity at all. Conclusion: These results highlight the need to participate in high intensity sports activity during high school age as a means of increasing peak bone mass in the femur site.