• Fisheries and Aquatic Sciences
• /
• 제12권1호
• /
• pp.6-15
• /
• 2009

Biochemical and functional properties of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from rockfish skin were characterized. Yield of PSC (90.0%) was higher than that of ASC (63.2%). Both ASC and the PSC consisted of ${\alpha}1$ and ${\alpha}2$ chains, and $\alpha$-cross-linked components. According to the results of hydroxylation of proline and lysine, and FT-IR, no difference between the helical structure of ASC and PSC was identified. Thermal denaturation temperature (TDT) of ASC from rockfish skin was $22.8^{\circ}C$, the same as exhibited in PSC. Both ASC and PSC were higher in water absorption capacity (WAC) and oil absorption capacity (OAC) than other vegetable proteins. According to the results of emulsifying activity (EA) and cooking stability (CS), both ASC and PSC from rockfish skin were inferior compared to the commercial emulsifier (Tween-80). The results of FT-IR suggested that the structure of PSC was slightly different when compared to that of ASC. No differences in solubility were established between ASC and PSC from rockfish skin at various pH and NaCl concentrations.

• 한국콘텐츠학회논문지
• /
• 제12권5호
• /
• pp.70-80
• /
• 2012

3D 입체공간에서 재미와 학습요소가 가미된 해양생물 문화콘텐츠를 개발하고, 도서해양 생물자원의 증강현실 콘텐츠 개발과 체험학습의 활용 방안을 제안한다. 증강현실 저작도구는 위지윅(WYSIWYG) 기반의 저작도구로 Node 구조와 Drag & Drop을 통해 저작도구를 쉽게 사용할 수 있도록 하였다. 해양생물 문화콘텐츠는 marker를 통한 애니메이션 효과, 모델링 데이터의 변화 등의 이벤트 요소를 추가하고, 해양생물 내레이션(narration)으로 실감 체험학습을 지원한다. 해양생물 AR Book은 50여종의 해양생물 증강현실 콘텐츠를 바탕으로 초등학교 수업 교재로 활용이 가능하며, 증강현실을 활용한 3D 입체영상 교육으로 실재적 관찰, 다양한 사고, 최대의 몰입감을 부여하여 학습효과를 높여준다.

• 한국식품영양과학회지
• /
• 제35권10호
• /
• pp.1484-1490
• /
• 2006

진주조개 가공 부산물인 패주의 효율적 이용과 더불어 신세대 기호에 맞는 패주 스파게티 소스를 제조하고 효율적으로 이용하고자 제조 조건의 구명을 시도하였고, 아울러 이의 품질 특성에 대하여 살펴보았다. 적정산도, 색도, 점도 및 관능검사의 결과로 미루어 보아 진주조개 패주 스파게티 소스의 제조를 위한 패주의 최적 첨가량은 11%로 판단되었고, 저장성 부여를 위한 적정 살균조건은 $F_0$ 값 4분으로 판단되었다. 최적조건에서 제조 및 살균처리한 진주조개 패주 스파게티 제품의 일반성분은 시판 스파게티 소스에 비하여 수분은 낮았고, 단백질과 회분의 경우 높아 차이가 있었다. 총 아미노산 함량은 진주조개 스파게티 소스가 시판 소스보다 높았으며, 주요 아미노산으로는 aspartic acid, glutamic acid 및 phenylalanine 등이었다. 또한, 진주조개 패주 스파게티 소스의 칼슘 및 인 함량은 각각 48.5 mg/100 g 및 27.1 mg/100 g이었고 칼슘/인 비율이 1.78로, 칼슘이 흡수되기 좋은 비율로 구성되었다. 유리아미노산 총함량 및 taste value는 진주조개 패주 스파게티 소스가 시판 소스에 비하여 낮았고, 두 제품 모두 맛에 관여하는 주요 유리아미노산은 glutamic acid 및 aspartic acid로 판단되었다.

• 한국식품영양과학회지
• /
• 제35권7호
• /
• pp.919-925
• /
• 2006

양식 굴을 효율적으로 이용할 목적으로 6가지의 상업적 효소(Alcalase, Flavourzyme, Neutrase, Protamex, pepsin, trypsin)를 이용하여 굴 효소 가수분해물을 제조하고, 그 특성에 대하여 살펴보았다. 상업적 효소 굴 가수분해물의 항산화능 및 ACE 저해능은 모두 Protamex로 1시간동안 가수분해시킨 것이 가장 우수하였고, 이 때 이들의 $IC_{50}$값은 각각 1.16 mg/mL 및 14 mg/mL이었다. 하지만 항균성은 모든 효소 가수분해물에서 인정되지 않았다. 또한, ACE 저해능 및 항산화능은 가수분해 시간에 따른 의존성이 인정되지 않았다. Protamex로 1시간동안 처리한 굴 가수분해물의 경우 가수분해 처리하지 않은 대조구에 비하여 $29{\sim}66\;kDa$ 획분과 6.5 kDa 부근의 획분이 모두 감소하여 저분자화 하는 경향을 나타내었다.

• Food Science and Biotechnology
• /
• 제16권4호
• /
• pp.549-556
• /
• 2007

The study was carried out to examine on the refiner discharge from Alaska pollock as a collagen resource by characterizing biochemical and functional properties of collagen. The refiner discharge from Alaska pollock surimi manufacturing was a good resource for collagen extraction according to the results of total protein, heavy metal, volatile basic nitrogen, collagen content, amino acid composition, and thermal denaturation temperature (TDT). TDT of acid soluble collagen from refiner discharge showed $20.7^{\circ}C$, which was similar to that of collagen from Alaska pollock muscle and was higher than that of collagen from Alaska pollock skin. TDT of acid-soluble collagen from refiner discharge was, however, lower than those of skin collagens from warm fish and land animal. Acid-soluble collagen from refiner discharge of Alaska pollock could be used as a functional ingredient for food and industrial applications according to the results of water and oil absorption capacities, and emulsion properties. In addition, if the thermal stability of the acid-soluble collagens is improved, collagen from refiner discharge from Alaska pollock could be more effectively used.

• 한국수산과학회지
• /
• 제40권6호
• /
• pp.343-349
• /
• 2007

This study examined the effective utilization of pearl processing by-products. Three extracts of hot-water extract (WE), hydro-cooked extract (HE), and two-step enzymatic hydrolysate (EH) were prepared from pearl oyster muscle, and their characteristics were examined. The moisture, crude protein, volatile basic nitrogen (VBN), and amino-N contents were 97.5-98.0%, 0.5-1.3%, 2.1-4.9 g/100 mL, and 35.0-74.5 g/100 mL, respectively. EH had the lowest VBN and highest amino-N contents. In addition, EH had the highest yields. In terms of its functional properties, EH inhibited angiotensin-I converting enzyme ($IC_{50}$, 1.39 mg/mL) more strongly than the other extracts ($IC_{50}$, 4.17-7.95 mg/mL). The free amino acid contents of WE, HE, and EH were 661, 470 and 1,150 mg/100 mL, respectively. Major amino acids were taurine and glutamic acid. Major inorganic ions were Na, Mg, and Ca. Contents of taste compounds, such as free amino acids, inorganic ions, and quaternary ammonium bases, differed significantly according to the extract methods. Based on the results of chemical experiments and sensory evaluation, the quality of EH was superior to the other extracts, and EH is suitable for use in natural flavoring materials.

• 한국수산과학회지
• /
• 제39권3호
• /
• pp.278-282
• /
• 2006

The powdered boil-dried oyster (C), the powdered smoke-dried oyster (OS-1) and the powdered smoke-dried oyster scrap (OS-2) were examined for their taste-active components, quality characteristics for potential utilization as a natural flavoring substance. The free amino acid level of OS-l and OS-2 were 395.6 mg/100 g and 551.5 mg/100 g, respectively, and that of betaine of OS-1 and OS-2 were 164.6 mg/100 g and 214.9 mg/100 g, respectively. The contents of inorganic ions were rich in Na, K, P, Ca and Mg in that order. Major free amino acids were taurine, glutamic acid, proline, alanine and glycine. The extract condition of the OS-1 and OS-2 the instant soup was the most appropriate at $98^{\circ}C$ for 1-5 min. The hot water extract of OS-1 and OS-2 with additives such as salt, sugar, pepper and onion powder had a good organoleptic qualities compared with the conventional flavoring substances in a local market. We conclude that powdered smoke-dried oyster and its scrap can be commercialized.

• Fisheries and Aquatic Sciences
• /
• 제22권2호
• /
• pp.6.1-6.10
• /
• 2019

Background: This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods: Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results: Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and $PGE_2$ production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-${\alpha}$, interleukin (IL)-6, and IL-$1{\beta}$). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-${\kappa}B$) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions: Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.

• 한국해양바이오학회지
• /
• 제9권2호
• /
• pp.43-48
• /
• 2017

Temperature is a major factor that affects the physiochemical properties of compounds. This study focus on the determination of thermostability and the effect of temperature on antioxidant activities of Ecklonia cava (E. cava) ethanoic extract. The ethanoic extracts of E. cava were evaluated at $30^{\circ}C$, $60^{\circ}C$ and $90^{\circ}C$ for 0 to 7 days. The antioxidant activities were determined by DPPH, hydroxyl radical and hydrogen peroxide scavenging activities. The intracellular reactive oxygen species (ROS) scavenging activity was investigated using DCFH-DA assay. Results revealed that the ethanoic extract of E. cava incubated at different temperatures for 0 to 7 days, showed stable scavenging activities on DPPH, hydroxyl radical and hydrogen peroxide. The ROS scavenging activities of ethanoic extract and ascorbic acid were also investigated. The extract showed a stable ROS scavenging activity from 0 to 7 day at $90^{\circ}C$. However, the scavenging activity of ascorbic acid at $90^{\circ}C$ decreased starting from day 3. These results indicated that the antioxidant effects of this food grade ethanoic extract of E. cava could remain stable during the employed temperatures of food processing.

• 한국수산과학회지
• /
• 제49권4호
• /
• pp.445-453
• /
• 2016

Seahorse Hippocampus abdominalis a marine teleost fish, has long been used as one of the essential materials in traditional Chinese medicine. However, the uses of seahorse have been limited due to its high cost, despite its beneficial biological activities. Seahorse has not been widely explored for its biofunctional properties and active components. In the present study, the enzymatic hydrolysates of seahorse were prepared by using two digestive enzymes (trypsin and pepsin) and five food grade enzymes (neutrase, protamex, alcalase, kojizyme, and flavourzyme). The enzymatic hydrolysates indicated higher hydrolysis yields than its water extract. Among them, the distilled water-pepsin hydrolysate (DP) which was obtained by distilled water extraction followed by pepsin hydrolysis, showed the highest yield and protein content as well as the highest alkyl radical scavenging activity. Also, it provided protective effects against oxidative stress induced by AAPH in vero cell and zebrafish. Further fractionation based on the molecular weight was carried out to identify it’s active components, and < 5 kDa (less than 5 kDa) molecular weight fraction was confirmed to have the highest antioxidant activity. In conclusion, this study suggests that DP of seahorse has antioxidant properties, and might be a novel and useful material from the marine origin for healthy functional foods and cosmetics.