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http://dx.doi.org/10.1186/s41240-019-0121-8

Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages  

Sanjeewa, K.K. Asanka (Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University)
Jayawardena, Thilina U. (Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University)
Kim, Hyun-Soo (Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University)
Kim, Seo-Young (Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University)
Ahn, Ginnae (Department of Food Technology and Nutrition, Chonnam National University)
Kim, Hak-Ju (Seojin Biotech Co. Ltd)
Fu, Xiaoting (College of Food Science and Engineering, Ocean University of China)
Jee, Youngheun (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Jeon, You-Jin (Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Fisheries and Aquatic Sciences / v.22, no.2, 2019 , pp. 6.1-6.10 More about this Journal
Abstract
Background: This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods: Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results: Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and $PGE_2$ production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-${\alpha}$, interleukin (IL)-6, and IL-$1{\beta}$). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-${\kappa}B$) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions: Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.
Keywords
Sargassum horneri; Ethanol extract; RAW 264.7 macrophages; Anti-inflammation; NF-${\kappa}B$; MAPKs;
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