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http://dx.doi.org/10.5657/KFAS.2016.0445

Antioxidant Activity of Pepsin Hydrolysate Derived from Edible Hippocampus abdominalis in vitro and in Zebrafish Models  

Kim, Hyun-Soo (Department of Marine Life Science, Jeju National University)
Shin, Byeung-Ok (Department of Marine Life Science, Jeju National University)
Kim, Seo-Young (Department of Marine Life Science, Jeju National University)
Wang, Lei (Department of Marine Life Science, Jeju National University)
Lee, WonWoo (Department of Marine Life Science, Jeju National University)
Kim, Yoon Taek (Center of Ornamental Reefs and Aquariums)
Rho, Sum (Center of Ornamental Reefs and Aquariums)
Cho, Moonjae (Department of Medicine, Medical School, Jeju National University)
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.4, 2016 , pp. 445-453 More about this Journal
Abstract
Seahorse Hippocampus abdominalis a marine teleost fish, has long been used as one of the essential materials in traditional Chinese medicine. However, the uses of seahorse have been limited due to its high cost, despite its beneficial biological activities. Seahorse has not been widely explored for its biofunctional properties and active components. In the present study, the enzymatic hydrolysates of seahorse were prepared by using two digestive enzymes (trypsin and pepsin) and five food grade enzymes (neutrase, protamex, alcalase, kojizyme, and flavourzyme). The enzymatic hydrolysates indicated higher hydrolysis yields than its water extract. Among them, the distilled water-pepsin hydrolysate (DP) which was obtained by distilled water extraction followed by pepsin hydrolysis, showed the highest yield and protein content as well as the highest alkyl radical scavenging activity. Also, it provided protective effects against oxidative stress induced by AAPH in vero cell and zebrafish. Further fractionation based on the molecular weight was carried out to identify it’s active components, and < 5 kDa (less than 5 kDa) molecular weight fraction was confirmed to have the highest antioxidant activity. In conclusion, this study suggests that DP of seahorse has antioxidant properties, and might be a novel and useful material from the marine origin for healthy functional foods and cosmetics.
Keywords
Seahorse; Hippocampus abdominalis; Enzymatic hydrolysis; Antioxidant activity; Zebrafish;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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