• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.47 no.4
• /
• pp.283-285
• /
• 2002

Momilactones A and B, the major phytotoxins and phytoalexins in rice plants, were quantified by a HPLC-APCI-MS-MS (APCI-MS-MS) system under multiple reaction monitoring conditions. Since MA and MB were found to be easily extracted with water, these phytotoxic compounds may affect germination and growth of other plant species when the rice straws were left in the fields.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.194-194
• /
• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2022.10a
• /
• pp.200-200
• /
• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.64 no.2
• /
• pp.1-13
• /
• 2022

Agrivoltaic facility is the composite system that the solar panel is installed above the farmland, and it enables crop and electricity production simultaneously. Solar panels of the agrivoltaic facilities can block and reduce the amount of solar irradiance arriving at the farmland, but it can help the crop growth by preventing excessive solar irradiance. Therefore, to clarify how the agrivoltaic facilities affect the crop growth, precise solar irradiance distribution under the solar panel should be modeled. In this study, PAR (photosynthetically active radiation), radiation from 400 to 700 nm, which crops usually use to grow, was extracted from the total irradiance and its distribution model under various conditions was developed. Monthly irradiance distributions varied because the elevation of the sun was changed over time, which made the position changed that the local maximum and minimum irradiance appear. The higher panel height did not cause any significant difference in the amount of irradiance reaching below the solar panel, but its distribution became more uniform. Furthermore, the panel angles with the most irradiance arriving below the solar panel were different by month, but its difference was up to 2%p between the irradiance with 30° angle which is usually recommended in Korea. Finally, the interval between panels was adjusted; when the ratio of the length of the panel to the empty space was 1:2, the irradiance of 0.719 times was reached compared to when there was no panel, 0.579 times for 1:1 and 0.442 times for 2:1.

• Journal of Korea Multimedia Society
• /
• v.23 no.10
• /
• pp.1250-1257
• /
• 2020

The early detection of diseases is important in agriculture because diseases are major threats of reducing crop yield for farmers. The shape and color of plant leaf are changed differently according to the disease. So we can detect and estimate the disease by inspecting the visual feature in leaf. This study presents a vision-based leaf classification method for detecting the diseases of tomato crop. ResNet-50 model was used to extract the visual feature in leaf and classify the disease of tomato crop, since the model showed the higher accuracy than the other ResNet models with different depths. We propose a new ensemble approach using several DCNN classifiers that have the same structure but have been trained at different ranges in the DCNN layers. Experimental result achieved accuracy of 97.19% for PlantVillage dataset. It validates that the proposed method effectively classify the disease of tomato crop.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1998.10a
• /
• pp.415-445
• /
• 1998

For effective prevention of the spreading and outbreak of crop insects and disease pests, an intensive Pest surveillance system was established to predict their density changes, and distribution. After their initial establishment by either immigration or overwintering, it is necessary to anticipate how they spread out geographically and predict where/when outbreaks are possible. The two major tools, boundary layer atmospheric model (Blayer) and the geographic information system(GIS), have been being developed to facilitate the prediction of pest occurrence in recent days. We are also developing the PeMos (Pest Monitoring System) that is able to manage the pest surveillance data collected from 152 pest monitoring stations in Korea. These three system related to the pest surveillance should be integrated into an internet based comprehensive database management system to facilitate information resources systematically organized and closely linked. Considering various data types and large data size in each system, a new special information management system is suggested. The integrated system should express complex types of information, such as text, multimedia, and other scientific data under the Internet environment. This paper discussed the major three systems, GIS, Blayer, and PeMos, relevant to the crop pest surveillance, then how they can be integrated in a comprehensive system under the Internet environment.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.37 no.4
• /
• pp.377-382
• /
• 1992

This study was conducted to obtain basic informations on the lignan components from sesame seed. Two major lignans, sesamin and sesamolin, were isolated and identified by means of spectral methods, and quantitative analysis was by HPLC from sesame variety Danbaeggae. Separation was achieved by isocratic elution and reversed phase chromatography Develosil ODS column. The content of the major lignan components were about 0.42% and 0.30% for sesamin and sesamolin, respectively.

• Korean Journal of Environmental Agriculture
• /
• v.40 no.3
• /
• pp.203-210
• /
• 2021

BACKGROUND: Pesticides can persist in soil due to multiannual uses. To decrease the concerns for potential carry-over of such residues from treated soil during preceding crop cultivation to non-target crops grown in rotation, an uptake study of procymidone was conducted under the actual cultivation conditions in Korea. METHODS AND RESULTS: Procymidone was sprayed twice by foliar application with recommended and double dose according to the safe use guidelines for Korean cabbage up to 14 days before harvest. Arable land was kept fallow for 4 days after harvesting Korean cabbage, and spinach was cultivated as a succeeding crop. Initial residues in soil were 5.670-14.175 mg/kg that were degraded to 3.098-4.555 mg/kg until harvest of Korean cabbage, and then persisted at 1.026-1.300 mg/kg by spinach harvest. Procymidone residues in edible part of succeeding crops from soil uptake were in range of 0.020-0.048 mg/kg for recommended dose and 0.055-0.116 mg/kg for double dose. Root concentration factor (RCF) values of procymidone at different concentration ranged from 0.053 to 0.123, and translocation factor (TF) ranged from 0.176 to 0.768 for spinach. The value of TF was higher than RCF, indicating that the capability of translocation to shoot was relatively higher than that of root uptake and accumulation. CONCLUSION: Procymidone applied on Korean cabbage can be carried-over to spinach and detected at a level similar to MRL (0.05^T mg/kg). Therefore, this study suggests a follow-up study for establishment of plant back interval (PBI) of succeeding crops reflecting the actual agricultural conditions as this study.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.64 no.4
• /
• pp.323-335
• /
• 2019

Rice is sensitive to day-length and short-day plants. It has a characteristic that the photosensitivity response required for flower bud differentiation decreases under long-day conditions. To identify critical photoperiod required for flower bud differentiation of major cultivation rice varieties, the average temperature was fixed at 28 ℃, and the day length was set at 12 hours and 10 minutes intervals from 13 hours to 14 hours 30 minutes. The critical photoperiod for each cultivar was set to day-length, where the daily cumulative response [(X_{(Critical Photoperiod)}-Y_{(Set day-length)})/(X_{(Critical Photoperiod)}-12:00_{(Optimal Day-length)}) × (28.0_{(Set Temperature)}-10_{(Minimum Temperature)})/(29.2_{(Maximum Temperature)}-10_{(Minimum Temperature)})] was the same for each day-length conditions. The flower bud differentiation time of all varieties was 32 days before heading at the average temperature of 28 ℃ conditions. The critical photoperiod of the early maturing type, such as Woonkwang, Odae, Koshihikari, Jopyeong, were 19:20, 18:14, 18:58, 17:30, respectively. Medium maturing type, such as Daebo, Haiami, Samdeok, were 16:08, 16:15, 16:55, respectively. Mid-late maturing type, such as Saenuri, Sindongjin, Chucheong, Samkwang, Ilpum, Saeilmi, Hwangkeumnuri, Dongjinchal, Ilmi, Hopum, Yeonghojinmi, were 15:58, 15:56, 16:36, 16:44, 15:35, 16:26, 15:33, 16:20, 16:29, 16:13, 15:41.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.263-263
• /
• 2017

Rice (Oryza sativa L.) is the world's third largest food crop after wheat and corn. Geographic authentication of rice has recently emerged as an important issue for enhancing human health via food safety and quality assurance. Here, we aimed to discriminate rice from six Asian countries through geographic authentication using combinations of elemental/isotopic composition analysis and chemometric techniques. Principal components analysis could distinguish samples cultivated from most countries, except for those cultivated in the Philippines and Japan. Furthermore, orthogonal projection to latent structure-discriminant analysis provided clear discrimination between rice cultivated in Korea and other countries. The major common variables responsible for differentiation in these models were ${\delta}^{34}S$, Mn, and Mg. Our findings contribute to understanding the variations in elemental and isotopic compositions in rice depending on geographic origins, and offer valuable insight into the control of fraudulent labeling regarding the geographic origins of rice traded among Asian countries.