• 가정∙방문간호학회지
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• 제26권2호
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• pp.219-229
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• 2019

Purpose: This study aimed to investigate the difference between primary caregivers' self-efficacy and coping strategy according to the communication styles of home care nurses. Methods: Data were collected from 123 primary caregivers of patients who were registered at a home care nursing center in D city and who had been receiving home care for more than 3 months from January 1 to February 27, 2018. The questionnaire included items on communication style, self-efficacy, and stress coping strategy. The data were analyzed using descriptive statistics, t-test, and ANOVA. Results: Regarding primary caregivers' self-efficacy in terms of communication style, the caregivers showed higher efficacy in providing informative and friendly communication (F=14.07, p=.001). Regarding home care nurses' communication style and the stress coping strategy of the primary caregivers, the informative-friendly communication style was adopted the most for the problem-solving coping strategy (F=7.17, p=.001). Regarding the social support-seeking coping, home care nurses' friendly communication style was the most adopted (F=4.40, p=.014). Conclusion: This study suggests that home care nurses will plan to provide informative and friendly communication-oriented nursing care, and to improve self-efficacy and positively influence the coping method by using the communication styles appropriate to the state of the primary caregiver.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.350-356
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• 2019

Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.

• 융합보안논문지
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• 제21권3호
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• pp.57-66
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• 2021

침입탐지 분야에서 딥러닝과 머신러닝을 이용한 탐지성능이 검증되면서 이를 활용한 사례가 나날이 증가하고 있다. 하지만, 학습에 필요한 데이터 수집이 어렵고, 수집된 데이터의 불균형으로 인해 머신러닝 성능이 현실에 적용되는데 어려움이 있다. 본 논문에서는 이에 대한 해결책으로 불균형 데이터 처리를 위해 t-SNE 시각화를 이용한 혼합샘플링 기법을 제안한다. 이를 위해 먼저, 페이로드를 포함한 침입탐지 이벤트에 대해서 특성에 맞게 필드를 분리한다. 분리된 필드에 대해 TF-IDF 기반의 피처를 추출한다. 추출된 피처를 기반으로 혼합샘플링 기법을 적용 후 t-SNE를 이용한 데이터 시각화를 통해 불균형 데이터가 처리된 침입탐지에 최적화된 데이터셋을 얻게 된다. 공개 침입탐지 데이터셋 CSIC2012를 통해 9가지 샘플링 기법을 적용하였으며, 제안한 샘플링 기법이 F-score, G-mean 평가 지표를 통해 탐지성능이 향상됨을 검증하였다.

• 대한한의학방제학회지
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• 제29권1호
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• pp.45-55
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• 2021

Objectives : This study was conducted to evaluate the anti-atrophic effect of polycan in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : C2C12 myoblast were differentiated into myotube by 2% horese serum medium for 6 days, and then treated polycan extract at different concentrations for 24h. The effect of dexamethasone on the induction of muscle atrophy and expression of atrophy-related genes in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : The results showed that Treatment with polycan (100 and 200 ㎍/㎖) noncytotoxic levels on both myoblast and myotube. Polycan decreased the ROS level overproduced with dexamethasone and improved the depletion of GSH level. Dexamethasone showed a decrease in myotube diameter, which was associated with up-regulation muscle-specific ubiquitin ligases markers, such as atrogin-1, FoxO3, myostatin and muscle RING finger-1 (MuRF1), and down-regulation of myogenin, MEF2, Myogenic regulatory factor 5, 6 and MyoD. The results showed that polycan treatment significantly dose-dependently inhibited it. Furthermore, decreased expressions of PI3K/Akt signal pathway by dexamethasone were reversed by treatment with polycan. Conclusions : Thus, polycan suppresses dexamethasone induced muscle atrophy in C2C12 myotube in vitro model through activation of PI3K/Akt pathway and protective effect of improve skeletal muscle function.

• 한국융합학회논문지
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• 제12권1호
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• pp.283-292
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• 2021

본 연구는 간호학생을 대상으로 재난간호 지식, 중증도 분류 지식 및 재난간호 준비도를 파악하고 재난간호 준비도에 미치는 영향요인을 파악하기 위한 서술적 조사연구이다. 자료수집은 2017년 6월 12일부터 10월 16일까지 B시와 G도의 간호학과 5곳의 3,4학년 간호학생 403명을 대상으로 실시하였다. 자가 보고식 설문지를 사용하였으며, IBM SPSS 25.0을 이용하여 기술통계, t-test, ANOVA, Scheffé test, Pearson's correlation coefficients, multiple regression으로 분석하였다. 연구결과 대상자의 재난간호 지식은 12.04±3.56점, 중증도 분류 지식은 6.24±2.64점, 재난간호 준비도는 79.98±12.16점이었다. 대상자의 재난간호 준비도에 영향을 미치는 요인은 낮은 재난간호 자신감(β=-.22, p<.001)과 재난간호 지식(β=.15, p=002)으로 확인되었으며, 모형의 설명력은 10.2%이었다. 그러므로, 간호학생들의 재난간호 준비도를 향상시키기 위해서는 재난간호에 대한 자신감을 향상시키고, 체계적이고 실용적인 재난간호 교육과정이 개발되어야 할 것이다.

• 한국전통조경학회지
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• 제29권1호
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• pp.121-130
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• 2011

본 연구는 조선 후기 개인문집 속에 묘사된 경관 관련 문헌을 조사 발굴하여 장소를 찾고, 그 속에 묘사된 대상지와 주변경관요소들을 분석하여 실제의 경관을 찾을 수 있는 가능성을 확인해 보는 기초연구로서, 대상지는 조선 후기 학자 최동집의 대암선생문집에 묘사된 농연서당십경에 대한 각 경관요소 및 장소를 찾아보는 것이다. 연구내용은 1) 대암 최동집과 대암선생문집을 정리한 후손 백불암 최흥원의 배경 및 농연서당에 대하여 이론적 고찰을 하였고, 2) 문헌자료 및 현장조사를 통해서 농연서당의 위치 및 장소를 파악하였으며, 3) 그 장소에서 농연서당십경에 대한 경관요소 및 분포관계 그리고 의미를 분석하였다. 고찰을 통해 농연서당의 배치와 방향에 대한 의미를 되짚고, 정원조성관련 기록을 확인하였으며, 지속적인 연구를 위한 방향을 제시하였다.

• 수면정신생리
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• 제28권1호
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• pp.18-26
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• 2021

Sleep is essential to brain function and mental health. Insomnia and obstructive sleep apnea (OSA) are the two most common sleep disorders, and are major public health concerns. Proton magnetic resonance spectroscopy (¹H-MRS) is a non-invasive method of quantifying neurometabolite concentrations. Therefore, ¹H-MRS studies on individuals with sleep disorders may enhance our understanding of the pathophysiology of these disorders. In this article, we reviewed ¹H-MRS studies in insomnia and OSA that reported changes in neurometabolite concentrations. Previous studies have consistently reported insomnia-related reductions in γ-aminobutyric acid (GABA) levels in the frontal and occipital regions, which suggest that changes in GABA are important to the etiology of insomnia. These results may support the hyperarousal theory that insomnia is associated with increased cognitive and physiological arousal. In addition, the severity of insomnia was associated with low glutamate and glutamine levels. Previous studies of OSA have consistently reported reduced N-acetylaspartate (NAA) levels in the frontal, parieto-occipital, and temporal regions. In addition, OSA was associated with increased myo-inositol levels. These results may provide evidence that intermittent hypoxia induced by OSA may result in neuronal damage in the brain, which can be related to neurocognitive dysfunction in patients with OSA. The current review summarizes findings related to neurochemical changes in insomnia and OSA. Future well-designed studies using ¹H-MRS have the potential to enhance our understanding of the pathophysiology of sleep disorders including insomnia and OSA.

• 농업과학연구
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• 제49권3호
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• pp.561-570
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• 2022

This study investigated the changes in planting environment and growing conditions of 'Arisoo' apple trees under shading net treatments. Apple tree canopies were fully covered with Blue (blue color net, mesh size: 4 mm × 5 mm), Black-A (black color net, mesh size: 4 mm × 5 mm), Black-B (black color net, mesh size: 5 mm × 6 mm), and uncovered trees were used as the control. Inside the tree canopies, the illuminance was observed as 100% (85.0 Klux) in the control, but it decreased to 75% (64.3 Klux) in the Blue, 73% (63.0 Klux) in the Black-A, and 77% (65.9 Klux) in the Black-B nets, respectively. The solar irradiance was observed as 755 w·m^-3 in the control, but it was decreased to 715 w·m^-3 in the Blue, 624 w·m^-3 in the Black-A, and 647 w·m^-3 in the Black-B nets, respectively. The average daily temperature was observed to be the highest in the Black-A net (31.8℃) while it was observed to be around 30.0℃ in the control, Blue, and Black-B nets. In addition, the average fruit surface temperature was observed to be the highest (39.4℃) in the control, but it was decreased to 38.1℃ in the Blue, 37.3℃ in the Black-A, and 39.0℃ in the Black-B nets. However, the average soil temperature, the shoot growth rate, and leaf SPAD values were not different in all the treatments. Fruit sunburn disorders were the highest in the control while it was observed to be lower in the fruits from trees covered with the shading nets. However, at harvest, the fruit quality parameters were not different in all the treatments. Overall, this study suggests that the apple trees covered with the shading nets reduced the illuminance, solar irradiance, fruit surface temperature, and fruit sunburn disorders. However, the shading nets did not affect tree growth and apple fruit quality.

• Biomolecules & Therapeutics
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• 제30권4호
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• pp.348-359
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• 2022

Gastric adenocarcinoma is among the top causes of cancer-related death and is one of the most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit the gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose of the present study was to investigate the effect of BITC on autophagy mechanism in AGS cells. First, the AGS cells were treated with 5, 10, or 15 μM BITC for 24 h, followed by an analysis of the autophagy mechanism. The expression level of autophagy proteins involved in different steps of autophagy, such as LC3B, p62/SQSTM1, Atg5-Atg12, Beclin1, p-mTOR/mTOR ratio, and class III PI3K was measured in the BITC-treated cells. Lysosomal function was investigated using cathepsin activity and Bafilomycin A1, an autophagy degradation stage inhibitor. Methods including qPCR, western blotting, and immunocytochemistry were employed to detect the protein expression levels. Acridine orange staining and omnicathepsin assay were conducted to analyze the lysosomal function. siRNA transfection was performed to knock down the LC3B gene. BITC reduced the level of autophagy protein such as Beclin 1, class III PI3K, and Atg5-Atg12. BITC also induced lysosomal dysfunction which was shown as reducing cathepsin activity, protein level of cathepsin, and enlargement of acidic vesicle. Overall, the results showed that the BITC-induced AGS cell death mechanism also comprises the inhibition of the cytoprotective autophagy at both initiation and degradation steps.

• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.915-925
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• 2023

Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.