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http://dx.doi.org/10.5713/ajas.18.0375

Effects of exercise on myokine gene expression in horse skeletal muscles  

Lee, Hyo Gun (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
Choi, Jae-Young (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
Park, Jung-Woong (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
Park, Tae Sub (Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University)
Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National, University)
Shin, Donghyun (Department of Animal Biotechnology, Chonbuk National, University)
Cho, Byung-Wook (Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.3, 2019 , pp. 350-356 More about this Journal
Abstract
Objective: To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skeletal muscle tissue was confirmed using quantitative-reverse transcriptase polymerase chain reactions (qRT-PCR). We then extracted horse muscle cells from the skeletal muscle tissue of a neonatal Thoroughbred. Myokine expression after hydrogen peroxide treatments was confirmed using qRT-PCR in horse skeletal muscle cells. Results: IL-6, IL-8, CXCL2, and CCL4 expression in Thoroughbred and Jeju horse skeletal muscles significantly increased after exercise. We stably maintained horse skeletal muscle cells in culture and confirmed the expression of the myogenic marker, myoblast determination protein (MyoD). Moreover, myokine expression was validated using hydrogen peroxide ($H_2O_2$)-treated horse skeletal muscle cells. The patterns of myokine expression in muscle cells were found to be similar to those observed in skeletal muscle tissue. Conclusion: We confirmed that several myokines involved in inflammation were induced by exercise in horse skeletal muscle tissue. In addition, we successfully cultured horse skeletal muscle cells and established an in vitro system to validate associated gene expression and function. This study will provide a valuable system for studying the function of exercise-related genes in the future.
Keywords
Exercise; Horse Skeletal Tissue; Horse Skeletal Muscle Cells; Myokine;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Yahiaoui L, Gvozdic D, Danialou G, Mack M, Petrof BJ. CC family chemokines directly regulate myoblast responses to skeletal muscle injury. J Physiol 2008;586:3991-4004.   DOI
2 Warren GL, O'Farrell L, Summan M, et al. Role of CC chemokines in skeletal muscle functional restoration after injury. Am J Physiol Cell Physiol 2004;286:C1031-6.   DOI
3 Pourteymour S, Eckardt K, Holen T, et al. Global mRNA sequencing of human skeletal muscle: search for novel exerciseregulated myokines. Mol Metab 2017;6:352-65.   DOI
4 Beavers KM, Brinkley TE, Nicklas BJ. Effect of exercise training on chronic inflammation. Clin Chim Acta 2010;411:785-93.   DOI
5 Niess AM, Simon P. Response and adaptation of skeletal muscle to exercise-the role of reactive oxygen species. Front Biosci 2007;12:4826-38.   DOI
6 Peake J, Nosaka K, Suzuki K. Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev 2005;11:64-85.
7 Seale P, Sabourin LA, Girgis-Gabardo A, et al. Pax7 is required for the specification of myogenic satellite cells. Cell 2000;102:777-86.   DOI
8 Poole DC. Current concepts of oxygen transport during exercise. Equine Comp Exerc Physiol 2004;1:5-22.   DOI
9 Kim H, Lee T, Park W, et al. Peeling back the evolutionary layers of molecular mechanisms responsive to exercise-stress in the skeletal muscle of the racing horse. DNA Res 2013;20:287-98.   DOI
10 Park KD, Park J, Ko J, et al. Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNASeq. BMC Genomics 2012;13:473.   DOI
11 Capomaccio S, Cappelli K, Barrey E, et al. Microarray analysis after strenuous exercise in peripheral blood mononuclear cells of endurance horses. Anim Genet 2010;41:166-75.   DOI
12 Eivers SS, McGivney BA, Fonseca RG, et al. Alterations in oxidative gene expression in equine skeletal muscle following exercise and training. Physiol Genomics 2010;40:83-93.   DOI
13 Park JW, Song KD, Kim NY, et al. Molecular analysis of alternative transcripts of equine AXL receptor tyrosine kinase gene. Asian-Australas J Anim Sci 2017;30:1471-7.   DOI
14 Cho HW, Shin S, Park JW, et al. Molecular characterization and expression analysis of the peroxisome proliferator activated receptor delta ($PPAR{\delta}$) gene before and after exercise in horse. Asian-Australas J Anim Sci 2015;28:697-702.   DOI
15 Park JW, Choi JY, Hong SA, et al. Exercise induced upregulation of glutamate-cysteine ligase catalytic subunit and glutamatecysteine ligase modifier subunit gene expression in Thoroughbred horses. Asian-Australas J Anim Sci 2017;30:728-35.   DOI
16 Pedersen BK, Akerstrom TC, Nielsen AR, Fischer CP. Role of myokines in exercise and metabolism. J Appl Physiol 2007;103:1093-8.   DOI
17 Jonsdottir I, Schjerling P, Ostrowski K, et al. Muscle contractions induces interleukin-6 mRNA production in rat skeletal muscles. J Physiol (Lond) 2000;528:157-63.   DOI
18 Akerstrom TC, Steensberg A, Keller P, et al. Exercise induces interleukin-8 expression in human skeletal muscle. J Physiol 2005;563:507-16.   DOI
19 Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK. Chemokines are elevated in plasma after strenuous exercise in humans. Eur J Appl Physiol 2001;84:244-5.   DOI
20 Peake JM, Roberts LA, Figueiredo VC, et al. The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise. J Physiol 2017;595:695-711.   DOI
21 Croisier JL, Camus G, Venneman I, et al. Effects of training on exercise-induced muscle damage and interleukin 6 production. Muscle Nerve 1999;22:208-12.   DOI
22 Pedersen BK, Steensberg A, Fischer C, et al. The metabolic role of IL-6 produced during exercise: is IL-6 an exercise factor? Proc Nutr Soc 2004;63:263-7.   DOI
23 Wolsk E, Mygind H, Grondahl TS, Pedersen BK, van Hall G. IL-6 selectively stimulates fat metabolism in human skeletal muscle. Am J Physiol Endocrinol Metab 2010;299:E832-40.   DOI
24 Steensberg A. The role of IL-6 in exercise-induced immune changes and metabolism. Exerc Immunol Rev 2003;9:40-7.
25 Koch AE, Polverini PJ, Kunkel SL, et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 1992;258:1798-801.   DOI
26 Catoire M, Mensink M, Kalkhoven E, Schrauwen P, Kersten S. Identification of human exercise-induced myokines using secretome analysis. Physiol Genomics 2014;46:256-67.   DOI
27 Bystry RS, Aluvihare V, Welch KA, Kallikourdis M, Betz AG. B cells and professional APCs recruit regulatory T cells via CCL4. Nat Immunol 2001;2:1126-32.   DOI
28 Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity 2000;12:121-7.   DOI