• Journal of Periodontal and Implant Science
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• 제37권4호
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• pp.859-869
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• 2007

Naked DNA and standard vectors have been previously used for gene delivery. Among these, PEI can efficiently condense DNA and has high intrinsic endosomal activities. The aim of this study is to investigate whether the cationic polycation PEI could increase the transfection efficiency of BMP expressing DNA using a vector-loaded collagen sponge model. BMP-2/pcDNA3.1 plasmid was constructed by subcloning human BMP-2 cDNA into the pcDNA3.1 plasmid vector. PEI/DNA complexes were prepared by mixing PEI and BMP-2/pcDNA3.1 and the constructed complexes were loaded into the collagen sponges. In vitro studies, BMSCs were transfected with the PEI/BMP-2/pcDNA3.1 complexes from collgen sponge. The level of secreted BMP-2 and alkaline phosphatase activities of transfected BMSCs were significantly higher in PEI/BMP-2/pcDNA3.1 group than in BMP-2/pcDNA3.1 group (p<0.05). Transfected BMSCs were cultured and mineralization was observed only in cells treated with PEI/BMP-2/pcDNA3.1 complexes. In vivo studies, PEI/BMP-2/pcDNA3.1/collagen, BMP-2/pcDNA3.1/collagen and blank collagen were grafted in skeletal muscle of nude mice. Ectopic bone formation was shown in PEI/BMP-2/pcDNA3.1/collagen grafted mouse 4 weeks postimplantation, while not in BMP-2/pcDNA3.1 grafted tissue. This study suggests that PEI-condensed DNA encoding for BMP-2 is capable of inducing bone formation in ectopic site and might increase the transfection rate of BMP-2/pcDNA3.1. As a non-viral vector, PEI offers the potential in gene therapy for bone engineering.

• 생명과학회지
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• 제26권2호
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• pp.155-163
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• 2016

젖산탈수소효소(Lactate dehydrogenase, EC 1.1.1.27, LDH) LDH-C의 기능을 확인하기 위해 liver-specific Ldh-C가 발현된 붕어(Carassius auratus)와 eye-specific Ldh-C가 발현된 파랑볼우럭(Lepomis macrochirus)을 기아 상태로 유지시킨 후(S) 조직들의 LDH 대사를 연구하였다. 기아 후 붕어 간조직의 LDH 활성이 크게 증가되었으며 LDH 비활성(units/mg)과 LDH/CS는 조직들에서 증가되어 혐기적 대사가 이루어짐을 확인하였다. 기아 후 LDH B₄ 동위효소가 골격근조직에서 감소되었고 심장조직에서 증가되었다. 눈과 뇌조직에 나타났던 LDH C₄ 동위효소는 liver-specific C₄로 확인되었으며 기아 후에 없어지고, 눈조직은 C hybrid, 뇌조직은 A₄, 간조직은 C hybrid와 C₄ 동위효소가 각각 증가되었다. 그러나 파랑볼우럭 조직에서 LDH 활성의 변화는 작았으나 눈조직에서 가장 크게 증가되었으며, 뇌조직은 LDH A₄와 AC hybrid가 증가되었다. 피루브산 10 mM에 의해 기아 후 붕어 조직의 LDH 활성은 30.30-18.64%, 파랑볼우럭 조직의 LDH는 25-18.75% 남았으며, 붕어는 Km^PYR 값이 증가되었다. 실험 결과 LDH liver-specific C 동위효소가 기아 중에 간, 뇌 및 눈조직에서 발현되었고, 기아 후 뇌조직에서 젖산의 대사가 우세하고, 붕어 LDH liver-specific C가 파랑볼우럭 LDH eye-specific C보다 영향을 더 받는 것으로 사료된다.

• 산업진흥연구
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• 제6권3호
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• pp.25-35
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• 2021

중년여성은 갱년기와 노화, 과다한 역할로 인해 다양한 심리적·신체적 스트레스로 긴장을 경험하며, 이것이 수면장애로 이어지는 경우가 많다. 그러나 이와 연관된 연구는 미비한 실정이다. 이에 수면장애를 경험하는 중년여성이 건강을 도모할 수 있도록 긴장이완을 돕는 싱잉볼의 효과성에 대한 연구 필요성이 제기된다. 이에 본 연구는 2019년 2월부터 2020년 4월까지 수면장애의 어려움을 호소하는 중년여성 중 피츠버그 수면의 질 평가 측정점수가 5점 이상인 4명을 선정하여 싱잉볼 체험을 40분 실시하고 심층 인터뷰 내용을 토대로 한 Giorgi의 현상학적 연구방법으로 진행하였다. 본 연구의 결과를 토대로 도출한 결론은 다음과 같다. 연구 결과 참여자들의 싱잉볼 체험을 통해 518개의 구성의미와 15개의 의미단위, 7개의 하위요소, 2개의 주제군을 도출해 내었다. 첫째, 싱잉볼 경험 시 신체적 경험으로는 통증과 이완 경험, 심리적 경험으로는 마음이완과 긍정심리 경험, 심상적 경험으로는 이미지와 색깔 경험을 하였다. 둘째, 싱잉볼 경험 이후 연구 참여자들이 느낀 신체적 변화는 신체이완, 신체증상, 신체활력 증가였으며 심리적 변화는 불안감소, 유쾌함과 긍정심리 증가였다. 행동적 변화는 행동 느긋해짐, 활동량의 증가였고 수면변화로 인한 수면개선의 효과가 두드러지게 나타났다. 본 연구에서 싱잉볼은 수면장애를 경험하는 중년여성의 긴장이완에 심리적·신체적으로 긍정적인 효과가 있음을 확인하였고, 스트레스와 긴장으로 인한 수면장애로 고통 받는 중년여성들과 현대인들에게 유용한 도구인 싱잉볼을 활용하여 양질의 삶을 살아가도록 돕는데 그 의의를 둔다.

• Archives of Plastic Surgery
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• 제34권5호
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• pp.617-621
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• 2007

Purpose: With the necessity of comprehensive care for the breast cancer patients, Breast Care Center of our hospital started to work in March 2004. This study examined the 3-year execution of Breast Care Center. The role of Breast Care Center was evaluated in aspect of clinical activities in quality and quantity. Methods: A retrospective review was carried out from 2001 to 2007 with breast cancer patients. The number and type of breast cancer surgery and breast reconstruction procedure were compared before and after Breast Care Center's foundation. Time required for the diagnosis and treatment was also compared. Regression analysis and T-test were used to identify the statistical significance. Results: The number of breast cancer surgery did not statistically increase compared with progressive increase of breast cancer. QUART(quadrantectomy, axillary dissection, radiotherapy) procedure statistically significantly increased and MRM(Modified Radical Mastectomy) procedure decreased for breast cancer surgery after foundation of Breast Care Center. The number of breast reconstruction procedures statistically significantly increased. The number of immediate TRAM free flap (Transverse Rectus Abdominal muscle free flap) procedures has grown 3.8 times after foundation of Breast Care Center. The time required for diagnosis and treatment was also shortened. Conclusion: After foundation of Breast Care Center, the number of breast reconstruction surgery increased, while the counselling time in breast reconstruction was reduced. Breast Care Center provided a prompt and comprehensive care to the breast cancer patients through the multidisciplinary therapeutic approaches.

• 한국전문물리치료학회지
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• 제13권3호
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• pp.92-101
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• 2006

The purpose of this study was to investigate the characteristics and the current condition of musculoskeletal pain sufferers among fitness center users. The investigation used self-reporting questionnaire with the 797 subjects. On average, the subjects were 30.6 years old, 168.8 cm in height and 65.2 kg in weight. Among them, 423 were male (53.1%) and 374 were female (46.9%). Out of them, 276 suffered from pain, (35.2%). More men felt muscle aches than women (p<.05). In terms of pain, lower back area topped the list with 44.9%, followed by the lower extremities (30.1%), the neck (17.8%) and the upper part of the body (17.8%). Dieters suffered the most from pain (86.2%) while those exercising to build strength suffered the least with 26.8%. There was a significant relationship between the purpose for exercising and the amount of pain suffered (p<.05). By kind of exercise, pain stroke aerobic exercisers disproportionately with 42.4% while it almost steered clear of exercisers for strengthening with 26.9%. There was significant relationship between the kind of exercise and pain suffered (p<.05). The type of suffering differed by body shape. A significantly higher number of overweight people experienced pain than underweight people (p<.05). 32.8% of pain sufferers commented that the pain affects their workout, and on this issue there was no gender difference (p>.05). The pain caused more difficulties in doing activities of daily living for overweight or obese peoples than underweight peoples (p<.05). More than a third of health club and fitness center users are experiencing musculoskeletal pains. Measures such as professional training or information provision is required to prevent injury or disorder caused by improper exercise.

• 한국축산식품학회지
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• 제36권3호
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• pp.359-368
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• 2016

The brown soybean extract (BE, extracted by distilled water, 50%, 75%, and 95% ethanol) were analyzed for their total phenol, flavonoid, anthocyanin content, and DPPH radical-scavenging activity to determine antioxidant activities. Brown soybean extract with 75% ethanol showed significantly higher DPPH radical scavenging activity, total phenol and anthocyanin content compared to the other treatments (p<0.05). Then, brown soybean extract with 75% ethanol was applied to pork patties at different concentration (0.05%, 0.1%, and 0.2%) and lipid oxidation was evaluated during 15 d of refrigerated storage. Addition of BE significantly increased redness and pH values, respectively (p<0.05). Moreover, TBARS value of pork patties decreased significantly (p<0.05) as BE concentration increased. In sensory evaluation, pork patties with 0.1% BE had significantly higher score than other treatments in flavor and overall acceptability (p<0.05). Consequently, these results indicate that 0.1% BE could be an effective natural antioxidant to inhibit lipid oxidation in pork patties.

• 디지털융복합연구
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• 제16권9호
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• pp.427-435
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• 2018

본 연구는 체지방률이 30% 이상인 비만 지적장애 성인여성들을 대상으로 노르딕워킹의 신체조성과 체력에 대한 효과를 검증하고자 하였다. 이를 위해, 21명의 대상자들을 실험군(12명)과 통제군(9명)으로 무작위 할당하여 구분한 후 12주 동안 실험을 진행하였다. 실험군은 주 2회의 노르딕워킹 프로그램에 참여하였으며, 통제군은 비교적 비활동적인 일상생활을 하게 하였다. 이러한 과정을 통해 다음과 같은 연구 결과를 얻었다. 신체조성에서 근육량과 체지방률이 실험집단에서 프로그램 참여 후에 유의한 개선 효과를 나타냈다. 또한 체력요인의 변화에서 있어서는 근력과 평형성, 협응력이 유의한 향상을 보였다. 이러한 결과를 통해 볼 때, 노르딕워킹 프로그램은 비만 지적장애 성인여성의 신체조성 변화와 체력 증진에 대체로 긍정적인 영향을 미치는 것으로 판단된다. 다만, 일부 요인에 대해서는 대상자의 특성이나 프로그램의 목표를 고려하여 운동 빈도와 운동 강도, 운동 내용 등을 조절할 필요가 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권6호
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• pp.755-764
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• 2017

The current review seeks to highlight the concerns that have been raised on pre-slaughter stress, contributing factors and its consequent effects on cattle behavioural responses and the quality of beef; inter-linking the activities involved from birth to slaughter. Such information is crucial in light of the consumer concerns on overall animal welfare, quality of meat and food security. Slaughter animals are exposed to different conditions during production and transportation to abattoirs on a daily basis. However; the majority of studies that have been done previously singled out different environments in the meat production chain, while conclusions have been made that the welfare of slaughter animals and the quality of meat harvested from them is dependent on the whole chain. Behaviour is a critical component used to evaluate the animals' wellbeing and it has been reported to have an effect on product quality. Apart from the influence of on-farm, transportation and abattoir conditions, the genetic background of the animal also affects how it perceives and responds to certain encounters. Stress activates the animals' hypothalamic-pituitary-adrenal activity, triggering release of various stress hormones such as catecholamines and cortisol, thus glycogen depletion prior slaughter, elevated ultimate pH and poor muscle-meat conversion. Pre-slaughter stress sometimes results to cattle attaining bruises, resulting to the affected parts of the carcass being trimmed and condemned for human consumption, downgrading of the carcass and thus profit losses.

• 환경생물
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• 제33권1호
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• pp.7-18
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• 2015

본 연구는 시멘트 노출에 따른 어류 조직의 형태 생리적 변화를 분석하여 용해된 시멘트 분말이 어류에 미치는 영향을 조사하고자 하였다. 용해된 시멘트 분말에 노출된 아가미는 일차새변의 두께가 두꺼워지고, 염세포와 점액세포도 두드러지게 활성이 증가되고 있으며, 이차새변의 새엽에서는 상피세포의 증식과 박리 및 곤봉화 현상도 관찰되었다. 신장 조직에서는 보우만 주머니 공간이 넓게 관찰되었고, 표피 조직은 표피층의 두께가 감소하며 진피층의 배열이 불규칙해지는 것으로 관찰되었다. 항산화효소와 LDH의 활성은 조직 및 노출 기간에 따라 활성에 차이가 있는 것으로 나타났다. 표피 조직에서 용해된 시멘트 분말에 의해 발현이 증가되는 단백질은 해당과정과 에너지 대사과정에 관여하는 단백질로 확인되었고 발현이 저하된 단백질들은 근수축에 관여하는 근섬유 구성 단백질로 조사되었다. 이러한 결과로 보아 용해된 시멘트 분말은 붕어 조직의 형태적 변형과 생리적 기능의 약화를 초래하여 어류의 생존에 커다란 위협이 될 요인으로 작용할 수 있을 것으로 사료된다.

• 대한물리치료과학회지
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• 제13권1호
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• pp.29-38
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• 2006

The purpose of this study lies in finding out the effect that variation of pain and body deflection posture has an influence on the static spinal stabilization after having performed spinal stabilization exercise making degenerative disc disease patients an object over 8 weeks using $CENTAUR^{(R)}$, 3-D spinal stabilization training implement. Subjects : 61 of DDD patients were made as an object of this study (mean age: 45.46 years, SD: ${\pm}12.78$, range: 16-68), their average height was 161.87cm, average weight 60.70kg, 12 males and 49 females were involved. Methods: 8 various investigations were performed and varied values were compared with reinvestigation done after having exercised 8 weeks using 3-D $CENTAUR^{(R)}$. We used VAS(Visual Analog Scale) in order to see the variation of pain intensity, MOS(Modified Oswestry Scale) in order to see activities of daily life. Results VAS was lessened from 7.50 to 2.71, limitation of routine life(MOS) from 20.26 to 9.32, there were remarkable differences statistically(p<0.05). As a result of muscular investigation for static spinal stabilization by 8 variations of body deflection, muscular strength were all increased and there were remarkable differences statistically(p<0.05). Conclusions : It has been turned out that pain and limitation of daily life was lessened as a result of making 61 of degenerative disc disease patients exercised 8 weeks using $CENTAUR^{(R)}$, 3-D spinal stabilization training implement, deep muscular power was increased. Thus it has been turned out that 3-D lumbar stabilization exercise has an effect on the spinal muscles strengthening and alleviation of their pain for degenerative disc disease.