• Proceedings of the Korean Magnestics Society Conference
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• 2007.05a
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• pp.95.2-95.2
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• 2007

• Journal of Microbiology and Biotechnology
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• v.31 no.6
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• pp.882-889
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• 2021

In order to use an enzyme industrially, it is necessary to increase the activity of the enzyme and optimize the reaction characteristics through molecular evolution techniques. We used the error-prone PCR method to improve the reaction characteristics of LipCA lipase discovered in Antarctic Croceibacter atlanticus. Recombinant Escherichia coli colonies showing large halo zones were selected in tributyrin-containing medium. The lipase activity of one mutant strain (M3-1) was significantly increased, compared to the wild-type (WT) strain. M3-1 strain produced about three times more lipase enzyme than did WT strain. After confirming the nucleotide sequence of the M3-1 gene to be different from that of the WT gene by four bases (73, 381, 756, and 822), the secondary structures of WT and M3-1 mRNA were predicted and compared by RNAfold web program. Compared to the mean free energy (MFE) of WT mRNA, that of M3-1 mRNA was lowered by 4.4 kcal/mol, and the MFE value was significantly lowered by mutations of bases 73 and 756. Site-directed mutagenesis was performed to find out which of the four base mutations actually affected the enzyme expression level. Among them, one mutant enzyme production decreased as WT enzyme production when the base 73 was changed (T→ C). These results show that one base change at position 73 can significantly affect protein expression level, and demonstrate that changing the mRNA sequence can increase the stability of mRNA, and can increase the production of foreign protein in E. coli.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.8
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• pp.3561-3569
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• 2012

This study investigated the effect of mulberry fruit extract(MFE) on antioxidant and anti-inflammatory activities of middle aged women with rheumatoid factor (RF). Thirty two middle-aged subjects were divided into two groups which were normal middle-aged group (NMG) and abnormal middle-aged group whose serum RF level were > 10 u/mL (AMG). All groups had consumed MFE (100 mL/day) for 4 weeks. Anthropometric measurements, serum inflammatory factors, serum oxidative stress markers analyses were performed at baseline and then at 4 weeks following the study. There were no significant differences in anthropometric measurements, including BMI, WHR and body fat composition between two groups. But after 4 weeks MFE consumption, serum levels of C-reactive protein (CRP), ferric-reducing ability of plasma (FRAP), serum TNF-${\alpha}$, IL-2, IL-4 had significantly decreased (p<0.05) in AMG. These findings suggested that the MFE consumption as food may be protective against oxidation and inflammation like RA.

• Molecules and Cells
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• v.20 no.1
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• pp.35-42
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• 2005

A novel combined method for locating box H/ACA small nucleolar RNAs (snoRNAs) is described, together with a software tool. The method adopts both a probabilistic hidden Markov model (HMM) and a minimum free energy (MFE) rule, and filters possible candidate box H/ACA snoRNAs obtained from genomic DNA sequences. With our novel method 12 known box H/ACA snoRNAs, and one strong candidate were identified in 30 nucleolar protein genomic sequences.

• Proceedings of the Korean Information Science Society Conference
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• 2002.04b
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• pp.589-591
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• 2002

MFEG-4 컨텐츠는 사용자 상호작용의 지원과 바이너리 포맷의 지원으로 분산 환경에서 다중 사용자가 공유하기에 적합하다. 기존의 가상 환경을 생성하는 장면 기술 언어들은 텍스트 기반 기술 언어이의로 네트워크를 기반으로 하는 공유 환경에 적합하지 않다. MPEG-4의 기술 언어인 BIFS는 바이너리 포맷이므로, 생성된 컨텐츠의 용량이 작아서 네트워크 기반의 공유에 적합하며, 2차인 및 3차원의 풍부한 컨턴츠 생성을 가능하게 한다. 본 논문에서는 MPEG-4의 BIFS를 이용하여 가상 환경을 생성하고, 생성된 컨텐츠론 다중 사용자가 공유하여 가상환경에 참여할 수 있도록 하는 재생기폰 제안한다. 본 논문에서는 MIEG-4 컨텐츠 공유를 위하여 필요한 부분을 분석하여 기술하고, 분석된 기능을 토대로 MFEG-4 재생기를 설계하였다. MFEG-4 컨텐츠 공유를 위하여 본 재생기는 컨텐츠 공유 세션의 설정을 지원하고, 컨텐츠에 입력되는 사용자 이벤트를 공유 세설에 참여하는 모든 사용자에게 전송한다. 또한 전송된 사용자 이벤트를 입력받은 원격지 재생기는 공유 메시지를 해석하여 MFE7-4 씬을 갱신한다. 이러한 MPEG-4기반 공유 컨텐츠는 다중 사용자웅 게임 컨텐츠와 교육용 컨텐츠, 방송용 컨텐츠 등에 활용 될 수 있다.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.2
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• pp.143-151
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• 1997

The purpose of this study was to investigate the effect of iron and selenium intakes on utilization of manganese in rats fed adequate, 2-fold, 4-fold iron and adequate, high selenium for 6 weeks. There was no difference feed intake across iron and selenium containing diet groups. Body weight gain in 2-fold iron and high selenium group(MFeHSe) was significantly higher than those in other groups. Serum iron level was increased with iron increment, and liver iron content was decreased with selenium supplementation. Selenium and manganese contents in tissues were decreased with iron increment. In the case of manganese balance, manganese excretion through feces was significantly increased as iron intake was increased. However, retention and apparent absorption of manganese were not significantly affected by dietary iron. From these results, it could be suggested that the supplementations of iron and selenium affected the manganese utilization. Therefore, it must be considered interaction with various minerals in micro-nutrient supplementations.

• Food Science and Preservation
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• v.9 no.1
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• pp.102-108
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• 2002

The purpose of this study was to investigate antibacterial activity of extracts of Cordyceps spp.(Paecilomyces japonica and Cordyceps militaris), mugwort and pine needle. Fruiting body and mycelium of Cordyceps spp., mugwort and pine needle were extracted with water and 70% ethanol. Antibacterial activities of each extracts against 3 kinds of Gram positive (Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus) and 3 kinds of Gram negative pathogenic bacteria(Escherichia coli O157 : H7, Shigella sonnei and Salmonella typhimurium) were tested. The yields of water and ethanol extracts of fruiting body (39∼58%) were 2.4 ∼4.4 times higher than mycellium(9∼24%) in Cordyceps sup., while those of mugwort and pine needle were less than 9%. Ethanol extract of P. japonica mycelium(JFE) had antibacterial to S. monocytogenes at 1% level and ethanol extract of C. militaris fruiting body (MFE) had antibacterial to S. aureus at 3% level. Ethanol extract of mugwort was antibacterial against L monocytogenes and S. aureus at 1% level. Water extracts of Cordyceps spp.(P. japonica and C. militaris) and mugwort had no antibacterial activity against tested bacterial strains. Water extract of pine needle had antibacterial activity against all bacterial strains except E. coli and ethanol extract had antibacterial activity against all tested bacterial strains at 1% level. Pine needle extracts had the most wide antibacterial spectrum against bacterial strains used for this experiment. Growth inhibiting activities of pine needle extracts were higher in ethanol extract than water extract for most of tested bacteria in tryptic soy broth.

• The KIPS Transactions:PartD
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• v.13D no.2 s.105
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• pp.175-182
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• 2006

Radars are used to detect the motion of the low flying enemy planes in the military. Radar-detected raw data are first processed and then inserted into the ground tactical C4I system. Next, these data we analyzed and broadcasted to the Shooter system in real time. But the accuracy of information and time spent on the displaying and graphical computation are dependent on the operator's capability. In this paper, we propose the Flying Object Tracking Management System that allows the displaying of the objects' trails in real time by using data received from the radars. We apply the coordinate system translation algorithm, existing communication protocol improvements with communication equipment, and signal and information computation process. Especially, radar signal duplication computation and synchronization algorithm is developed to display the objects' coordinates and thus we can improve the Tactical Air control system's reliability, efficiency, and easy-of-usage.

• The Journal of the Korea Contents Association
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• v.8 no.9
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• pp.10-18
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• 2008

The most critical element in the real-time operation of RFID application programs that are suitable for the mobile environment is the RFID middleware, made exclusively for mobile handsets, which processes collected data on a real-time basis and sends only the useful information to the application client. In this paper, we intend to design and implement a mobile RFID middleware system that supports the mobile handset environment for real-time based processing of the necessary data in the mobile environment. The proposed mobile RFID middleware system includes a mobile reader interface that collects the tag data, a mobile filtering engine that processes the tag data into more meaningful data that is sent to the application interface, and finally the mobile application interface that supports the m-SOAP application access protocol for interfacing with existing mobile applications. The filtering speed of the newly-developed middleware engine was found to be suitable for the characteristics of mobile handsets.

• Journal of Mushroom
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• v.16 no.4
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• pp.311-317
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• 2018

The aim of this study was to identify chemical composition and antioxidant activity of phenolic extract of fruiting bodies of an artificially cultivated Hankyong Sanghwang mushroom, Phellinus linteus KACC 93057P (PLHS). The total phenolic content of 60% ethanolic extract of fruiting bodies of two-year-old PLHS grown on Oak wood logs was $19.05{\pm}0.32mg$ gallic acid equivalent (GAE)/g, which was 4-10 times high compared to the other species of mushrooms. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) value of PLHS were 2-10 and 5 times higher, respectively, than those of the other species of mushrooms. High performance liquid chromatography (HPLC) analysis of ethyl acetate fraction of 70% methanolic extract of the fruiting bodies of PLHS revealed the presence of styrylpyrone-class compounds, davallialactone, hispidin, hypholomine B, and caffeic acid, a compound of the phenylpropanoid pathway.