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http://dx.doi.org/10.14480/JM.2018.16.4.311

Chemical identification and antioxidant activity of phenolic compounds extracted from the fruiting body of 'Hankyong Sanghwang', Phellinus linteus KACC 93057P  

Min, Gyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University)
Jeong, Ee-un (Graduate School of Future Convergence Technology, Hankyong National University)
Yun, Bong-Sik (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
Publication Information
Journal of Mushroom / v.16, no.4, 2018 , pp. 311-317 More about this Journal
Abstract
The aim of this study was to identify chemical composition and antioxidant activity of phenolic extract of fruiting bodies of an artificially cultivated Hankyong Sanghwang mushroom, Phellinus linteus KACC 93057P (PLHS). The total phenolic content of 60% ethanolic extract of fruiting bodies of two-year-old PLHS grown on Oak wood logs was $19.05{\pm}0.32mg$ gallic acid equivalent (GAE)/g, which was 4-10 times high compared to the other species of mushrooms. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) value of PLHS were 2-10 and 5 times higher, respectively, than those of the other species of mushrooms. High performance liquid chromatography (HPLC) analysis of ethyl acetate fraction of 70% methanolic extract of the fruiting bodies of PLHS revealed the presence of styrylpyrone-class compounds, davallialactone, hispidin, hypholomine B, and caffeic acid, a compound of the phenylpropanoid pathway.
Keywords
Phellinus linteus KACC 93057P; Fruiting body; Phenolic compound; Antioxidant activity; Chemical identification;
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1 Blois MS.1958. Antioxidant determination by the use of a stable free radical. Nature. 181: 1199-1200.   DOI
2 Chan KM, Decker EA, Means WJ. 1993. Extraction and activity of carnosine, a naturally occurring antioxodant in brrf muscle. J Food Sci. 58: 1-4.   DOI
3 Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS. 2010. Physiological activities of extract from edible mushrooms. J Kor Soc Food Sci Nutr. 39: 1087-1096.   DOI
4 Daniel S. 2010. Medicinal mushroom Phellinus linteus as an alternative cancer therapy (Review). Exp Ther Med. 1: 407-411.   DOI
5 Fridovich I. 1989. Superoxide dismutases. An adaptation to a paramagnetic gas. J Biol Chem. 264: 7761-7764.
6 Hong IP, Jung IY, Nam SH, Park JS, Cho JH, Lee HW, Guo SX. 2002. Cultural Characteristics of a medicinal mushroom, Phellinus linteus. Mycobiology. 30: 208-212.   DOI
7 Ikekawa T, Nakanishi M, Uehara N, Chihara G.1968. Antitumor action of some basidomycetes, especially Phellinus linteus. Gann. 59: 155-157
8 Ishikawa Y, Morimoto K, Hamasaki T. 1984. Flavoglaucin, a metabolite of Eurotium chevalieri, its antioxodation and synergism with tocopherol. J Am Oil Chem Soc.. 61: 1862-1868.
9 Kang HW, Lee MH, Seo GS. 2013.Antioxidant and Anti-inflammatory Effects of Phellinus linteus HN1009K. Kor J Mycol 41: 243-247   DOI
10 Kim HJ, Bae JT, Lee JW, Hwang Bo MH, Im HG, Lee IS, 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts. Kor J Food Preserv. 12: 80-85.
11 Jung JY, Lee IK, Seok SJ, Lee HJ, Lee HJ, Kim YH, Yun BS. 2008. Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus. J Applied Microbiol. 104: 1824-1832   DOI
12 Kim JO, Jung MJ, Choi HJ, Lee JT, Lim AK, Hong JH, Kim DI. 2008. Antioxidative and biological activity of hot water and ethanol extracts from Phellinus linteus. J Kor Soc Food Sci Nutr. 37: 684-690.   DOI
13 Lee IK, Jung JY, Kim YH, Yun BS. 2009. Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: II. Physicochemical properties and structure elucidation. J Antibiotics. 62: 635-637.   DOI
14 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radicals Biol Med. 26.
15 Lee IK, Seo GS, Jeon NB, Kang HW, Yun BS. 2009. Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: I. Fermentation, taxonomy, isolation and biological properties. J Antibiotics. 62: 631-634.   DOI
16 Lee YG, Lee WM, Kim JY, Lee JY, Lee IK, Yun BS, Rhee MH, Cho JY. 2008. SRC kinase-targeted anti-inflammatory activity of davallialactone from Inonotus xeranticus in lipopolysaccharide activated RAW264.7 cells. Br J Pharmacol. 154:852-63.
17 Min GJ, Kwak AM., Seok SJ, Kang HW. 2016. Morphological and cultural characteristics of a novel Phellinus linteus KACC93057P. J Mushrooms. 14; 75-80.   DOI
18 Videla LA Fermandaz V. 1988. Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp. 21: 85-92.
19 WU SH, Dai YC, Hattori T, Yu TW, Wang DM Parmasto E, Chan HY, Shih SY .2012. Species clarification for the medicinally valuable'sanghuang' mushroom. Bot Studies. 53: 135-149.