• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.432-441
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• 2016

Machaerium cuspidatum, a canopy liana, is a species of genus legume in the Fabaceae family and contributes to the total species richness in the tropical rain forests. In the present study, we investigated the antioxidative and anti-cancer effects of M. cuspidatum and its mode of action. The methanol extract of M. cuspidatum (MEMC) exhibited anti-oxidative activity with an $IC_{50}$ value of $1.66{\mu}g/ml$, and this was attributable to its 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. MEMC also exhibited a cytotoxic effect and induced morphological changes in a dose-dependent manner in several cancer cell lines including human lung adenocarcinoma A549 cells, human hepatocellular carcinoma HepG2 cells, and human colon carcinoma HT29 cells. Moreover, MEMC treatment induced the accumulation of subG1 population, which is indicative of apoptosis in A549 and HepG2 cells. MEMC-induced apoptosis was confirmed by the increase in Annexin V-positive apoptotic cells and apoptotic bodies using Annexin-V staining and DAPI staining, respectively. Further investigation showed that MEMC-induced apoptosis was associated with the increase in p53 and Bax expression, and the decrease in Bcl-2 expression. In addition, MEMC treatment led to proteolytic activation of caspase-3, 8, and 9 and degradation of poly-ADP ribose polymerase (PARP). Taken together, these results suggest that MEMC may exert a beneficial anti-cancer effect by inducing apoptosis via both the extrinsic and intrinsic pathways in A549 and HepG2 cells.

• Fisheries and Aquatic Sciences
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• v.26 no.1
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• pp.35-47
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• 2023

Myelophycus caespitosus, a brown alga belonging to genus Myelophycus, has been traditionally used as a food and medicinal resource in Northeastern Asia. However, few studies have been conducted on its pharmacological activity. In this study, we evaluated whether methanol extract of M. caespitosus (MEMC) could protect against oxidative damage caused by hydrogen peroxide (H₂O₂) in C2C12 murine myoblasts. Our results revealed that MEMC could suppress H₂O₂-induced growth inhibition and DNA damage while blocking the production of reactive oxygen species. In H₂O₂-treated cells, cell cycle progression was halted at the G2/M phase, accompanied by changes in expression of key cell cycle regulators. However, these effects were attenuated by MEMC. In addition, we found that MEMC protected cells from induction of apoptosis associated with mitochondrial impairment caused by H₂O₂ treatment. Furthermore, MEMC enhanced the phosphorylation of nuclear factor-erythroid-2 related factor 2 (Nrf2) and expression and activity of heme oxygenase-1 (HO-1) in H₂O₂-treaetd C2C12 myoblasts. However, such anti-apoptotic and cytoprotective effects of MEMC were greatly abolished by HO-1 inhibitor, suggesting that MEMC could increase Nrf2-mediated activity of HO-1 to protect C2C12 myoblasts from oxidative stress.

• The Safety technology
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• no.53
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• pp.43-45
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• 2002

• Proceedings of the Korean Society for Quality Management Conference
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• 2004.04a
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• pp.472-483
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• 2004

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.18 no.4
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• pp.49-59
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• 2004

In this paper the automatic diameter control system of the CZ-150 Crystal Grower is proposed in order to achieve a good performance for the industry applications in MEMC Korea. The effectiveness of the design parameters is verified by means of the computer simulations and the proposed controllers showed the better performance than the conventional PD controllers which always have steady-state errors.

• Proceedings of the Korean Information Science Society Conference
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• 2003.10b
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• pp.649-651
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• 2003

사람들의 욕구가 인터넷에 반영되면서 텍스트로 이루어져있는 인터넷 컨턴츠들이 이제는 멀티미디어로 이루어지고 있다. 사람들이 가장 많이 사용하고 있는 이메일 또한 이런 욕구가 반영되었다. 이젠 이메일도 멀티미디어 시대이다. 이메일의 장점을 이용해서 마케팅을 해오던 회사들도 텍스트가 아니라 멀티미디어로 사람들의 시선을 잡고자 한다. 기업의 마케팅 메일은 이메일을 사용하는 사용자 입장에서 본다면 스팸 메일이다. 많은 이메일 서비스 업체는 이런 스팸 메일을 차단하기 위해서 끈임없는 노력을 하고 있으며 많은 성과를 이뤘다. 현재는 과거의 방법으로 스팸 메일을 차단할 수 없기 때문에 새로운 방법이 모색되고 있다. 본 논문에서는 멀티미디어 스팸 메일 차단에 한 방법으로 MPEG-7를 이용한 MEMC(Multimedia E-Mail Classifictaion)을 제안한다.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.1
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• pp.13-20
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• 2004

In this paper, we have analyzed the current MPEG-4 video encoding tools and proposed efcient coding techniques that reduce the complexity of the encoder. Until recently, encoder optimization without shape coding has been a major concern in video for wire/wireless low bit rate coding services. Recently, we found out that the computational complexity of MPEG-4 shape coding plays a very important role in the object-based coding through experiments. We have made an experiment whether we could get optimized object-based coding method through successfully combining latest optimized texture coding techniques with our proposed optimized shape coding techniques. In texture coding, we applied the MVFAST method for motion estimation. We chose not to use IVOPF(Intelligent VOP Formation) but to use TRB(Tightest Rectangular Boundary) for positioning VOP and, finally, to eliminate the spiral search of shape motion estimation to reduce the complexity in shape coding. As a result of experiment, our proposed scheme achieved improved time complexity over the existing reference software by $57.3\%$ and over the optimized method on which only shape coding was applied by $48.7\%$, respectively.

• Proceedings of the IEEK Conference
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• 2003.07c
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• pp.2799-2802
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• 2003

The automatic diameter control system of the Crystal Grower has a good performance with only PD control. But it contained the integrator the plant which has a long time delay. In this paper. we show the secondary approximate model and applies time delay controller which is good performance for in the long time delay plant. It will be able to improve the response characteristic against a standard input and a load disturbance.

• Proceedings of the IEEK Conference
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• 2003.07c
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• pp.2803-2806
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• 2003

In this paper considers the design of the effective controllers for the plant with an integrator and a long dead time. The structure of the new DTC(Dead Time Compensator) that has an additive filter is proposed. Our DTC shows the improved performance in set-point response and disturbance rejection. Especially the bad effect of the long dead time in the disturbance rejection is fairly reduced by using the additive filter. illustrative examples are provided to show the availability of the proposed controller.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.327-330
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• 2005

In this paper, we proposed that technique for reducing trailing artifact in MPEG-4 video. low bit-rate video is occured trailng artifact which remain part of moving object. Because MEMC doesn't work well in the degraded video. The proposed trailing artifact reduction approach has at its core three main steps. First step is to detect trailing artifact. Second step is applied intra block coding to remove trailing artifact. Finally, bit-rate control algorithm is used. Experimental result using an MPEG-4 based encoder indicate that the proposed method has overhead(bits) of about 2 percent and proposed method effective reduce or eliminate trailing artifact