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http://dx.doi.org/10.4014/mbl.1608.08003

Anti-oxidative and Anti-cancer Activities of Methanol Extract of Machaerium cuspidatum  

Jin, Soojung (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Oh, You Na (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Park, Hyun-jin (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.4, 2016 , pp. 432-441 More about this Journal
Abstract
Machaerium cuspidatum, a canopy liana, is a species of genus legume in the Fabaceae family and contributes to the total species richness in the tropical rain forests. In the present study, we investigated the antioxidative and anti-cancer effects of M. cuspidatum and its mode of action. The methanol extract of M. cuspidatum (MEMC) exhibited anti-oxidative activity with an $IC_{50}$ value of $1.66{\mu}g/ml$, and this was attributable to its 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. MEMC also exhibited a cytotoxic effect and induced morphological changes in a dose-dependent manner in several cancer cell lines including human lung adenocarcinoma A549 cells, human hepatocellular carcinoma HepG2 cells, and human colon carcinoma HT29 cells. Moreover, MEMC treatment induced the accumulation of subG1 population, which is indicative of apoptosis in A549 and HepG2 cells. MEMC-induced apoptosis was confirmed by the increase in Annexin V-positive apoptotic cells and apoptotic bodies using Annexin-V staining and DAPI staining, respectively. Further investigation showed that MEMC-induced apoptosis was associated with the increase in p53 and Bax expression, and the decrease in Bcl-2 expression. In addition, MEMC treatment led to proteolytic activation of caspase-3, 8, and 9 and degradation of poly-ADP ribose polymerase (PARP). Taken together, these results suggest that MEMC may exert a beneficial anti-cancer effect by inducing apoptosis via both the extrinsic and intrinsic pathways in A549 and HepG2 cells.
Keywords
Anti-cancer; anti-oxidative; apoptosis; A549; HepG2; Machaerium cuspidatum;
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